##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545900_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2453903
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.688891940716484	31.0	31.0	33.0	30.0	34.0
2	32.10710855319057	33.0	31.0	34.0	30.0	34.0
3	32.04791265180408	33.0	31.0	34.0	30.0	34.0
4	35.81105406366918	37.0	35.0	37.0	35.0	37.0
5	35.842147794757985	37.0	35.0	37.0	35.0	37.0
6	35.89541314387733	37.0	35.0	37.0	35.0	37.0
7	36.08183208545733	37.0	35.0	37.0	35.0	37.0
8	36.02301557967043	37.0	35.0	37.0	35.0	37.0
9	37.739882546294616	39.0	38.0	39.0	35.0	39.0
10	37.53967088348643	39.0	37.0	39.0	35.0	39.0
11	37.36378740316956	39.0	37.0	39.0	35.0	39.0
12	36.85699353234419	39.0	35.0	39.0	33.0	39.0
13	36.6888903106602	39.0	35.0	39.0	33.0	39.0
14	37.79635625369055	40.0	35.0	41.0	33.0	41.0
15	37.91678032913281	40.0	35.0	41.0	33.0	41.0
16	37.951976911882824	40.0	35.0	41.0	33.0	41.0
17	37.86760438371036	40.0	35.0	41.0	33.0	41.0
18	37.71747253253287	39.0	36.0	41.0	33.0	41.0
19	37.586776249916966	39.0	36.0	41.0	33.0	41.0
20	37.39073712367604	39.0	35.0	41.0	33.0	41.0
21	37.26337960383927	39.0	35.0	41.0	33.0	41.0
22	37.27027963208	39.0	35.0	41.0	33.0	41.0
23	37.24372601524999	39.0	35.0	41.0	33.0	41.0
24	37.19346852748458	39.0	35.0	41.0	33.0	41.0
25	37.09801446919459	39.0	35.0	41.0	33.0	41.0
26	37.00271282116693	39.0	35.0	41.0	33.0	41.0
27	36.899304495735976	39.0	35.0	41.0	32.0	41.0
28	36.816921858769476	38.0	35.0	41.0	32.0	41.0
29	36.71908302813925	38.0	35.0	41.0	32.0	41.0
30	36.58496158976129	38.0	35.0	41.0	31.0	41.0
31	36.3828818824542	38.0	35.0	41.0	31.0	41.0
32	36.148713702212355	38.0	35.0	41.0	30.0	41.0
33	35.900651329738785	38.0	35.0	41.0	30.0	41.0
34	35.62510376327019	38.0	35.0	41.0	27.0	41.0
35	35.35409060586339	38.0	35.0	41.0	26.0	41.0
36	35.19570822481573	38.0	35.0	41.0	24.0	41.0
37	35.11230598764499	38.0	35.0	41.0	24.0	41.0
38	35.009427838019676	38.0	34.0	40.0	24.0	41.0
39	34.938471080560234	38.0	34.0	40.0	23.0	41.0
40	34.78894072015071	38.0	34.0	40.0	23.0	41.0
41	34.63276380525229	38.0	34.0	40.0	23.0	41.0
42	34.59551335158725	38.0	34.0	40.0	23.0	41.0
43	34.5145431583889	37.0	34.0	40.0	23.0	41.0
44	34.377657144557055	37.0	34.0	40.0	22.0	41.0
45	34.27387879635014	37.0	34.0	40.0	22.0	41.0
46	34.21688999116917	37.0	34.0	40.0	22.0	41.0
47	34.136990744947944	37.0	34.0	40.0	22.0	41.0
48	34.04631886427459	36.0	34.0	40.0	21.0	41.0
49	33.97821837293487	36.0	34.0	40.0	20.0	41.0
50	33.83624617598984	36.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	9.0
12	9.0
13	19.0
14	51.0
15	86.0
16	203.0
17	485.0
18	1007.0
19	1995.0
20	3534.0
21	5780.0
22	8864.0
23	13325.0
24	19624.0
25	29921.0
26	43425.0
27	51867.0
28	52460.0
29	51376.0
30	54017.0
31	60668.0
32	72571.0
33	93675.0
34	167761.0
35	309007.0
36	151270.0
37	197158.0
38	338213.0
39	725520.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.13032218469923	20.64454055437399	27.47789949317475	17.747237767752026
2	37.82292943119593	23.264000247768553	26.67085862807128	12.24221169296423
3	29.09768642036788	23.95889324068637	34.23452353251127	12.708896806434483
4	25.674690482875643	25.99291822048386	33.64688824293381	14.685503053706686
5	23.069860544609956	30.02050203288394	32.671421812516634	14.238215609989474
6	20.906042333376664	38.0190659532997	31.156936521125733	9.917955192197898
7	81.31617264415098	4.461382540385663	11.982054710394014	2.2403901050693524
8	81.77291441430245	3.858506224573669	12.02512894763974	2.3434504134841516
9	76.27815769408978	5.739957936397649	14.237278327627456	3.744606041885111
10	41.72601769507597	25.138320463359797	21.025403204609148	12.110258636955088
11	35.08618718832815	23.715118323747923	27.799591100381722	13.399103387542214
12	31.51705670517539	22.72506288960892	31.07115480929768	14.686725595918013
13	21.646250890927636	31.35800396348185	31.184362218066486	15.811382927524031
14	17.32220059228095	32.74819746338792	34.04682255166565	15.88277939266548
15	17.081400528056733	23.353653343265808	43.786123575381744	15.77882255329571
16	19.46926997521907	21.668297402138553	40.75226282375465	18.110169798887732
17	19.310013476490308	21.461402508575116	33.25640826063621	25.972175754298355
18	22.01313580854663	22.81659054983021	36.191487601588165	18.978786040034997
19	26.049725681903478	23.89760312449188	31.069484001608867	18.98318719199577
20	27.04516030177232	23.733741716767128	31.16329374062463	18.057804240835925
21	23.0864056158699	25.7991860313957	33.23582880007889	17.878579552655506
22	22.450072394874613	23.84315924468082	31.69143197591755	22.015336384527018
23	19.94276057366571	26.466368067523447	31.46990732722524	22.1209640315856
24	19.852822218319144	23.920301658215504	38.6159518122762	17.610924311189155
25	19.1764303641994	25.176015514875687	36.2812221998995	19.366331921025402
26	18.33817392130007	29.009337369896038	32.128450065059624	20.524038643744273
27	18.04855367143689	29.459681169141565	33.061820292000135	19.42994486742141
28	17.33768612695775	26.95196183386222	37.35159865732264	18.358753381857394
29	18.378558565680876	24.473665014468786	37.03919837092175	20.108578048928585
30	19.46645812813302	27.416242614316864	34.93414368864621	18.183155568903906
31	25.137790695068222	26.2029509723897	30.612416220201045	18.046842112341032
32	25.767318431087126	26.03126529451246	31.02274213772916	17.178674136671255
33	24.74348008050848	26.582917091669884	29.894009665418725	18.779593162402914
34	20.083678939224576	26.949312992404344	31.967278250199783	20.999729818171296
35	20.48895168227921	26.721757135469492	32.48461736262599	20.304673819625307
36	25.03497489509569	27.252177449556893	29.496805700958838	18.216041954388583
37	20.37456248270612	30.61583933839276	30.242678704088956	18.766919474812166
38	20.546044403548144	30.450307122979186	28.99792697592366	20.005721497549008
39	20.48296122544371	29.447170487178997	29.78528491142478	20.284583375952515
40	22.635409794111666	26.85362868866455	29.4443178886859	21.066643628537886
41	19.120926947805188	26.34199477322453	30.485027321780855	24.052050957189426
42	20.835949913260627	27.241745089353575	28.62419582192124	23.298109175464557
43	20.902415458149733	27.08835679323918	29.12335980680573	22.88586794180536
44	20.031557889615033	28.51445228275119	30.191372682620297	21.262617145013472
45	18.919003725901145	32.635968088388175	27.949148764233954	20.495879421476726
46	21.248272649733913	30.044871374296378	29.61694084892516	19.08991512704455
47	20.459936680463734	28.765317944515328	30.017364174541537	20.7573812004794
48	21.27280499677453	27.09512152680852	31.500878396578834	20.13119507983812
49	20.969696031179716	26.765075881157486	32.39072612079614	19.87450196686666
50	19.668014587373666	30.338485262049886	30.2582049901728	19.735295160403652
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2984.0
1	5369.0
2	7754.0
3	35526.5
4	63299.0
5	42664.0
6	22029.0
7	21114.0
8	20199.0
9	20172.0
10	20145.0
11	19323.0
12	18501.0
13	17609.5
14	16718.0
15	15713.5
16	14709.0
17	14040.5
18	13372.0
19	12834.0
20	12296.0
21	13346.5
22	14397.0
23	16003.5
24	17610.0
25	19967.0
26	22324.0
27	25650.5
28	28977.0
29	36122.0
30	43267.0
31	50115.5
32	56964.0
33	62713.5
34	68463.0
35	78006.5
36	87550.0
37	90773.0
38	93996.0
39	107680.5
40	121365.0
41	146281.5
42	171198.0
43	183760.5
44	196323.0
45	198664.5
46	201006.0
47	195031.5
48	189057.0
49	181800.5
50	174544.0
51	169528.5
52	164513.0
53	148036.0
54	131559.0
55	119953.0
56	108347.0
57	100653.0
58	92959.0
59	86102.0
60	79245.0
61	69175.5
62	59106.0
63	50794.0
64	42482.0
65	33477.5
66	24473.0
67	21288.5
68	18104.0
69	15374.5
70	12645.0
71	10889.0
72	9133.0
73	7316.5
74	5500.0
75	4297.5
76	3095.0
77	2486.5
78	1878.0
79	1367.5
80	857.0
81	674.0
82	491.0
83	345.5
84	200.0
85	167.5
86	135.0
87	99.5
88	64.0
89	48.0
90	32.0
91	26.0
92	20.0
93	14.5
94	9.0
95	8.5
96	8.0
97	4.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2453903.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.560634835041824
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.12995803517747	14.501416207680709
2	6.050989806071366	2.2462042436210234
3	2.4309648003789976	1.3536074987002478
4	1.4853486169942547	1.1027605313105906
5	1.0119201041217942	0.9390939767421064
6	0.7532602518605295	0.8388593082320955
7	0.644016419915345	0.8367347518473773
8	0.5407359747425818	0.8029122375493969
9	0.4395058148463525	0.7341756243516587
>10	5.616289300904079	23.93382759780401
>50	1.906430526314895	25.663952729787475
>100	0.9859067517386818	24.707646857584688
>500	0.0033382835240437	0.40935343674947455
>1k	0.0011127611746812333	0.3671375727375711
>5k	0.0	0.0
>10k+	2.2255223493624667E-4	1.5623174253015768
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37822	1.5412997172259866	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3375	0.13753599877419767	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.075140704420672E-5	0.0	0.0	0.014018484023207111	0.0
2	4.075140704420672E-5	0.0	0.0	0.06389820624531614	0.0
3	4.075140704420672E-5	0.0	0.0	0.11137359545181696	0.0
4	4.075140704420672E-5	0.0	0.0	0.15493684958207393	0.0
5	4.075140704420672E-5	0.0	0.0	0.276579799609031	0.0
6	4.075140704420672E-5	0.0	0.0	0.4177834250172073	0.0
7	4.075140704420672E-5	0.0	0.0	0.5075180233285504	0.0
8	4.075140704420672E-5	0.0	0.0	0.6765956111549641	0.0
9	4.075140704420672E-5	0.0	0.0	0.7653114242902022	0.0
10	4.075140704420672E-5	0.0	0.0	0.9106309418098434	0.0
11	4.075140704420672E-5	0.0	0.0	1.0579472782746506	0.0
12	4.075140704420672E-5	0.0	0.0	1.1697691392039538	0.0
13	4.075140704420672E-5	0.0	0.0	1.2231127310248204	0.0
14	4.075140704420672E-5	0.0	0.0	1.2495196427894664	0.0
15	4.075140704420672E-5	0.0	0.0	1.2795126783740025	0.0
16	4.075140704420672E-5	0.0	0.0	1.341332562860064	0.0
17	4.075140704420672E-5	0.0	0.0	1.4064533113167066	0.0
18	4.075140704420672E-5	0.0	0.0	1.5040936825946258	0.0
19	4.075140704420672E-5	0.0	0.0	1.552628608384276	0.0
20	4.075140704420672E-5	0.0	0.0	1.609802832467298	0.0
21	4.075140704420672E-5	0.0	0.0	1.673864044340791	0.0
22	4.075140704420672E-5	0.0	0.0	1.7370287252593113	0.0
23	4.075140704420672E-5	0.0	0.0	1.8110332804515907	0.0
24	4.075140704420672E-5	0.0	0.0	1.868248255941657	0.0
25	4.075140704420672E-5	0.0	0.0	1.9182094809778545	0.0
26	4.075140704420672E-5	0.0	0.0	1.9687004743056267	0.0
27	4.075140704420672E-5	0.0	0.0	2.02143279502083	0.0
28	4.075140704420672E-5	0.0	0.0	2.0761211832741555	0.0
29	8.150281408841344E-5	0.0	0.0	2.1353329777093877	0.0
30	8.150281408841344E-5	0.0	0.0	2.2201774071754263	0.0
31	8.150281408841344E-5	0.0	0.0	2.288517516788561	0.0
32	8.150281408841344E-5	0.0	0.0	2.3566131179594305	0.0
33	8.150281408841344E-5	0.0	0.0	2.421733866416073	0.0
34	8.150281408841344E-5	0.0	0.0	2.4905222415066937	0.0
35	8.150281408841344E-5	0.0	0.0	2.5717397957457977	0.0
36	8.150281408841344E-5	0.0	0.0	2.6387351089264737	0.0
37	8.150281408841344E-5	0.0	0.0	2.713880703515991	0.0
38	8.150281408841344E-5	0.0	0.0	2.7960355401171113	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCGAT	45	4.802132E-10	44.0	29
CGTTTTT	17765	0.0	42.204334	1
TCGATAG	120	0.0	40.333332	1
TACGGGA	725	0.0	40.055172	4
CGGATAC	45	2.3528628E-8	39.11111	6
ACGGGAT	755	0.0	38.463577	5
TACACCG	35	7.2935854E-6	37.714287	31
CATACGA	805	0.0	37.714283	18
TAGCCGT	65	1.0913936E-11	37.23077	44
CTAGCGG	325	0.0	37.23077	2
CGTTAGG	190	0.0	37.05263	2
CGTTCGA	125	0.0	36.96	14
TGTTACG	250	0.0	36.96	1
TTACGGG	885	0.0	36.79096	3
AGGGCGA	2040	0.0	36.77451	6
ACGTTAC	60	1.9826984E-10	36.666664	40
CTATCGA	30	1.3015258E-4	36.666664	15
GCGCGAC	650	0.0	36.553844	9
CGACGGT	200	0.0	36.300003	28
GGGCGAT	3570	0.0	36.29692	7
>>END_MODULE