FastQCFastQC Report
Thu 26 May 2016
SRR1545896_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545896_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1979058
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116910.5907355923878936No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA29780.1504756303251345No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGTA207.857861E-444.00000421
CGCATTA207.857861E-444.00000440
ATAATCG207.857861E-444.00000410
AGGACGC207.857861E-444.00000426
CGTAGTC207.857861E-444.00000417
CCGTTCG207.857861E-444.00000442
CCGTTCA207.857861E-444.00000439
GGTACGT302.5286463E-644.018
CGCATGA302.5286463E-644.014
CCGCTAA302.5286463E-644.024
TACGACT254.4440087E-544.010
CTACCGG302.5286463E-644.02
CCGAATA254.4440087E-544.028
TCGAGGT502.7284841E-1144.042
AGTACGC254.4440087E-544.040
TCGCCGT454.802132E-1043.99999625
CGATTCG900.043.99999610
TGGGCGA7700.041.428576
CGTTTTT73750.041.2555921
CGATAAG800.041.25000410
Produced by FastQC (version 0.11.5)