FastQCFastQC Report
Thu 26 May 2016
SRR1545900_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545900_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2453903
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT378221.5412997172259866No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA33750.13753599877419767No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGAT454.802132E-1044.029
CGTTTTT177650.042.2043341
TCGATAG1200.040.3333321
TACGGGA7250.040.0551724
CGGATAC452.3528628E-839.111116
ACGGGAT7550.038.4635775
TACACCG357.2935854E-637.71428731
CATACGA8050.037.71428318
TAGCCGT651.0913936E-1137.2307744
CTAGCGG3250.037.230772
CGTTAGG1900.037.052632
CGTTCGA1250.036.9614
TGTTACG2500.036.961
TTACGGG8850.036.790963
AGGGCGA20400.036.774516
ACGTTAC601.9826984E-1036.66666440
CTATCGA301.3015258E-436.66666415
GCGCGAC6500.036.5538449
CGACGGT2000.036.30000328
GGGCGAT35700.036.296927
Produced by FastQC (version 0.11.5)