##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545896_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1979058
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.745707301150347	31.0	31.0	33.0	30.0	34.0
2	32.165838494879885	33.0	31.0	34.0	30.0	34.0
3	32.22274334557148	34.0	31.0	34.0	30.0	34.0
4	35.9039340938972	37.0	35.0	37.0	35.0	37.0
5	35.89011236659057	37.0	35.0	37.0	35.0	37.0
6	35.945866164609626	37.0	35.0	37.0	35.0	37.0
7	36.14950244004976	37.0	35.0	37.0	35.0	37.0
8	36.10994169953584	37.0	35.0	37.0	35.0	37.0
9	37.93853894125387	39.0	39.0	39.0	35.0	39.0
10	37.66110391913729	39.0	37.0	39.0	35.0	39.0
11	37.34543808215828	39.0	37.0	39.0	35.0	39.0
12	36.478788898556786	38.0	35.0	39.0	33.0	39.0
13	36.229150939487376	38.0	35.0	39.0	33.0	39.0
14	37.22145333790116	39.0	35.0	41.0	33.0	41.0
15	37.44270809647822	39.0	35.0	41.0	33.0	41.0
16	37.539755277510814	39.0	35.0	41.0	33.0	41.0
17	37.46016438123592	39.0	35.0	41.0	33.0	41.0
18	37.413336546983466	39.0	35.0	41.0	33.0	41.0
19	37.34262108538507	38.0	35.0	41.0	33.0	41.0
20	37.17224457292308	38.0	35.0	41.0	33.0	41.0
21	37.02633020356149	38.0	35.0	41.0	33.0	41.0
22	37.01589038825542	38.0	35.0	41.0	33.0	41.0
23	37.0141506716832	38.0	35.0	41.0	33.0	41.0
24	36.975189711468786	38.0	35.0	41.0	33.0	41.0
25	36.88448696298946	38.0	35.0	41.0	33.0	41.0
26	36.784962340669146	38.0	35.0	41.0	33.0	41.0
27	36.688876222930304	38.0	35.0	41.0	32.0	41.0
28	36.70533556874028	38.0	35.0	41.0	32.0	41.0
29	36.67113293294082	38.0	35.0	41.0	32.0	41.0
30	36.57932763971546	38.0	35.0	41.0	32.0	41.0
31	36.37609357583254	38.0	35.0	41.0	31.0	41.0
32	36.19882337960787	38.0	35.0	41.0	31.0	41.0
33	36.03096119466938	38.0	35.0	41.0	30.0	41.0
34	35.90720989480854	38.0	35.0	41.0	30.0	41.0
35	35.70684790440705	38.0	35.0	41.0	30.0	41.0
36	35.54779546632792	37.0	35.0	40.0	29.0	41.0
37	35.47624829590644	37.0	35.0	40.0	29.0	41.0
38	35.41768760693218	37.0	35.0	40.0	29.0	41.0
39	35.39623699760189	37.0	35.0	40.0	29.0	41.0
40	35.24206314317215	37.0	35.0	40.0	27.0	41.0
41	35.17422076563698	37.0	34.0	40.0	27.0	41.0
42	35.143388925438266	37.0	35.0	40.0	27.0	41.0
43	35.066797435951855	37.0	34.0	40.0	27.0	41.0
44	34.94414160676443	36.0	34.0	40.0	27.0	41.0
45	34.84610809789304	36.0	34.0	40.0	27.0	41.0
46	34.814733069975716	36.0	34.0	40.0	27.0	41.0
47	34.755692859936396	36.0	34.0	40.0	26.0	41.0
48	34.70551090468293	36.0	34.0	40.0	26.0	41.0
49	34.66602494722237	36.0	34.0	40.0	26.0	41.0
50	34.466739226440055	35.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	2.0
12	1.0
13	9.0
14	14.0
15	41.0
16	92.0
17	260.0
18	525.0
19	1256.0
20	2240.0
21	3803.0
22	5860.0
23	8560.0
24	12627.0
25	18800.0
26	25801.0
27	31504.0
28	33398.0
29	35469.0
30	39632.0
31	47117.0
32	58720.0
33	80280.0
34	161433.0
35	360020.0
36	106277.0
37	138323.0
38	242352.0
39	564639.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.65490046274541	23.065114817251438	29.176608265144328	14.103376454858827
2	31.853639458772808	24.38038703261855	30.753621167242194	13.012352341366448
3	28.97474455018499	25.043682398393578	32.570849363687174	13.410723687734267
4	25.92339385707746	26.941605551732188	32.805203283582394	14.329797307607963
5	22.217135627151908	31.861623055008998	31.59270723748369	14.328534080355402
6	20.794640682587374	40.72765932074755	28.56616632761647	9.911533669048609
7	87.721178459651	3.8017076811291033	7.078670761544129	1.398443097675763
8	89.1777300109446	2.5827944405873904	6.757305748492465	1.4821697999755439
9	85.46990537922588	4.417657289478125	7.956714760254627	2.155722571041374
10	52.82391925855634	25.599401331340466	12.824636771635797	8.752042638467392
11	44.69399077743048	19.70558720360899	24.03022043820848	11.570201580752054
12	39.65391615607021	22.20647398914029	25.055152501846838	13.084457352942664
13	19.671277951429417	40.870404000287	25.797273248181714	13.661044800101866
14	14.711392996061761	41.261145454049355	30.84639257667032	13.18106897321857
15	13.83264159008983	22.296921060423696	49.94300318636442	13.92743416312205
16	16.28284769824836	18.80248077620767	46.73410279031741	18.180568735226558
17	16.91804888992642	18.767767291307276	30.591574375283592	33.72260944348271
18	21.65712172154631	22.088438034660935	36.626465722581145	19.62797452121161
19	28.281637021249505	23.364095443387715	28.5358994026451	19.818368132717687
20	30.746799740078362	22.993818271116865	26.972680942145207	19.286701046659573
21	21.5276661927038	27.931419897749333	30.500520954918958	20.040392954627908
22	22.462605946869672	23.83851307035974	28.185682279144924	25.513198703625665
23	18.31108537496122	29.450678049860084	28.151474085145562	24.086762490033138
24	18.52548030426597	23.146264535955996	41.328652318426236	16.999602841351795
25	16.22959003728036	24.977135586728636	39.066970245440004	19.726304130551
26	16.41826565972296	34.645523274204194	29.36639552756918	19.569815538503672
27	16.80395420447506	33.72847081793459	31.05800840601943	18.409566571570917
28	14.584059689003556	28.066837859223938	39.91697059914363	17.432131852628878
29	15.452755806045099	24.64303724297115	39.30834770885947	20.595859242124284
30	19.028547925326087	29.956120538155023	32.81707761975647	18.198253916762418
31	29.174991334261048	25.825670596819293	26.965657398620962	18.0336806702987
32	29.71034704389664	25.966444641844756	27.9676997844429	16.3555085298157
33	26.98930501278891	27.002038343494732	26.731505595086148	19.277151048630206
34	18.877465945919724	28.28345606849319	29.21415137909046	23.624926606496626
35	19.967580535790262	26.210752792490165	33.13657305647435	20.685093615245233
36	29.218749526289777	26.0165694992264	27.041198388324144	17.723482586159676
37	20.165300865361196	32.466052030814666	29.755520050448244	17.613127053375898
38	20.100623630030046	33.10281962428589	25.892217408484235	20.904339337199822
39	20.4300732974981	31.119704425034538	28.507097821286692	19.943124456180666
40	23.873984491611665	25.91071105546174	26.567083935892732	23.64822051703386
41	17.749151363931727	25.230084211781566	28.500630097753575	28.52013432653313
42	21.738473556611275	25.56276774101618	25.930922691502722	26.767836010869818
43	21.59901326792848	26.027736428139043	27.571703305309903	24.801546998622577
44	19.22328703858098	30.388952723972718	29.594736485742207	20.793023751704094
45	17.47589004465761	37.70642396534109	24.355728836648545	20.461957153352756
46	21.377544266009384	32.19920790598355	26.714932053532543	19.70831577447452
47	20.432549222913124	28.48284385803751	28.34783012928373	22.736776789765635
48	22.58791809032378	25.33291091014008	31.983549749426242	20.0956212501099
49	21.004538522873002	24.49483542170063	33.57723725125793	20.923388804168447
50	19.560316069564408	32.99549583690827	27.692164656114173	19.75202343741315
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1999.0
1	3455.5
2	4912.0
3	13536.0
4	22160.0
5	16029.0
6	9898.0
7	9935.0
8	9972.0
9	10067.0
10	10162.0
11	9903.5
12	9645.0
13	9399.5
14	9154.0
15	8331.5
16	7509.0
17	7221.0
18	6933.0
19	6630.5
20	6328.0
21	6775.0
22	7222.0
23	8125.0
24	9028.0
25	11824.0
26	14620.0
27	16684.5
28	18749.0
29	23035.0
30	27321.0
31	32507.0
32	37693.0
33	44999.5
34	52306.0
35	54972.0
36	57638.0
37	65346.0
38	73054.0
39	92974.5
40	112895.0
41	146327.5
42	179760.0
43	197945.5
44	216131.0
45	211528.0
46	206925.0
47	190577.5
48	174230.0
49	165636.5
50	157043.0
51	148943.0
52	140843.0
53	125641.0
54	110439.0
55	97358.0
56	84277.0
57	74451.0
58	64625.0
59	55983.0
60	47341.0
61	39704.0
62	32067.0
63	26810.0
64	21553.0
65	17100.5
66	12648.0
67	10047.5
68	7447.0
69	6250.0
70	5053.0
71	4511.5
72	3970.0
73	3175.5
74	2381.0
75	1929.5
76	1478.0
77	1136.5
78	795.0
79	562.0
80	329.0
81	284.5
82	240.0
83	193.5
84	147.0
85	109.5
86	72.0
87	53.0
88	34.0
89	25.5
90	17.0
91	13.5
92	10.0
93	7.0
94	4.0
95	2.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1979058.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.33575489382565
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.0398150294001	11.330954151147967
2	6.917750392412311	1.983423480845782
3	2.229586385691437	0.9588841181964918
4	1.1753509763396708	0.6739817404409676
5	0.7872385060857536	0.564282913311342
6	0.5958457173759533	0.5125138895302427
7	0.46833873657775704	0.46997925344038916
8	0.36037519994263006	0.41330004289527644
9	0.3077595752232904	0.397076925296609
>10	4.431953728364271	15.081268178961771
>50	1.5861254371127553	16.60407376046383
>100	2.092373546946947	49.579215164774226
>500	0.006060717379127265	0.5525883381455576
>1k	0.0010695383610224585	0.2809450698100379
>5k	0.0	0.0
>10k+	3.565127870074862E-4	0.5975129727395221
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11691	0.5907355923878936	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2978	0.1504756303251345	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.022687561456005836	0.0
2	0.0	0.0	0.0	0.08256453322742437	0.0
3	0.0	0.0	0.0	0.11869283265068532	0.0
4	0.0	0.0	0.0	0.17649811172790286	0.0
5	0.0	0.0	0.0	0.30029438247893697	0.0
6	0.0	0.0	0.0	0.42191790235556514	0.0
7	0.0	0.0	0.0	0.5042803192225797	0.0
8	0.0	0.0	0.0	0.7119548795437021	0.0
9	0.0	0.0	0.0	0.7880011601479088	0.0
10	0.0	0.0	0.0	0.913667007232734	0.0
11	0.0	0.0	0.0	1.0368569289025384	0.0
12	0.0	0.0	0.0	1.143675425379145	0.0
13	0.0	0.0	0.0	1.1867767392365458	0.0
14	0.0	0.0	0.0	1.2004701226543133	0.0
15	0.0	0.0	0.0	1.2257346677055447	0.0
16	0.0	0.0	0.0	1.280659788646922	0.0
17	0.0	0.0	0.0	1.3494804093664763	0.0
18	0.0	0.0	0.0	1.458370598537284	0.0
19	0.0	0.0	0.0	1.5060700595940089	0.0
20	0.0	0.0	0.0	1.55902454602139	0.0
21	0.0	0.0	0.0	1.6339591866433425	0.0
22	0.0	0.0	0.0	1.7080853618236556	0.0
23	0.0	0.0	0.0	1.7997956603596257	0.0
24	0.0	0.0	0.0	1.864675012051188	0.0
25	0.0	0.0	0.0	1.9196001329925652	0.0
26	0.0	0.0	0.0	1.977001179348963	0.0
27	0.0	0.0	0.0	2.0304609566773686	0.0
28	0.0	0.0	0.0	2.0961487738105706	0.0
29	0.0	0.0	0.0	2.1612807709526454	0.0
30	0.0	0.0	0.0	2.2512730804251317	0.0
31	0.0	0.0	0.0	2.3395979299242367	0.0
32	5.052909010246289E-5	0.0	0.0	2.4144315123659843	0.0
33	5.052909010246289E-5	0.0	0.0	2.493863242007056	0.0
34	0.0010105818020492577	0.0	0.0	2.576175129783968	0.0
35	0.0010611108921517206	0.0	0.0	2.6778901881602257	0.0
36	0.0010611108921517206	0.0	0.0	2.7664676831098434	0.0
37	0.0010611108921517206	0.0	0.0	2.8565610507625343	0.0
38	0.0010611108921517206	0.0	0.0	2.943066853017951	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGGTA	20	7.857861E-4	44.000004	21
CGCATTA	20	7.857861E-4	44.000004	40
ATAATCG	20	7.857861E-4	44.000004	10
AGGACGC	20	7.857861E-4	44.000004	26
CGTAGTC	20	7.857861E-4	44.000004	17
CCGTTCG	20	7.857861E-4	44.000004	42
CCGTTCA	20	7.857861E-4	44.000004	39
GGTACGT	30	2.5286463E-6	44.0	18
CGCATGA	30	2.5286463E-6	44.0	14
CCGCTAA	30	2.5286463E-6	44.0	24
TACGACT	25	4.4440087E-5	44.0	10
CTACCGG	30	2.5286463E-6	44.0	2
CCGAATA	25	4.4440087E-5	44.0	28
TCGAGGT	50	2.7284841E-11	44.0	42
AGTACGC	25	4.4440087E-5	44.0	40
TCGCCGT	45	4.802132E-10	43.999996	25
CGATTCG	90	0.0	43.999996	10
TGGGCGA	770	0.0	41.42857	6
CGTTTTT	7375	0.0	41.255592	1
CGATAAG	80	0.0	41.250004	10
>>END_MODULE