FastQCFastQC Report
Thu 26 May 2016
SRR1545901_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545901_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3251246
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT323970.9964487461114908No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA33060.10168409280626567No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTTA207.8586076E-444.00000443
TACGCAC454.802132E-1044.010
GTCGTAC454.802132E-1044.010
ATACCCG351.4471698E-744.031
CGGTCTA4800.042.1666731
CGTTTTT158200.041.858411
ACGTAGG3500.040.8571432
ATAGCGG5800.039.4482772
ATACCGG1900.039.368422
CACGACG5100.039.25489826
CGACGGT5250.038.97142828
ACGGTCT5150.038.87378730
ACGGGAC6350.038.8031465
ATATCGC404.1276326E-738.50000424
CATATGC21000.038.44761733
TCGCCGT1550.038.3225825
AGGGCGA24750.038.222226
GTTGATC21500.037.86046616
TTACGCA357.2943167E-637.71428732
GGGCGAT43750.037.6639987
Produced by FastQC (version 0.11.5)