##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545903_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2266950
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.717659851342113	31.0	31.0	33.0	30.0	34.0
2	32.13630340325106	33.0	31.0	34.0	30.0	34.0
3	32.21178984979819	34.0	31.0	34.0	30.0	34.0
4	35.892257438408436	37.0	35.0	37.0	35.0	37.0
5	35.86834160435828	37.0	35.0	37.0	35.0	37.0
6	35.91738326826794	37.0	35.0	37.0	35.0	37.0
7	36.11266371115375	37.0	35.0	37.0	35.0	37.0
8	36.05281148679944	37.0	35.0	37.0	35.0	37.0
9	37.77856106222016	39.0	38.0	39.0	35.0	39.0
10	37.562428814045305	39.0	37.0	39.0	35.0	39.0
11	37.30206224221972	39.0	37.0	39.0	34.0	39.0
12	36.6146059683716	39.0	35.0	39.0	33.0	39.0
13	36.39707315997265	39.0	35.0	39.0	33.0	39.0
14	37.441324246233926	40.0	35.0	41.0	33.0	41.0
15	37.616945234786826	40.0	35.0	41.0	33.0	41.0
16	37.68578927634046	40.0	35.0	41.0	33.0	41.0
17	37.60909636295463	39.0	35.0	41.0	33.0	41.0
18	37.551348728467765	39.0	35.0	41.0	33.0	41.0
19	37.47549571009506	39.0	35.0	41.0	33.0	41.0
20	37.32301241756545	39.0	35.0	41.0	33.0	41.0
21	37.20516023732328	39.0	35.0	41.0	33.0	41.0
22	37.19766602704074	39.0	35.0	41.0	33.0	41.0
23	37.199517413264516	39.0	35.0	41.0	33.0	41.0
24	37.145390943779084	39.0	35.0	41.0	33.0	41.0
25	37.05886940603013	38.0	35.0	41.0	33.0	41.0
26	36.95382474249542	38.0	35.0	41.0	33.0	41.0
27	36.86253600652859	38.0	35.0	41.0	32.0	41.0
28	36.85760338781182	38.0	35.0	41.0	32.0	41.0
29	36.81946756655418	38.0	35.0	41.0	32.0	41.0
30	36.71298617084629	38.0	35.0	41.0	32.0	41.0
31	36.56457310483248	38.0	35.0	41.0	31.0	41.0
32	36.38247866075564	38.0	35.0	41.0	31.0	41.0
33	36.23353977811597	38.0	35.0	41.0	31.0	41.0
34	36.0813427733298	38.0	35.0	41.0	30.0	41.0
35	35.884636185182735	38.0	35.0	41.0	30.0	41.0
36	35.76396788636715	38.0	35.0	41.0	30.0	41.0
37	35.70969408235735	38.0	35.0	41.0	29.0	41.0
38	35.64394097796599	38.0	35.0	40.0	29.0	41.0
39	35.59691700302168	38.0	35.0	40.0	29.0	41.0
40	35.448671563113436	38.0	35.0	40.0	28.0	41.0
41	35.36854010895697	38.0	35.0	40.0	28.0	41.0
42	35.31727078232868	38.0	35.0	40.0	27.0	41.0
43	35.24630494717572	37.0	35.0	40.0	27.0	41.0
44	35.128399391252564	37.0	34.0	40.0	27.0	41.0
45	35.01629899203776	37.0	34.0	40.0	27.0	41.0
46	34.974350117999954	37.0	34.0	40.0	27.0	41.0
47	34.91870751450186	37.0	34.0	40.0	26.0	41.0
48	34.839444628245	36.0	34.0	40.0	26.0	41.0
49	34.78624892476676	36.0	34.0	40.0	26.0	41.0
50	34.615435717594124	36.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	5.0
13	14.0
14	21.0
15	55.0
16	120.0
17	325.0
18	664.0
19	1357.0
20	2569.0
21	4185.0
22	6421.0
23	9458.0
24	13891.0
25	20079.0
26	28499.0
27	35384.0
28	37618.0
29	40411.0
30	45638.0
31	54786.0
32	67535.0
33	90105.0
34	175726.0
35	358907.0
36	131822.0
37	168576.0
38	292426.0
39	680351.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.942896843776886	22.23264738966453	29.37934228809634	13.44511347846225
2	31.353272017468402	25.947330113147622	29.859723416925828	12.83967445245815
3	29.67476124307991	25.35777145503871	32.10458986744304	12.86287743443834
4	27.509473080570814	26.660358631641635	31.404353867531263	14.425814420256291
5	23.535763911863956	32.33057632501819	30.085489313835772	14.048170449282074
6	21.053794746244954	42.01998279626811	27.25349037252696	9.67273208495997
7	86.40283199894131	4.58708837865855	7.492401685083482	1.517677937316659
8	87.29738194490395	4.033878118176404	6.940206003661308	1.7285339332583427
9	81.56752464765434	6.675665541807274	8.785681201614503	2.9711286089238844
10	47.861267341582305	27.323496327664927	14.2534242043274	10.561812126425373
11	41.01559363903042	21.80043671011712	24.232029819801937	12.95193983105053
12	36.680694324974084	22.789077835858755	26.887315556143715	13.642912283023445
13	21.062661284986437	37.687200864597806	26.399126579765763	14.851011270649991
14	15.785747369814068	39.79408456295904	29.519751207569644	14.900416859657248
15	15.658175081056044	23.885661351154635	45.90335031650456	14.552813251284766
16	18.0969143562937	21.37876883036679	41.95743179161428	18.566885021725227
17	17.686715631134344	21.213833564922034	29.940007499062617	31.15944330488101
18	21.68305432409184	23.871986589911558	34.823132402567325	19.621826683429276
19	28.972716645713405	23.84472529169148	27.924480028231763	19.25807803436335
20	30.066476984494585	23.989413088069874	26.85824566046891	19.085864266966627
21	22.163832462118705	28.779417278722512	29.371578552680916	19.685171706477867
22	21.605064072873244	25.32499614018836	27.71843225479168	25.351507532146716
23	19.846842674077507	29.540660358631644	27.833520809898765	22.77897615739209
24	18.87950770859525	24.808487174397317	38.91170956571605	17.400295551291382
25	17.877368270142703	26.133880323783053	36.308167361432766	19.680584044641478
26	17.073071748384393	34.01376298550917	29.05891175367785	19.854253512428592
27	18.738790004190655	32.20582721277487	30.456207679922365	18.599175103112113
28	15.763426630494717	28.091532676062553	38.54368203974503	17.601358653697698
29	17.06129380886213	25.31246829440438	37.21374534065595	20.41249255607755
30	18.218443282824943	30.85096715851695	32.13096892300227	18.799620635655838
31	28.61126182756567	26.120999580934733	26.50905401530691	18.75868457619268
32	28.387525088775668	26.746421403206956	27.86281126623878	17.0032422417786
33	26.16237676172831	27.54586559033062	26.86323033150268	19.428527316438387
34	19.025254196166657	27.796554842409403	29.639912657976573	23.538278303447363
35	20.757537660733586	25.768146628730232	32.4404596484263	21.033856062109884
36	28.015792143629103	27.12631509296632	27.173382738922342	17.684510024482233
37	20.031275502326913	34.015174573766515	28.38959835902865	17.563951564877918
38	20.106133792099516	34.07512296257085	25.995588786695777	19.823154458633844
39	20.96667328348662	32.52599307439511	27.330510156818633	19.176823485299632
40	23.63598667813582	27.93969871413132	26.61765808685679	21.806656520876068
41	18.409537043163724	26.17922759655043	28.183021239992062	27.228214120293785
42	20.9018284479146	26.600939588433796	26.490615143695273	26.006616819956328
43	21.86819294646993	26.171640309667172	27.479212157303866	24.480954586559033
44	19.522662608350426	29.94706544034937	29.964357396501907	20.565914554798297
45	18.104633979576082	36.012571957917025	25.152341251461213	20.730452811045676
46	20.645272282141203	33.89571891748825	26.664284611482387	18.794724188888154
47	21.54048391009947	27.643441628619954	28.326076887447893	22.48999757383268
48	21.284457089922583	26.097620150422372	32.10185491519442	20.51606784446062
49	20.725335803612786	25.933743576170627	33.27254681400119	20.0683738062154
50	19.84128454531419	32.46851496504114	28.586162023864663	19.10403846578001
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2441.0
1	4876.0
2	7311.0
3	16276.5
4	25242.0
5	18048.0
6	10854.0
7	10730.0
8	10606.0
9	10553.0
10	10500.0
11	10124.0
12	9748.0
13	9443.0
14	9138.0
15	8299.0
16	7460.0
17	7318.0
18	7176.0
19	7220.0
20	7264.0
21	9436.5
22	11609.0
23	12839.5
24	14070.0
25	17394.0
26	20718.0
27	27025.5
28	33333.0
29	38668.5
30	44004.0
31	50334.0
32	56664.0
33	57878.0
34	59092.0
35	65536.5
36	71981.0
37	75743.5
38	79506.0
39	95987.5
40	112469.0
41	147466.5
42	182464.0
43	205729.5
44	228995.0
45	232371.5
46	235748.0
47	220127.0
48	204506.0
49	193572.5
50	182639.0
51	168093.0
52	153547.0
53	138014.5
54	122482.0
55	109480.0
56	96478.0
57	88340.5
58	80203.0
59	69814.0
60	59425.0
61	50162.5
62	40900.0
63	33523.5
64	26147.0
65	20653.0
66	15159.0
67	12519.0
68	9879.0
69	8395.0
70	6911.0
71	5580.0
72	4249.0
73	3414.5
74	2580.0
75	2151.5
76	1723.0
77	1226.0
78	729.0
79	587.5
80	446.0
81	378.5
82	311.0
83	201.0
84	91.0
85	72.5
86	54.0
87	52.0
88	50.0
89	41.0
90	32.0
91	23.0
92	14.0
93	7.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2266950.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.068766406270365
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.34623736519704	11.956499160726356
2	6.814754099103393	2.053798752711249
3	2.24201585064369	1.0135323939751593
4	1.1861759314476488	0.7149683211089916
5	0.7995893359078586	0.6024412461870186
6	0.596259050888021	0.5390933013273637
7	0.45329831662294157	0.4781452511882682
8	0.3895227638348472	0.46957020305217045
9	0.33720214461179504	0.45730983139636844
>10	4.396746046853403	15.833675273109172
>50	1.552994383459385	17.18809100165373
>100	1.87010412398187	45.71789607558775
>500	0.011547508048500923	1.1767314722050601
>1k	0.0029608994996156214	0.9495411841763892
>5k	2.9608994996156214E-4	0.245884854148862
>10k+	2.9608994996156214E-4	0.6028216774460669
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13511	0.595999029533073	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	5511	0.24310196519552701	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	4637	0.20454796091665012	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	4455	0.19651955270297097	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	2564	0.11310350912018349	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.4112133042193257E-5	0.0	0.0	0.0320254085886323	0.0
2	4.4112133042193257E-5	0.0	0.0	0.11142724806458017	0.0
3	4.4112133042193257E-5	0.0	0.0	0.1562010631024063	0.0
4	4.4112133042193257E-5	0.0	0.0	0.2348971084496791	0.0
5	4.4112133042193257E-5	0.0	0.0	0.43865105097156976	0.0
6	4.4112133042193257E-5	0.0	0.0	0.5918966011601491	0.0
7	4.4112133042193257E-5	0.0	0.0	0.7179249652616952	0.0
8	4.4112133042193257E-5	0.0	0.0	1.0220340104545755	0.0
9	4.4112133042193257E-5	0.0	0.0	1.1548556430446195	0.0
10	4.4112133042193257E-5	0.0	0.0	1.4304682502922428	0.0
11	4.4112133042193257E-5	0.0	0.0	1.609475286177463	0.0
12	4.4112133042193257E-5	0.0	0.0	1.791570171375637	0.0
13	4.4112133042193257E-5	0.0	0.0	1.8562385584154921	0.0
14	4.4112133042193257E-5	0.0	0.0	1.8807649043869517	0.0
15	4.4112133042193257E-5	0.0	0.0	1.9208628333223052	0.0
16	4.4112133042193257E-5	0.0	0.0	1.9998676636008734	0.0
17	4.4112133042193257E-5	0.0	0.0	2.0907386576677913	0.0
18	4.4112133042193257E-5	0.0	0.0	2.23692626656962	0.0
19	4.4112133042193257E-5	0.0	0.0	2.3008888594808004	0.0
20	4.4112133042193257E-5	0.0	0.0	2.3678510774388495	0.0
21	4.4112133042193257E-5	0.0	0.0	2.450870111824257	0.0
22	4.4112133042193257E-5	0.0	0.0	2.5401530691016565	0.0
23	4.4112133042193257E-5	0.0	0.0	2.643640133218642	0.0
24	4.4112133042193257E-5	0.0	0.0	2.7155429100774167	0.0
25	4.4112133042193257E-5	0.0	0.0	2.7783585875295	0.0
26	4.4112133042193257E-5	0.0	0.0	2.8439533293632415	0.0
27	4.4112133042193257E-5	0.0	0.0	2.9069895674805357	0.0
28	4.4112133042193257E-5	0.0	0.0	2.977921877412382	0.0
29	4.4112133042193257E-5	0.0	0.0	3.0546769889057988	0.0
30	4.4112133042193257E-5	0.0	0.0	3.1557819978385053	0.0
31	4.4112133042193257E-5	0.0	0.0	3.2431681333950904	0.0
32	4.4112133042193257E-5	0.0	0.0	3.3281722137673966	0.0
33	4.4112133042193257E-5	0.0	0.0	3.4098678841615384	0.0
34	8.822426608438651E-5	0.0	0.0	3.4973422439842077	0.0
35	8.822426608438651E-5	0.0	0.0	3.606960894594058	0.0
36	8.822426608438651E-5	0.0	0.0	3.7018019806347735	0.0
37	8.822426608438651E-5	0.0	0.0	3.7930258717660292	0.0
38	8.822426608438651E-5	0.0	0.0	3.8880875184719557	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGTA	30	2.5288118E-6	44.0	23
CTCCGAT	20	7.858105E-4	44.0	22
CGTGATA	20	7.858105E-4	44.0	13
CATTGCG	30	2.5288118E-6	44.0	1
CACGCTA	25	4.4442146E-5	44.0	19
CGTCTAC	20	7.858105E-4	44.0	23
CCGCTTA	35	1.4469151E-7	44.0	26
CGCTCGA	35	1.4469151E-7	44.0	27
TATTACG	70	0.0	44.0	1
ACTCGAT	40	8.3182385E-9	44.0	20
TCGTACC	35	1.4469151E-7	44.0	15
ACGTAGA	25	4.4442146E-5	44.0	23
CCAACGG	25	4.4442146E-5	44.0	2
CGGTATA	20	7.858105E-4	44.0	23
TCGAATC	25	4.4442146E-5	44.0	37
GTACGAT	30	2.5288118E-6	44.0	25
GTTGCGA	25	4.4442146E-5	44.0	38
CTTACGA	20	7.858105E-4	44.0	28
CTATACG	95	0.0	41.68421	1
TCGTTAG	170	0.0	41.411762	1
>>END_MODULE