FastQCFastQC Report
Thu 26 May 2016
SRR1545899_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545899_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences691681
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115631.6717243931812498No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10930.1580208217371881No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA9300.13445504502798256No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGCG551.8189894E-1244.0000041
GCGTTCG302.5262489E-644.00000444
ACGGGCG600.044.0000045
CTATACG207.8543054E-444.01
CGCATCG207.8543054E-444.021
AGTGCGT207.8543054E-444.06
GTCGTAC207.8543054E-444.016
CGAATTC207.8543054E-444.024
TTGCGAG454.783942E-1044.01
CACCGTG254.4409942E-544.024
TAAGACG254.4409942E-544.01
TAATGCG502.7284841E-1144.01
TCGAAGA207.8543054E-444.011
TCAGTCG207.8543054E-444.015
AACACGT502.7284841E-1144.041
CGTTTTT56000.041.8392831
GACGGGC900.041.5555574
TCACGAC800.041.2525
CGACGGT800.041.2528
ACGGGGC800.041.255
Produced by FastQC (version 0.11.5)