FastQCFastQC Report
Thu 26 May 2016
SRR1545897_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545897_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2033708
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126090.6200005113811815No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA49310.24246351983667272No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATG850.044.01
CTATACG207.8579126E-444.01
CGGTTAG302.5286827E-644.037
GCACGAC254.4440516E-544.037
TCCTCGA254.4440516E-544.032
CACGACC207.8579126E-444.027
ATCGTCC502.7284841E-1144.022
GTAGCGC254.4440516E-544.034
CCGTAGA207.8579126E-444.043
AACGCGT254.4440516E-544.024
AACGCGC207.8579126E-444.027
AACGCAT254.4440516E-544.023
GTATCGC207.8579126E-444.022
CTCGATA207.8579126E-444.023
TTACGGC207.8579126E-444.036
ACGTAGC207.8579126E-444.027
ACGTACT351.4468242E-744.028
CACGTAC502.7284841E-1144.027
GCTCGAT207.8579126E-444.039
TATCGCT207.8579126E-444.023
Produced by FastQC (version 0.11.5)