FastQCFastQC Report
Thu 26 May 2016
SRR1545902_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545902_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2995031
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195380.652347171030951No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA32560.10871339895981043No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAC207.858509E-444.028
ACTAGCG454.802132E-1044.01
GCGATCG408.3200575E-944.09
CGACGGT4000.041.828
CGTTTTT110100.041.0027281
CTATGCG1450.039.4482771
TAACGGG8100.039.3827173
AGGGCGA23750.039.183166
TAGACGC452.3530447E-839.1111142
GCGCGAC6900.038.579719
CACGACG4300.038.37209326
TCGATTG750.038.133331
CCGCTTA357.294124E-637.71428716
CTACGGG5950.037.7142873
CCCGCTA357.294124E-637.71428731
TACGGGT3050.037.5081984
TAGGCGG2950.037.2881362
CGACCCG7000.037.08571232
ACGTCGG950.037.052632
CGCCGTT950.037.0526326
Produced by FastQC (version 0.11.5)