FastQCFastQC Report
Thu 26 May 2016
SRR1545895_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545895_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1969910
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136200.6914021452756725No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA67270.34148768217837366No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25080.1273154611124366No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTGAT207.857852E-444.020
AACGTAC207.857852E-444.042
TAATACG600.044.01
GTACCGA254.4440014E-544.09
ACACGTA408.3182385E-944.019
TACCCGT207.857852E-444.013
CTATCGA254.4440014E-544.019
CTATCCG254.4440014E-544.01
GCGAACC302.5286408E-644.09
TCACACG254.4440014E-544.044
GACCGTT254.4440014E-544.033
CTATACG302.5286408E-644.01
CGACCGT207.857852E-444.017
GTTAGCG408.3182385E-944.01
CGTCTAT207.857852E-444.030
ACTTACG502.7284841E-1144.01
CCGTCTA254.4440014E-544.038
AATATCG207.857852E-444.041
ATATCGT207.857852E-444.042
CCACTAG302.5286408E-644.031
Produced by FastQC (version 0.11.5)