##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545898_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1729996
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.747395947736294	31.0	31.0	33.0	30.0	34.0
2	32.17975475087804	33.0	31.0	34.0	30.0	34.0
3	32.25040346914097	34.0	31.0	34.0	30.0	34.0
4	35.93714262923151	37.0	35.0	37.0	35.0	37.0
5	35.92736399390519	37.0	35.0	37.0	35.0	37.0
6	35.96940917782469	37.0	35.0	37.0	35.0	37.0
7	36.12021472882018	37.0	35.0	37.0	35.0	37.0
8	36.061896674905604	37.0	35.0	37.0	35.0	37.0
9	37.86193667499809	39.0	38.0	39.0	35.0	39.0
10	37.65254254923133	39.0	37.0	39.0	35.0	39.0
11	37.386137308988	39.0	37.0	39.0	35.0	39.0
12	36.4886167366861	38.0	35.0	39.0	33.0	39.0
13	36.22402190525296	38.0	35.0	39.0	33.0	39.0
14	37.213537487947946	39.0	35.0	41.0	33.0	41.0
15	37.419131604928566	39.0	35.0	41.0	33.0	41.0
16	37.50777631855796	39.0	35.0	41.0	33.0	41.0
17	37.42305473538667	39.0	35.0	41.0	33.0	41.0
18	37.380697412017135	39.0	35.0	41.0	33.0	41.0
19	37.30420012531821	38.0	35.0	41.0	34.0	41.0
20	37.135345399642546	38.0	35.0	41.0	33.0	41.0
21	36.99603756309263	38.0	35.0	41.0	33.0	41.0
22	36.97234386669102	38.0	35.0	41.0	33.0	41.0
23	36.954509721409764	38.0	35.0	41.0	33.0	41.0
24	36.91259286148639	38.0	35.0	41.0	33.0	41.0
25	36.8276660755285	38.0	35.0	41.0	33.0	41.0
26	36.73111960952511	38.0	35.0	41.0	33.0	41.0
27	36.62990319052761	38.0	35.0	41.0	32.0	41.0
28	36.59921699240923	38.0	35.0	41.0	32.0	41.0
29	36.549420923516585	38.0	35.0	41.0	32.0	41.0
30	36.46100742429462	37.0	35.0	41.0	32.0	41.0
31	36.25326532546896	37.0	35.0	40.0	31.0	41.0
32	36.05975620752881	37.0	35.0	41.0	31.0	41.0
33	35.89374137281242	37.0	35.0	41.0	30.0	41.0
34	35.749243928887694	37.0	35.0	40.0	30.0	41.0
35	35.55411688813153	37.0	35.0	40.0	30.0	41.0
36	35.3891488766448	37.0	35.0	40.0	29.0	41.0
37	35.29868045937678	37.0	35.0	40.0	28.0	41.0
38	35.26113297371786	37.0	35.0	40.0	29.0	41.0
39	35.254044518022006	37.0	35.0	40.0	28.0	41.0
40	35.081116372523404	36.0	35.0	40.0	27.0	41.0
41	34.972918434493494	36.0	34.0	40.0	27.0	41.0
42	34.92571890339631	36.0	34.0	40.0	26.0	41.0
43	34.847384040194314	36.0	34.0	40.0	26.0	41.0
44	34.71893518828945	36.0	34.0	40.0	26.0	41.0
45	34.6260794822647	35.0	34.0	40.0	26.0	41.0
46	34.53430585966673	35.0	34.0	40.0	25.0	41.0
47	34.45671146060453	35.0	34.0	40.0	25.0	41.0
48	34.464005697123	35.0	34.0	40.0	25.0	41.0
49	34.46141089343559	36.0	34.0	40.0	25.0	41.0
50	34.305460243838716	35.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	6.0
12	2.0
13	15.0
14	24.0
15	52.0
16	106.0
17	239.0
18	563.0
19	1171.0
20	2145.0
21	3440.0
22	5291.0
23	8021.0
24	11493.0
25	16688.0
26	23299.0
27	28660.0
28	31239.0
29	31723.0
30	34978.0
31	40866.0
32	51719.0
33	70692.0
34	145003.0
35	331646.0
36	91045.0
37	118132.0
38	204394.0
39	477342.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.52343473626529	22.899648322886296	27.288213383152332	14.288703557696087
2	33.307475855435506	24.936473841558016	29.087003669372645	12.669046633633835
3	29.24850693296401	25.063930783654992	32.027877521104095	13.659684762276909
4	25.829539490264718	26.91746108083487	31.87458236897658	15.378417059923837
5	23.328551048672942	31.312326733703433	30.770417966284313	14.58870425133931
6	20.643515938765177	40.7225797053866	28.744286113956335	9.889618241891888
7	85.77898446007967	4.507178051278731	8.056203598158609	1.657633890482984
8	87.2269068830217	3.390990499399998	7.627185265168243	1.7549173524100632
9	83.26348731442154	4.5476405725793585	9.415050670637388	2.7738214423617165
10	54.07792850388093	21.446870397388203	14.578819835421585	9.89638126330928
11	45.65617492757209	21.151204973884333	19.59143258134701	13.601187517196573
12	39.99610403723477	21.313633095105423	24.83988402285323	13.850378844806578
13	19.603051105320475	40.74541212812053	26.562026733009787	13.089510033549209
14	13.806852732607474	41.865472521323746	30.894695710279095	13.432979035789677
15	13.094943572123865	21.0938059972393	51.913472632306664	13.897777798330168
16	16.69437385982395	16.494604611802572	49.275663065116916	17.53535846325656
17	17.136687021241666	16.9323512886735	29.79018448597569	36.14077720410914
18	20.538891419402123	21.622419936231065	38.04604172495197	19.792646919414842
19	27.95827273589072	21.93623569071836	29.666484777999486	20.439006795391435
20	30.30874059824416	21.07045334208865	27.859601987519046	20.761204072148143
21	21.510743377441337	26.56728686077887	30.61810547538838	21.303864286391413
22	23.292365993909815	23.309129038448646	27.885498001151447	25.5130069664901
23	19.476403413649514	30.93978251972837	25.42768885014763	24.15612521647449
24	18.25495550278729	23.80849435490024	39.81338685176151	18.123163290550963
25	15.40049803583361	25.045491434662274	38.54575386301472	21.0082566664894
26	16.71570338890957	33.92175473238088	29.118391025181563	20.244150853527984
27	17.745474556010535	34.691640905528104	29.418102700815492	18.14478183764587
28	15.077318097845312	28.6009331813484	38.97633289325525	17.345415827551047
29	15.981539841710617	22.671208488343325	39.82159496322535	21.52565670672071
30	17.53605210647886	29.246426003297117	34.718982009206954	18.498539881017066
31	28.850355723365833	26.564338877084108	25.303584516958423	19.281720882591635
32	30.664348356874815	26.14104310067769	26.65798071209413	16.536627830353364
33	30.22365369630912	26.289540553850994	25.54057928457638	17.946226465263504
34	20.829007697127626	28.05902441392928	28.20902476075089	22.902943128192206
35	19.651143702066364	27.42757786723206	32.600248786702394	20.32102964399918
36	31.971634616496225	24.91630038450956	26.27006074002483	16.842004258969386
37	21.820281665391132	33.730887239045636	27.731046776986766	16.71778431857646
38	19.78160643145996	35.433203313764885	23.62606618743627	21.159124067338883
39	19.59969849641271	34.199096414095756	26.743587846445887	19.45761724304565
40	23.690517203507984	26.45988776852663	24.98763002920238	24.861964998763003
41	17.011137598006005	24.456877356941867	27.496190742637555	31.035794302414573
42	21.51114800265434	26.00179422380167	23.940170959932853	28.546886813611128
43	21.372188143787614	27.34757768226054	26.608327418098078	24.67190675585377
44	19.466229979722495	29.847294444611432	29.812785694302185	20.873689881363887
45	19.269639929803304	36.66968016111019	23.060342336051644	21.000337573034848
46	22.63155521746871	31.60556440592926	26.866825125607225	18.8960552509948
47	21.89739167026976	27.736538119163278	26.98994679756485	23.376123413002112
48	23.576644107847645	23.84930369781202	30.251341621599124	22.322710572741208
49	20.760105803712843	23.56502558387418	33.215683735684934	22.459184876728038
50	19.88623095082301	32.82383311868929	27.533416262234134	19.75651966825357
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1777.0
1	3498.5
2	5220.0
3	12445.0
4	19670.0
5	14432.0
6	9194.0
7	9184.5
8	9175.0
9	9412.0
10	9649.0
11	9510.5
12	9372.0
13	8558.0
14	7744.0
15	7509.0
16	7274.0
17	6869.0
18	6464.0
19	6481.0
20	6498.0
21	6646.0
22	6794.0
23	7025.5
24	7257.0
25	8440.5
26	9624.0
27	10829.5
28	12035.0
29	14706.0
30	17377.0
31	21265.0
32	25153.0
33	29274.5
34	33396.0
35	37686.0
36	41976.0
37	46204.5
38	50433.0
39	64169.5
40	77906.0
41	107091.5
42	136277.0
43	160965.0
44	185653.0
45	194222.0
46	202791.0
47	192342.0
48	181893.0
49	165535.5
50	149178.0
51	138206.5
52	127235.0
53	113514.0
54	99793.0
55	90549.5
56	81306.0
57	69963.5
58	58621.0
59	52797.5
60	46974.0
61	39837.5
62	32701.0
63	27082.5
64	21464.0
65	16999.5
66	12535.0
67	9596.5
68	6658.0
69	5761.5
70	4865.0
71	4074.0
72	3283.0
73	2720.5
74	2158.0
75	1620.5
76	1083.0
77	880.5
78	678.0
79	558.0
80	438.0
81	307.0
82	176.0
83	149.0
84	122.0
85	95.5
86	69.0
87	48.5
88	28.0
89	21.5
90	15.0
91	13.0
92	11.0
93	6.5
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1729996.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.820909728677446
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.68664428283853	11.810285614971804
2	6.143337523971444	1.8209970175115515
3	2.1279230202449777	0.9461326497787645
4	1.1828188556037327	0.7012180593712194
5	0.7638214352904724	0.5660264270634465
6	0.5554842564107692	0.49396692119793156
7	0.4484259789631873	0.4652256667945047
8	0.3845841396143897	0.45599094530447626
9	0.33203139997079045	0.44289066654481324
>10	4.642515786028535	16.633307718114875
>50	1.8023440766811651	19.6971075433959
>100	1.9233676150612489	44.60318733686477
>500	0.005913202341856679	0.5587280810025999
>1k	3.9421348945711186E-4	0.1900598606610329
>5k	0.0	0.0
>10k+	3.9421348945711186E-4	0.6148754914222903
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10524	0.6083251059540021	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3253	0.1880351168442008	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02098270747446815	0.0
2	0.0	0.0	0.0	0.056763137024594276	0.0
3	0.0	0.0	0.0	0.08317938307371808	0.0
4	0.0	0.0	0.0	0.11890200902198618	0.0
5	0.0	0.0	0.0	0.19757271115077724	0.0
6	0.0	0.0	0.0	0.28601222199357684	0.0
7	0.0	0.0	0.0	0.347052825555666	0.0
8	0.0	0.0	0.0	0.496821957969845	0.0
9	0.0	0.0	5.780360185803898E-5	0.5737585520428949	0.0
10	0.0	0.0	5.780360185803898E-5	0.6883252909255282	0.0
11	0.0	0.0	5.780360185803898E-5	0.7958977939833387	0.0
12	0.0	0.0	5.780360185803898E-5	0.8922563982806897	0.0
13	0.0	0.0	5.780360185803898E-5	0.9325455087757428	0.0
14	0.0	0.0	5.780360185803898E-5	0.94618715881424	0.0
15	0.0	0.0	5.780360185803898E-5	0.969828831974178	0.0
16	0.0	0.0	5.780360185803898E-5	1.0207538052111103	0.0
17	0.0	0.0	5.780360185803898E-5	1.0808117475416128	0.0
18	0.0	0.0	5.780360185803898E-5	1.177343762644538	0.0
19	0.0	0.0	5.780360185803898E-5	1.2165346047042884	0.0
20	0.0	0.0	5.780360185803898E-5	1.2623728609777132	0.0
21	0.0	0.0	5.780360185803898E-5	1.3175174971502823	0.0
22	0.0	0.0	5.780360185803898E-5	1.3679222379704925	0.0
23	0.0	0.0	5.780360185803898E-5	1.4301767171716004	0.0
24	0.0	0.0	5.780360185803898E-5	1.4767664202691797	0.0
25	0.0	0.0	5.780360185803898E-5	1.5144543686806213	0.0
26	0.0	0.0	5.780360185803898E-5	1.5545122647682423	0.0
27	0.0	0.0	5.780360185803898E-5	1.5946279644577213	0.0
28	0.0	0.0	5.780360185803898E-5	1.6407552387404365	0.0
29	0.0	0.0	5.780360185803898E-5	1.6885588174770347	0.0
30	0.0	0.0	5.780360185803898E-5	1.7535878695673284	0.0
31	0.0	0.0	5.780360185803898E-5	1.8124319362588122	0.0
32	0.0	0.0	5.780360185803898E-5	1.87144941375587	0.0
33	0.0	0.0	5.780360185803898E-5	1.9312761416789404	0.0
34	0.0	0.0	5.780360185803898E-5	1.9873456354812382	0.0
35	0.0	0.0	5.780360185803898E-5	2.06746142765648	0.0
36	0.0	0.0	5.780360185803898E-5	2.137056964293559	0.0
37	0.0	0.0	5.780360185803898E-5	2.2069415189399284	0.0
38	0.0	0.0	5.780360185803898E-5	2.2702364629744807	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGCA	30	2.5284608E-6	44.000004	35
TACGCCC	30	2.5284608E-6	44.000004	40
TGCGCAA	30	2.5284608E-6	44.000004	41
CTAGACG	25	4.4437766E-5	44.0	1
GATACGA	20	7.857586E-4	44.0	11
ACACGAT	20	7.857586E-4	44.0	24
ACGCCTA	20	7.857586E-4	44.0	36
CGAGTTA	20	7.857586E-4	44.0	17
CGACGAG	20	7.857586E-4	44.0	2
CGCATCG	95	0.0	44.0	21
TCCTCGA	40	8.3164196E-9	44.0	14
TCGATTG	25	4.4437766E-5	44.0	1
CGTTCGA	20	7.857586E-4	44.0	14
TACGACA	25	4.4437766E-5	44.0	11
TTAACGC	35	1.4466605E-7	44.0	1
ATGCGTC	20	7.857586E-4	44.0	11
CGAGAAT	20	7.857586E-4	44.0	21
GACGCGT	20	7.857586E-4	44.0	37
AATACGG	70	0.0	44.0	2
AATCGCG	20	7.857586E-4	44.0	1
>>END_MODULE