##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545901_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3251246
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.728405663551758	31.0	31.0	33.0	30.0	34.0
2	32.15295397518367	33.0	31.0	34.0	30.0	34.0
3	32.196898050778074	33.0	31.0	34.0	30.0	34.0
4	35.90582472073783	37.0	35.0	37.0	35.0	37.0
5	35.89914143685221	37.0	35.0	37.0	35.0	37.0
6	35.95608975758832	37.0	35.0	37.0	35.0	37.0
7	36.13002861056961	37.0	35.0	37.0	35.0	37.0
8	36.0835464311221	37.0	35.0	37.0	35.0	37.0
9	37.907622800612444	39.0	39.0	39.0	35.0	39.0
10	37.610433353858795	39.0	37.0	39.0	35.0	39.0
11	37.365979996592074	39.0	37.0	39.0	35.0	39.0
12	36.73983143693218	39.0	35.0	39.0	33.0	39.0
13	36.53500657901617	39.0	35.0	39.0	33.0	39.0
14	37.54792654877546	40.0	35.0	41.0	33.0	41.0
15	37.73016929509487	40.0	35.0	41.0	33.0	41.0
16	37.793472410269786	40.0	35.0	41.0	33.0	41.0
17	37.728031960669846	40.0	35.0	41.0	33.0	41.0
18	37.64497672584603	39.0	36.0	41.0	33.0	41.0
19	37.55861937238831	39.0	36.0	41.0	33.0	41.0
20	37.38987145236011	39.0	35.0	41.0	33.0	41.0
21	37.266048462650936	39.0	35.0	41.0	33.0	41.0
22	37.27924955540122	39.0	35.0	41.0	33.0	41.0
23	37.269441930878195	39.0	35.0	41.0	33.0	41.0
24	37.24044504783704	39.0	35.0	41.0	33.0	41.0
25	37.144647313676046	39.0	35.0	41.0	33.0	41.0
26	37.04776722524226	39.0	35.0	41.0	33.0	41.0
27	36.96206131433918	39.0	35.0	41.0	33.0	41.0
28	36.93974310156783	38.0	35.0	41.0	33.0	41.0
29	36.864002908423416	38.0	35.0	41.0	32.0	41.0
30	36.73017698445457	38.0	35.0	41.0	32.0	41.0
31	36.53745948476369	38.0	35.0	41.0	31.0	41.0
32	36.37570795934851	38.0	35.0	41.0	31.0	41.0
33	36.18237869419909	38.0	35.0	41.0	30.0	41.0
34	35.98428294875257	38.0	35.0	41.0	30.0	41.0
35	35.765312437139485	38.0	35.0	41.0	29.0	41.0
36	35.627472974976364	38.0	35.0	41.0	29.0	41.0
37	35.54802374228219	38.0	35.0	41.0	28.0	41.0
38	35.45605131078977	38.0	35.0	41.0	28.0	41.0
39	35.3798509863603	38.0	35.0	40.0	27.0	41.0
40	35.20922901558357	38.0	35.0	40.0	26.0	41.0
41	35.08115780842176	38.0	34.0	40.0	25.0	41.0
42	35.06954626011074	38.0	34.0	40.0	26.0	41.0
43	35.00285244487806	37.0	34.0	40.0	25.0	41.0
44	34.849979361758535	37.0	34.0	40.0	24.0	41.0
45	34.760826772259	37.0	34.0	40.0	24.0	41.0
46	34.7155988811674	37.0	34.0	40.0	24.0	41.0
47	34.64695719733296	37.0	34.0	40.0	24.0	41.0
48	34.58149244935634	36.0	34.0	40.0	24.0	41.0
49	34.50987098484704	36.0	34.0	40.0	24.0	41.0
50	34.360032123069125	36.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	3.0
10	3.0
11	7.0
12	15.0
13	20.0
14	41.0
15	99.0
16	228.0
17	478.0
18	1149.0
19	2275.0
20	3841.0
21	6228.0
22	9789.0
23	14347.0
24	21555.0
25	32424.0
26	46397.0
27	56439.0
28	58698.0
29	59733.0
30	65738.0
31	77508.0
32	95258.0
33	126058.0
34	241092.0
35	469485.0
36	191632.0
37	256256.0
38	443343.0
39	971104.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.98329317437069	22.24088857010512	29.927510868141017	14.848307387383175
2	32.94346229107241	24.127488353695785	30.352609430353777	12.57643992487803
3	28.556067427687726	24.03589270082916	34.364548237814056	13.04349163366906
4	25.53919328159112	26.798987219053867	33.35284380203774	14.308975697317274
5	23.020312827758957	31.11760844919148	32.044699170717934	13.81737955233163
6	20.595396349584128	40.36187972242027	28.683187922414977	10.35953600558063
7	86.41809939943025	3.797067339721448	8.35030631333341	1.4345269475148912
8	87.78702072989863	2.7164969983815435	7.991889878526572	1.5045923931932559
9	83.84050299485182	4.188917110547771	9.458650621946171	2.5119292726542377
10	46.9585199028311	23.05285419805207	17.371001763631543	12.61762413548529
11	39.26848352908392	23.030770356964684	23.957553504102734	13.743192609848656
12	35.87778347132146	21.83581310057744	27.229960452085137	15.056442976015965
13	21.839227176288723	32.997872200381025	27.66647002410768	17.496430599222577
14	17.35491562311803	34.707585953200706	31.338077770799256	16.599420652882003
15	17.27288553373076	23.108771221863865	42.62959493068196	16.988748313723416
16	19.12965060164626	19.89514173950541	41.10867033746447	19.866537321383863
17	19.903661550064193	20.091343441868133	30.86453624241291	29.14045876565477
18	22.48055053354929	22.708001793773832	34.807086267849314	20.004361404827563
19	27.106346305385685	24.391787025651087	28.503810539097934	19.998056129865287
20	28.85044687482891	23.30854078713207	28.66848586664928	19.172526471389737
21	23.094776587191497	26.787268634855682	31.19557855665182	18.922376221301
22	22.690531568512505	23.59138619470812	29.561466588501762	24.15661564827761
23	20.112412287473788	27.387838385652763	29.056952319203162	23.44279700767029
24	19.776418025581577	23.86272832015787	38.957249005458216	17.403604648802336
25	19.351380978246493	24.749311494731558	35.4827041694169	20.416603357605055
26	18.37950742576846	30.331356040115082	29.86922552153851	21.41991101257795
27	17.76820332881609	30.786873709340973	31.43302598449948	20.011896977343454
28	17.257506814310574	27.042924466496842	36.629649063774316	19.06991965541826
29	17.914670252573934	24.921645424554157	35.65393083144124	21.50975349143067
30	20.43468873164319	28.68423367533555	32.798902328522665	18.082175264498595
31	26.51488075648536	26.22797536698238	29.09112383375481	18.166020042777443
32	28.42906996271583	24.27334012867682	28.99525289688938	18.30233701171797
33	26.73648810333023	26.249197999782236	27.417119467428797	19.597194429458735
34	20.452958650314372	26.038540301164538	30.703121203378643	22.805379845142447
35	20.698526042015892	26.07606437654979	32.12251549098407	21.102894090450246
36	27.45147552661349	26.548652424332086	26.99032924607981	19.009542802974615
37	20.65555789995589	31.698062834986956	28.927678803757082	18.718700461300067
38	21.85682043130541	30.636931194994165	26.92223227648723	20.584016097213194
39	21.60953062302883	30.240314021147586	27.628884433844746	20.521270921978836
40	24.255131724883324	26.08984370915028	27.98351770367422	21.67150686229218
41	19.41830916516314	24.97682426983378	29.788179670194136	25.816686894808942
42	21.57625107420355	25.421822894976266	27.610337698224	25.391588332596182
43	21.320933574389635	25.70445300048043	28.56172679643435	24.41288662869558
44	20.610190677666345	27.613321169791522	29.863135548648117	21.913352603894015
45	19.414618272502295	33.31193640838005	26.150189804155083	21.123255514962572
46	21.40843848789049	30.498614992529017	27.985086333055083	20.10786018652541
47	20.39574981407128	28.341257474826577	29.245556934172313	22.01743577692983
48	21.78029592347057	26.57405806881423	31.38627467746212	20.259371330253078
49	21.524916908778973	26.277986962536826	31.972634491514945	20.22446163716926
50	20.667399513909437	30.281775048704407	29.060274122597924	19.990551314788238
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3174.0
1	6438.5
2	9703.0
3	32661.0
4	55619.0
5	37848.0
6	20077.0
7	19702.0
8	19327.0
9	19259.5
10	19192.0
11	18644.0
12	18096.0
13	16770.0
14	15444.0
15	14606.0
16	13768.0
17	13260.0
18	12752.0
19	12575.0
20	12398.0
21	12434.5
22	12471.0
23	13323.5
24	14176.0
25	17670.0
26	21164.0
27	26406.5
28	31649.0
29	38981.0
30	46313.0
31	52505.0
32	58697.0
33	68794.0
34	78891.0
35	88495.5
36	98100.0
37	109333.0
38	120566.0
39	135850.5
40	151135.0
41	189582.0
42	228029.0
43	261074.0
44	294119.0
45	291887.0
46	289655.0
47	282257.5
48	274860.0
49	263963.0
50	253066.0
51	241848.0
52	230630.0
53	209112.0
54	187594.0
55	179006.5
56	170419.0
57	156694.5
58	142970.0
59	126882.5
60	110795.0
61	97285.5
62	83776.0
63	70362.0
64	56948.0
65	44984.5
66	33021.0
67	27135.5
68	21250.0
69	18376.0
70	15502.0
71	13369.5
72	11237.0
73	8925.0
74	6613.0
75	5062.5
76	3512.0
77	2895.0
78	2278.0
79	1665.5
80	1053.0
81	813.0
82	573.0
83	440.0
84	307.0
85	239.5
86	172.0
87	121.0
88	70.0
89	64.0
90	58.0
91	36.0
92	14.0
93	10.5
94	7.0
95	6.0
96	5.0
97	2.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3251246.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.459419322212213
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.65151853816366	11.372552868720557
2	6.895165458183686	1.9940017731182282
3	2.3652260051018192	1.0259938379870417
4	1.3634152875050547	0.7885677340940044
5	0.9105152355694011	0.6582760795180401
6	0.6756532325963219	0.5861732043911041
7	0.4986754178133118	0.5047389880289435
8	0.42624925157373744	0.4930653331427035
9	0.35626059919067277	0.4636189252512463
>10	4.546686259378742	15.604340875038794
>50	1.348458630919528	14.08134384909049
>100	1.948376166428929	49.772587578150855
>500	0.010781185605535332	0.9760253081495325
>1k	0.002803108257439186	0.6686420303010661
>5k	0.0	0.0
>10k+	2.1562371211070663E-4	1.010071615017422
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32397	0.9964487461114908	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3306	0.10168409280626567	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.013133426384838305	0.0
2	0.0	0.0	0.0	0.04982705092140059	0.0
3	0.0	0.0	0.0	0.0750481507705046	0.0
4	0.0	0.0	0.0	0.10998860129316576	0.0
5	0.0	0.0	0.0	0.18792795131466522	0.0
6	0.0	0.0	0.0	0.2831837394032934	0.0
7	0.0	0.0	0.0	0.3438374087965045	0.0
8	0.0	0.0	0.0	0.5029763973565827	0.0
9	0.0	0.0	0.0	0.5739953236389987	0.0
10	0.0	0.0	0.0	0.6882899663698163	0.0
11	0.0	0.0	0.0	0.8075057993150934	0.0
12	0.0	0.0	0.0	0.901469774972426	0.0
13	0.0	0.0	0.0	0.9392091524295608	0.0
14	0.0	0.0	0.0	0.954987718554671	0.0
15	0.0	0.0	0.0	0.9759642918438038	0.0
16	0.0	0.0	0.0	1.0225310542481252	0.0
17	0.0	0.0	0.0	1.0754953639312437	0.0
18	0.0	0.0	0.0	1.1579560574622776	0.0
19	0.0	0.0	0.0	1.2003398081843084	0.0
20	0.0	0.0	0.0	1.2487212594802115	0.0
21	0.0	0.0	0.0	1.3105129541105165	0.0
22	0.0	0.0	0.0	1.3727967677622672	0.0
23	0.0	0.0	0.0	1.4437849366058428	0.0
24	0.0	0.0	0.0	1.5012705897984957	0.0
25	0.0	0.0	0.0	1.5473144757425308	0.0
26	0.0	0.0	0.0	1.5959112291103166	0.0
27	0.0	0.0	0.0	1.6525971888931197	0.0
28	0.0	0.0	0.0	1.709436935870125	0.0
29	0.0	0.0	0.0	1.7758730037653256	0.0
30	0.0	0.0	0.0	1.8518438777010413	0.0
31	0.0	0.0	0.0	1.9140969339139517	0.0
32	0.0	0.0	0.0	1.9743815140410783	0.0
33	0.0	0.0	0.0	2.037834110368763	0.0
34	0.0	0.0	0.0	2.1069768328819167	0.0
35	3.075743884037074E-5	0.0	0.0	2.197865064655212	0.0
36	3.075743884037074E-5	0.0	0.0	2.2668539999741637	0.0
37	3.075743884037074E-5	0.0	0.0	2.3446703202403016	0.0
38	3.075743884037074E-5	0.0	0.0	2.43660430493417	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCGTTA	20	7.8586076E-4	44.000004	43
TACGCAC	45	4.802132E-10	44.0	10
GTCGTAC	45	4.802132E-10	44.0	10
ATACCCG	35	1.4471698E-7	44.0	31
CGGTCTA	480	0.0	42.16667	31
CGTTTTT	15820	0.0	41.85841	1
ACGTAGG	350	0.0	40.857143	2
ATAGCGG	580	0.0	39.448277	2
ATACCGG	190	0.0	39.36842	2
CACGACG	510	0.0	39.254898	26
CGACGGT	525	0.0	38.971428	28
ACGGTCT	515	0.0	38.873787	30
ACGGGAC	635	0.0	38.803146	5
ATATCGC	40	4.1276326E-7	38.500004	24
CATATGC	2100	0.0	38.447617	33
TCGCCGT	155	0.0	38.32258	25
AGGGCGA	2475	0.0	38.22222	6
GTTGATC	2150	0.0	37.860466	16
TTACGCA	35	7.2943167E-6	37.714287	32
GGGCGAT	4375	0.0	37.663998	7
>>END_MODULE