##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545897_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2033708
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.772926103452413	31.0	31.0	33.0	30.0	34.0
2	32.19589931297905	33.0	31.0	34.0	30.0	34.0
3	32.22342932220358	34.0	31.0	34.0	30.0	34.0
4	35.903587929043894	37.0	35.0	37.0	35.0	37.0
5	35.893596327496375	37.0	35.0	37.0	35.0	37.0
6	35.94638463338887	37.0	35.0	37.0	35.0	37.0
7	36.141975642520954	37.0	35.0	37.0	35.0	37.0
8	36.10200923633088	37.0	35.0	37.0	35.0	37.0
9	37.892728946338416	39.0	39.0	39.0	35.0	39.0
10	37.665010414474445	39.0	37.0	39.0	35.0	39.0
11	37.382307096200634	39.0	37.0	39.0	35.0	39.0
12	36.34092308236974	38.0	35.0	39.0	33.0	39.0
13	36.00425872347456	37.0	35.0	39.0	33.0	39.0
14	36.94629858367081	38.0	35.0	41.0	33.0	41.0
15	37.191822031481415	39.0	35.0	41.0	33.0	41.0
16	37.317998945768025	38.0	35.0	41.0	33.0	41.0
17	37.27187383832881	38.0	35.0	41.0	33.0	41.0
18	37.223240012823865	38.0	35.0	41.0	33.0	41.0
19	37.126563892161506	38.0	35.0	41.0	33.0	41.0
20	36.93284975030831	38.0	35.0	41.0	33.0	41.0
21	36.80024959335362	38.0	35.0	41.0	33.0	41.0
22	36.75476764609275	38.0	35.0	41.0	33.0	41.0
23	36.76324526431523	37.0	35.0	41.0	33.0	41.0
24	36.7109201517622	37.0	35.0	41.0	33.0	41.0
25	36.611398489852036	37.0	35.0	41.0	33.0	41.0
26	36.50721145808543	37.0	35.0	41.0	33.0	41.0
27	36.420203392030714	37.0	35.0	41.0	32.0	41.0
28	36.44237717509102	37.0	35.0	41.0	32.0	41.0
29	36.42385534206484	36.0	35.0	40.0	32.0	41.0
30	36.33659060199399	36.0	35.0	40.0	32.0	41.0
31	36.10493541845732	36.0	35.0	40.0	31.0	41.0
32	35.88581448270843	36.0	35.0	40.0	31.0	41.0
33	35.71705869279169	36.0	35.0	40.0	30.0	41.0
34	35.554482256056424	36.0	35.0	40.0	30.0	41.0
35	35.3528117114158	36.0	35.0	40.0	29.0	41.0
36	35.17824486111084	36.0	35.0	40.0	28.0	41.0
37	35.08393781211462	36.0	34.0	40.0	28.0	41.0
38	35.04220861598617	36.0	34.0	40.0	28.0	41.0
39	35.02885665002055	36.0	35.0	40.0	28.0	41.0
40	34.85355616440511	36.0	34.0	40.0	27.0	41.0
41	34.79653027868307	36.0	34.0	40.0	27.0	41.0
42	34.75592612115407	36.0	34.0	40.0	27.0	41.0
43	34.669120149008606	35.0	34.0	40.0	26.0	41.0
44	34.54492631193859	35.0	34.0	40.0	26.0	41.0
45	34.42663106011286	35.0	34.0	40.0	26.0	41.0
46	34.417347033104065	35.0	34.0	40.0	26.0	41.0
47	34.38292026190584	35.0	34.0	40.0	26.0	41.0
48	34.34696082230094	35.0	34.0	40.0	26.0	41.0
49	34.32635166897116	36.0	34.0	40.0	25.0	41.0
50	34.12339677082452	35.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	8.0
13	8.0
14	24.0
15	49.0
16	128.0
17	308.0
18	689.0
19	1413.0
20	2480.0
21	4167.0
22	6298.0
23	9462.0
24	13723.0
25	20449.0
26	28636.0
27	34904.0
28	36569.0
29	37860.0
30	42252.0
31	49881.0
32	62600.0
33	86013.0
34	180876.0
35	422783.0
36	107679.0
37	135573.0
38	232618.0
39	516255.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.58087788414069	22.85303494897006	29.422070425056106	14.144016741833143
2	32.42068182846308	24.985838674972022	30.277355451225052	12.316124045339842
3	28.863435655462826	24.30093209054594	34.04112094754999	12.794511306441239
4	25.438607705727666	26.94929655584774	33.017375159069054	14.594720579355542
5	22.4085266911474	31.589146524476476	31.740987398387578	14.26133938598855
6	20.975036730936793	39.118054312615186	29.175574861287856	10.73133409516017
7	85.99051584593265	4.557979808310731	7.819313293747185	1.6321910520094332
8	88.19781404213387	3.1261616711937017	7.281969682963337	1.394054603709087
9	84.06024857059126	4.755894159830222	8.823292232709907	2.3605650368686164
10	55.56318802895991	21.012898606879652	13.727486935194236	9.696426428966204
11	50.54324416287884	18.218200449622067	20.133765515993446	11.104789871505643
12	43.09153526464959	21.61372232395211	23.33840453005053	11.956337881347764
13	19.384247886127213	44.04875232825952	24.650588973441614	11.916410812171659
14	13.778231683211159	44.69874731279023	29.82261956977108	11.70040143422753
15	12.549785908301486	21.495858795854666	53.125079903309626	12.829275392534228
16	14.613504003524596	16.57096298977041	50.281505506198535	18.534027500506465
17	15.42428903264382	16.710560218084407	30.17173556872471	37.693415180547056
18	21.233038371290274	21.156429536590306	37.652898056161455	19.957634035957962
19	30.416165939259717	22.106418423883863	27.741986558542326	19.73542907831409
20	32.13435753805364	21.366489191171986	26.448929738192504	20.050223532581864
21	21.93166373933721	27.248602060866162	30.632470344808592	20.187263854988032
22	22.74544821577139	23.55170948828445	26.09504412629542	27.60779816964874
23	18.248883320516025	30.283206832052585	26.678461214687655	24.78944863274374
24	18.2805987880266	22.0867007456331	43.08612642522919	16.546574041111114
25	15.400195111589273	23.91547852494065	41.20326025171755	19.481066111752522
26	15.019511158927438	35.547089355994075	29.39787816146664	20.035521323611846
27	15.868895633001395	35.25855235854902	31.111988545061532	17.760563463388056
28	14.105663153215703	27.728071089851642	41.87587401927907	16.290391737653586
29	14.452418931331342	23.539810041559555	40.76730779443263	21.240463232676472
30	17.56033806229803	31.411834934021993	32.89484036056307	18.132986643116908
31	30.32593666347381	26.031564020006805	26.114368434406515	17.52813088211287
32	30.88929187474308	25.68215299344842	27.993792619196068	15.43476251261243
33	29.306026233854617	26.322412067022405	25.28632429040944	19.085237408713542
34	19.772356700175248	27.43078160679901	28.33656552464759	24.460296168378154
35	20.458590908822703	25.353934783164544	32.67081606602324	21.516658241989507
36	32.149256432093495	23.88779510136165	27.091942402744152	16.871006063800703
37	19.986399227421046	33.79772317363161	29.301600819783367	16.91427677916397
38	20.326418541894903	34.523589423850424	24.278460821317516	20.871531212937157
39	20.64416327221017	32.597452534975524	27.70038766627264	19.057996526541668
40	24.920588403054914	25.967690543578527	25.35349224175742	23.75822881160914
41	17.409087243596424	24.56153980807471	28.093413607066502	29.935959341262365
42	22.5299797217693	24.774697252506257	24.76092929761795	27.934393728106492
43	22.57875761908789	25.285881749002314	27.228343498673357	24.907017133236433
44	18.867408693873458	30.39423555397333	29.806786421649516	20.93156933050369
45	16.6599138126024	39.769180236297444	23.910463055659907	19.66044289544025
46	21.928172579347674	33.362311600288734	26.843627502079944	17.86588831828365
47	21.569861553379344	28.161860011368393	27.012334120729232	23.255944314523028
48	22.53932226258637	24.875104980656022	32.585503916983164	20.00006883977444
49	21.25683726474007	24.35615142390156	34.222415410668596	20.164595900689775
50	19.7860754837961	35.239129707903004	26.621963428378116	18.352831379922783
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2658.0
1	4096.5
2	5535.0
3	14193.0
4	22851.0
5	16691.0
6	10531.0
7	11187.5
8	11844.0
9	11989.0
10	12134.0
11	11736.0
12	11338.0
13	10450.5
14	9563.0
15	8975.5
16	8388.0
17	7633.5
18	6879.0
19	6961.0
20	7043.0
21	7502.0
22	7961.0
23	9568.0
24	11175.0
25	10962.5
26	10750.0
27	13126.5
28	15503.0
29	19216.0
30	22929.0
31	28402.5
32	33876.0
33	37056.5
34	40237.0
35	45912.5
36	51588.0
37	59099.5
38	66611.0
39	85514.5
40	104418.0
41	144839.0
42	185260.0
43	213878.5
44	242497.0
45	236868.5
46	231240.0
47	208131.5
48	185023.0
49	175183.0
50	165343.0
51	151612.5
52	137882.0
53	126476.0
54	115070.0
55	99491.5
56	83913.0
57	74717.5
58	65522.0
59	57825.0
60	50128.0
61	43046.0
62	35964.0
63	29403.0
64	22842.0
65	18728.0
66	14614.0
67	11817.0
68	9020.0
69	7533.0
70	6046.0
71	5095.5
72	4145.0
73	3349.0
74	2553.0
75	1820.5
76	1088.0
77	916.0
78	744.0
79	656.5
80	569.0
81	392.0
82	215.0
83	146.5
84	78.0
85	69.0
86	60.0
87	40.5
88	21.0
89	28.0
90	35.0
91	23.0
92	11.0
93	10.5
94	10.0
95	6.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2033708.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.28685755819802
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.12096860867796	11.44676865937037
2	6.578568851709835	1.8797415224235348
3	2.2664423896748205	0.9714101875543831
4	1.2061659375238332	0.6892928376381351
5	0.766145518619026	0.5472905946680888
6	0.5695722867919476	0.4882438878296201
7	0.4346494139515732	0.4346841985416262
8	0.3376770977919598	0.3859475677455546
9	0.2965549032210152	0.38131538944534593
>10	3.860474478658364	13.114503778967606
>50	1.378411205442661	14.45023844618609
>100	2.171493465274436	53.13559652416959
>500	0.011483859672124357	1.0607262072718373
>1k	0.0010439872429203962	0.38735026392570354
>5k	0.0	0.0
>10k+	3.47995747640132E-4	0.6268899342625076
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12609	0.6200005113811815	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	4931	0.24246351983667272	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.022618783030798915	0.0
2	0.0	0.0	0.0	0.07282264710568086	0.0
3	0.0	0.0	0.0	0.10237457884809421	0.0
4	0.0	0.0	0.0	0.14456352632728003	0.0
5	0.0	0.0	0.0	0.2420209784295484	0.0
6	0.0	0.0	0.0	0.34351047446339394	0.0
7	0.0	0.0	0.0	0.4109734534161246	0.0
8	0.0	0.0	0.0	0.6011679159446686	0.0
9	0.0	0.0	0.0	0.6981336553723544	0.0
10	0.0	0.0	0.0	0.8665452464168898	0.0
11	0.0	0.0	0.0	1.0051098781142622	0.0
12	0.0	0.0	0.0	1.1371839025071446	0.0
13	0.0	0.0	0.0	1.1853225733487798	0.0
14	0.0	0.0	0.0	1.2006640087957563	0.0
15	0.0	0.0	0.0	1.225544670129635	0.0
16	0.0	0.0	0.0	1.2832225668581723	0.0
17	0.0	0.0	0.0	1.3521606838346507	0.0
18	0.0	0.0	0.0	1.4574855387302406	0.0
19	0.0	0.0	0.0	1.5038540439433783	0.0
20	0.0	0.0	0.0	1.5600568026481678	0.0
21	0.0	0.0	0.0	1.625307074565277	0.0
22	0.0	0.0	0.0	1.691737456901384	0.0
23	0.0	0.0	0.0	1.769526402020349	0.0
24	0.0	0.0	0.0	1.8268109286092202	0.0
25	0.0	0.0	0.0	1.871114240589111	0.0
26	0.0	0.0	0.0	1.921318104663993	0.0
27	0.0	0.0	0.0	1.9676866098771308	0.0
28	0.0	0.0	0.0	2.022119202953423	0.0
29	0.0	0.0	0.0	2.077633563913797	0.0
30	0.0	0.0	0.0	2.154734111288346	0.0
31	0.0	0.0	0.0	2.2234263719275336	0.0
32	0.0	0.0	0.0	2.2877423897629354	0.0
33	0.0	0.0	0.0	2.352500949005462	0.0
34	0.0	0.0	0.0	2.4215865797843152	0.0
35	0.0	0.0	0.0	2.5181097778048764	0.0
36	0.0	0.0	0.0	2.5916699939224315	0.0
37	0.0	0.0	0.0	2.6661152928542347	0.0
38	0.0	0.0	0.0	2.7341191557490063	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCATG	85	0.0	44.0	1
CTATACG	20	7.8579126E-4	44.0	1
CGGTTAG	30	2.5286827E-6	44.0	37
GCACGAC	25	4.4440516E-5	44.0	37
TCCTCGA	25	4.4440516E-5	44.0	32
CACGACC	20	7.8579126E-4	44.0	27
ATCGTCC	50	2.7284841E-11	44.0	22
GTAGCGC	25	4.4440516E-5	44.0	34
CCGTAGA	20	7.8579126E-4	44.0	43
AACGCGT	25	4.4440516E-5	44.0	24
AACGCGC	20	7.8579126E-4	44.0	27
AACGCAT	25	4.4440516E-5	44.0	23
GTATCGC	20	7.8579126E-4	44.0	22
CTCGATA	20	7.8579126E-4	44.0	23
TTACGGC	20	7.8579126E-4	44.0	36
ACGTAGC	20	7.8579126E-4	44.0	27
ACGTACT	35	1.4468242E-7	44.0	28
CACGTAC	50	2.7284841E-11	44.0	27
GCTCGAT	20	7.8579126E-4	44.0	39
TATCGCT	20	7.8579126E-4	44.0	23
>>END_MODULE