FastQCFastQC Report
Thu 26 May 2016
SRR1545898_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545898_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1729996
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105240.6083251059540021No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA32530.1880351168442008No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCA302.5284608E-644.00000435
TACGCCC302.5284608E-644.00000440
TGCGCAA302.5284608E-644.00000441
CTAGACG254.4437766E-544.01
GATACGA207.857586E-444.011
ACACGAT207.857586E-444.024
ACGCCTA207.857586E-444.036
CGAGTTA207.857586E-444.017
CGACGAG207.857586E-444.02
CGCATCG950.044.021
TCCTCGA408.3164196E-944.014
TCGATTG254.4437766E-544.01
CGTTCGA207.857586E-444.014
TACGACA254.4437766E-544.011
TTAACGC351.4466605E-744.01
ATGCGTC207.857586E-444.011
CGAGAAT207.857586E-444.021
GACGCGT207.857586E-444.037
AATACGG700.044.02
AATCGCG207.857586E-444.01
Produced by FastQC (version 0.11.5)