##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545902_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2995031
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.742043404559084	31.0	31.0	33.0	30.0	34.0
2	32.18085388765592	33.0	31.0	34.0	30.0	34.0
3	32.25464711383622	34.0	31.0	34.0	30.0	34.0
4	35.93052926664198	37.0	35.0	37.0	35.0	37.0
5	35.91980550451731	37.0	35.0	37.0	35.0	37.0
6	35.969570264882066	37.0	35.0	37.0	35.0	37.0
7	36.14656041957495	37.0	35.0	37.0	35.0	37.0
8	36.108208228896466	37.0	35.0	37.0	35.0	37.0
9	37.93871782963181	39.0	39.0	39.0	35.0	39.0
10	37.61662567098638	39.0	37.0	39.0	35.0	39.0
11	37.37560045288346	39.0	37.0	39.0	35.0	39.0
12	36.757832222771654	39.0	35.0	39.0	33.0	39.0
13	36.54337601180088	39.0	35.0	39.0	33.0	39.0
14	37.592000216358365	40.0	35.0	41.0	33.0	41.0
15	37.777868743261756	40.0	35.0	41.0	33.0	41.0
16	37.83180240872298	40.0	35.0	41.0	33.0	41.0
17	37.773478471508305	40.0	35.0	41.0	33.0	41.0
18	37.7025643474141	39.0	36.0	41.0	33.0	41.0
19	37.65484797987066	39.0	36.0	41.0	34.0	41.0
20	37.51383441440172	39.0	35.0	41.0	33.0	41.0
21	37.399538769381685	39.0	35.0	41.0	33.0	41.0
22	37.40525891050877	39.0	35.0	41.0	33.0	41.0
23	37.38242275288636	39.0	35.0	41.0	33.0	41.0
24	37.34914964152291	39.0	35.0	41.0	33.0	41.0
25	37.27777608979673	39.0	35.0	41.0	33.0	41.0
26	37.1979972828328	39.0	35.0	41.0	33.0	41.0
27	37.11967288485495	39.0	35.0	41.0	33.0	41.0
28	37.12566247227491	39.0	35.0	41.0	33.0	41.0
29	37.09103411617443	39.0	35.0	41.0	33.0	41.0
30	36.977037299446984	39.0	35.0	41.0	32.0	41.0
31	36.841351558631615	39.0	35.0	41.0	32.0	41.0
32	36.70820569136012	39.0	35.0	41.0	32.0	41.0
33	36.56471335355126	39.0	35.0	41.0	31.0	41.0
34	36.424174240600514	39.0	35.0	41.0	31.0	41.0
35	36.23977180870582	38.0	35.0	41.0	30.0	41.0
36	36.115654896393394	38.0	35.0	41.0	30.0	41.0
37	36.05073236303731	38.0	35.0	41.0	30.0	41.0
38	35.958998421051405	38.0	35.0	41.0	30.0	41.0
39	35.9095775636379	38.0	35.0	41.0	30.0	41.0
40	35.756323390308815	38.0	35.0	41.0	29.0	41.0
41	35.66980108052304	38.0	35.0	40.0	29.0	41.0
42	35.63347791725695	38.0	35.0	40.0	29.0	41.0
43	35.57560906715156	38.0	35.0	40.0	29.0	41.0
44	35.479984347407424	38.0	35.0	40.0	28.0	41.0
45	35.38187451148252	38.0	35.0	40.0	28.0	41.0
46	35.34172467663941	38.0	35.0	40.0	28.0	41.0
47	35.27648795621815	38.0	35.0	40.0	28.0	41.0
48	35.21783013264304	37.0	34.0	40.0	28.0	41.0
49	35.1725344412128	37.0	34.0	40.0	27.0	41.0
50	35.023947999202676	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	2.0
12	7.0
13	14.0
14	25.0
15	57.0
16	140.0
17	388.0
18	845.0
19	1690.0
20	2985.0
21	4721.0
22	7289.0
23	11118.0
24	16690.0
25	25138.0
26	35156.0
27	43522.0
28	46435.0
29	49012.0
30	55197.0
31	65934.0
32	83195.0
33	112416.0
34	216335.0
35	433225.0
36	169728.0
37	226767.0
38	408746.0
39	978252.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.8374564403507	23.19431752125437	30.037886085319315	13.93033995307561
2	31.681642026409744	24.388962918914693	31.367187852145772	12.562207202529791
3	28.4825098638378	24.36619186913257	34.1805477138634	12.970750553166226
4	25.53439346704592	26.859755374819162	33.20429738456797	14.401553773566953
5	22.458932812381573	32.16253855135389	31.83783406582436	13.540694570440174
6	20.61003709143578	40.86071229312819	28.25249555012953	10.276755065306503
7	88.29534652562862	3.5673086522309787	6.783302076005223	1.3540427461351818
8	90.0916217561688	2.4584386605681208	6.1189683846344165	1.330971198628662
9	86.06311587425974	3.8578899517233713	7.654912419938224	2.424081754078672
10	46.410304267301406	25.765910269376175	15.728685279050532	12.095100184271883
11	39.086072898744625	21.846952502328023	25.012629251583707	14.05434534734365
12	35.349316918589494	23.175686662341725	26.127442420462426	15.347553998606358
13	21.844949184165372	34.61600230515143	27.06282505923979	16.47622345144341
14	17.038588248335326	36.33431507052848	29.929172686359507	16.69792399477668
15	16.740828392093437	24.736304899682175	42.67832286210059	15.844543846123797
16	18.952491643659116	21.429060333599217	41.00605302582845	18.61239499691322
17	19.25873221345622	21.26405369426894	30.0749140826923	29.40230000958254
18	21.78932371651579	23.894043166831995	34.721710726867265	19.594922389784948
19	27.054144013868303	24.668392413968338	28.283112929382032	19.994350642781328
20	27.91971101467731	24.931628420540555	27.864486210660257	19.284174354121877
21	22.7925520637349	27.08559610902191	31.377271220231112	18.744580607012082
22	22.382138949479987	24.23070078406534	28.993623104401923	24.39353716205275
23	19.306912015267955	28.78945159499184	29.37745886436568	22.52617752537453
24	19.680063411697578	25.218837467792486	38.176933727897975	16.92416539261196
25	18.763845850009567	26.26527071005275	35.35302305719039	19.617860382747292
26	18.402113367107052	32.26904830033479	29.26994745630346	20.058890876254704
27	17.39975312442509	32.26013353451099	31.25016068281096	19.089952658252955
28	15.58935450083822	28.380006751182208	37.702748318798704	18.327890429180865
29	17.086934993327283	24.835836423729837	37.15186921270598	20.9253593702369
30	18.33022763370396	29.83171125774658	33.38155765332646	18.456503455223
31	25.96293661067281	26.596652922791115	28.798967356264427	18.641443110271645
32	26.81985595474638	26.528272996172657	29.243236547468122	17.408634501612838
33	25.34865248473221	27.265560857299974	28.431892691594847	18.95389396637297
34	19.398396878028976	27.49687732781397	30.42275689300044	22.681968901156615
35	20.025235131122184	26.61885636576049	32.68353482818709	20.672373674930242
36	26.80857059576345	26.22754155132284	28.58023840153908	18.383649451374627
37	19.678494145803498	31.857166086093937	29.83000843730833	18.634331330794236
38	20.68252382028767	31.923142030917205	27.509965673143284	19.884368475651836
39	20.419221036443364	30.723788835574656	28.554662706329246	20.30232742165273
40	23.00156492537139	26.542897218760007	28.245483936560255	22.210053919308347
41	18.57136036321494	25.82280450519544	29.92406422504475	25.681770906544877
42	21.319612384646437	26.484567271590848	27.71163303485006	24.48418730891266
43	20.59007068708137	26.970772589666016	28.578902856097315	23.860253867155297
44	20.03725504009808	29.403101336847598	29.843664389450392	20.715979233603925
45	19.13813246006469	34.22435360435334	25.673123249809432	20.964390685772536
46	20.722055965363964	31.545750277709985	28.094700856184794	19.63749290074126
47	20.705395035977926	28.324882113073286	28.875861385074142	22.093861465874642
48	21.279379078213214	26.63361414289201	31.966146594142096	20.12086018475268
49	20.909432990843836	26.55735449816713	32.46443859846526	20.068773912523778
50	20.413411413771676	31.196805642412386	28.700904932202704	19.688878011613237
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2084.0
1	4253.5
2	6423.0
3	20581.5
4	34740.0
5	24255.5
6	13771.0
7	13538.0
8	13305.0
9	13487.5
10	13670.0
11	13380.5
12	13091.0
13	12195.0
14	11299.0
15	10623.5
16	9948.0
17	10224.0
18	10500.0
19	10022.0
20	9544.0
21	11583.0
22	13622.0
23	14021.5
24	14421.0
25	19379.5
26	24338.0
27	30547.5
28	36757.0
29	43017.0
30	49277.0
31	57487.5
32	65698.0
33	77050.0
34	88402.0
35	96050.5
36	103699.0
37	110446.5
38	117194.0
39	138974.0
40	160754.0
41	201610.0
42	242466.0
43	272540.0
44	302614.0
45	301126.0
46	299638.0
47	285899.5
48	272161.0
49	260153.0
50	248145.0
51	230640.0
52	213135.0
53	190949.0
54	168763.0
55	151562.0
56	134361.0
57	118477.0
58	102593.0
59	89649.0
60	76705.0
61	63029.5
62	49354.0
63	39783.5
64	30213.0
65	23407.5
66	16602.0
67	13245.0
68	9888.0
69	8196.5
70	6505.0
71	5250.0
72	3995.0
73	3220.5
74	2446.0
75	1889.0
76	1332.0
77	1032.0
78	732.0
79	530.5
80	329.0
81	267.5
82	206.0
83	168.0
84	130.0
85	96.0
86	62.0
87	57.5
88	53.0
89	39.5
90	26.0
91	29.0
92	32.0
93	17.5
94	3.0
95	3.5
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2995031.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.281376091282265
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.14614405899579	10.511697054223285
2	6.941113155571678	1.84375068622589
3	2.3052798275428863	0.918518651557302
4	1.2063382142546457	0.6408732606720717
5	0.7956173304037399	0.5283446494917028
6	0.5931130121941453	0.47264141877502364
7	0.442413429092409	0.41131013977270864
8	0.3770120336299479	0.4005790887662796
9	0.3127555934200249	0.3738442194777164
>10	4.01369305979122	12.576691744003407
>50	1.503485434155076	14.577558927067995
>100	2.3526048338609056	55.001647276618556
>500	0.007631719820135915	0.6222812375279716
>1k	0.0025439066067119718	0.4601394382611275
>5k	0.0	0.0
>10k+	2.543906606711972E-4	0.6601222075589918
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19538	0.652347171030951	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3256	0.10871339895981043	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01669431802208391	0.0
2	0.0	0.0	0.0	0.05615968582629028	0.0
3	0.0	0.0	0.0	0.0797988401455611	0.0
4	0.0	0.0	0.0	0.11485690799193732	0.0
5	0.0	0.0	0.0	0.20126669807424363	0.0
6	0.0	0.0	0.0	0.2906814654005251	0.0
7	0.0	0.0	0.0	0.352350276174103	0.0
8	0.0	0.0	0.0	0.5028328588251674	0.0
9	0.0	0.0	0.0	0.569009135464708	0.0
10	0.0	0.0	0.0	0.6788911366860644	0.0
11	0.0	0.0	0.0	0.7804259788963787	0.0
12	0.0	0.0	0.0	0.8754834257141245	0.0
13	0.0	0.0	0.0	0.9109421571930307	0.0
14	0.0	0.0	0.0	0.9239637252502562	0.0
15	0.0	0.0	0.0	0.9424944182547693	0.0
16	0.0	0.0	0.0	0.9874689110062633	0.0
17	0.0	0.0	0.0	1.042326440026831	0.0
18	0.0	0.0	0.0	1.1253973665047206	0.0
19	0.0	0.0	0.0	1.1639278524996903	0.0
20	0.0	0.0	0.0	1.2116736020428502	0.0
21	0.0	0.0	0.0	1.2707380992049833	0.0
22	0.0	0.0	0.0	1.3343768395051672	0.0
23	0.0	0.0	0.0	1.4024562683992252	0.0
24	0.0	0.0	0.0	1.4549765928967013	0.0
25	0.0	0.0	0.0	1.5025887878956845	0.0
26	0.0	0.0	0.0	1.5477970010994877	0.0
27	0.0	0.0	0.0	1.5975126801692536	0.0
28	0.0	0.0	0.0	1.651669047832894	0.0
29	0.0	0.0	0.0	1.7084965063800674	0.0
30	0.0	0.0	0.0	1.7808830693238233	0.0
31	0.0	0.0	0.0	1.8447555300763163	0.0
32	0.0	0.0	0.0	1.9084276590125444	0.0
33	0.0	0.0	0.0	1.9729678924859209	0.0
34	0.0	0.0	0.0	2.0416483168287742	0.0
35	0.0	0.0	0.0	2.1292267091726265	0.0
36	0.0	0.0	0.0	2.199409622137467	0.0
37	0.0	0.0	0.0	2.2780732486575266	0.0
38	0.0	0.0	0.0	2.3792074272353108	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTAC	20	7.858509E-4	44.0	28
ACTAGCG	45	4.802132E-10	44.0	1
GCGATCG	40	8.3200575E-9	44.0	9
CGACGGT	400	0.0	41.8	28
CGTTTTT	11010	0.0	41.002728	1
CTATGCG	145	0.0	39.448277	1
TAACGGG	810	0.0	39.382717	3
AGGGCGA	2375	0.0	39.18316	6
TAGACGC	45	2.3530447E-8	39.11111	42
GCGCGAC	690	0.0	38.57971	9
CACGACG	430	0.0	38.372093	26
TCGATTG	75	0.0	38.13333	1
CCGCTTA	35	7.294124E-6	37.714287	16
CTACGGG	595	0.0	37.714287	3
CCCGCTA	35	7.294124E-6	37.714287	31
TACGGGT	305	0.0	37.508198	4
TAGGCGG	295	0.0	37.288136	2
CGACCCG	700	0.0	37.085712	32
ACGTCGG	95	0.0	37.05263	2
CGCCGTT	95	0.0	37.05263	26
>>END_MODULE