FastQCFastQC Report
Thu 26 May 2016
SRR1545903_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545903_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2266950
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135110.595999029533073No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA55110.24310196519552701No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA46370.20454796091665012No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA44550.19651955270297097No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA25640.11310350912018349No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGTA302.5288118E-644.023
CTCCGAT207.858105E-444.022
CGTGATA207.858105E-444.013
CATTGCG302.5288118E-644.01
CACGCTA254.4442146E-544.019
CGTCTAC207.858105E-444.023
CCGCTTA351.4469151E-744.026
CGCTCGA351.4469151E-744.027
TATTACG700.044.01
ACTCGAT408.3182385E-944.020
TCGTACC351.4469151E-744.015
ACGTAGA254.4442146E-544.023
CCAACGG254.4442146E-544.02
CGGTATA207.858105E-444.023
TCGAATC254.4442146E-544.037
GTACGAT302.5288118E-644.025
GTTGCGA254.4442146E-544.038
CTTACGA207.858105E-444.028
CTATACG950.041.684211
TCGTTAG1700.041.4117621
Produced by FastQC (version 0.11.5)