##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545895_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1969910
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.751723682807842	31.0	31.0	33.0	30.0	34.0
2	32.170462609966954	33.0	31.0	34.0	30.0	34.0
3	32.20512053850176	34.0	31.0	34.0	30.0	34.0
4	35.86984379997056	37.0	35.0	37.0	35.0	37.0
5	35.86648628617551	37.0	35.0	37.0	35.0	37.0
6	35.913340203359546	37.0	35.0	37.0	35.0	37.0
7	36.130357224441724	37.0	35.0	37.0	35.0	37.0
8	36.095927225101654	37.0	35.0	37.0	35.0	37.0
9	37.912231015630155	39.0	39.0	39.0	35.0	39.0
10	37.67569076759852	39.0	38.0	39.0	35.0	39.0
11	37.33390205643913	39.0	37.0	39.0	35.0	39.0
12	36.03537674309994	37.0	35.0	39.0	33.0	39.0
13	35.62926326583448	37.0	35.0	39.0	32.0	39.0
14	36.46690660994665	38.0	35.0	41.0	32.0	41.0
15	36.783287053723264	38.0	35.0	41.0	33.0	41.0
16	36.94917229721155	37.0	35.0	41.0	33.0	41.0
17	36.905038808879596	37.0	35.0	41.0	33.0	41.0
18	36.868524957993	37.0	35.0	40.0	33.0	41.0
19	36.745678228954624	37.0	35.0	40.0	33.0	41.0
20	36.521742110045636	36.0	35.0	40.0	33.0	41.0
21	36.36248407287643	35.0	35.0	40.0	32.0	41.0
22	36.327827667253835	35.0	35.0	40.0	32.0	41.0
23	36.35366590351843	35.0	35.0	40.0	32.0	41.0
24	36.308353681132644	35.0	35.0	40.0	33.0	41.0
25	36.206516541364834	35.0	35.0	40.0	32.0	41.0
26	36.079798569477795	35.0	35.0	40.0	32.0	41.0
27	35.98099913194004	35.0	35.0	40.0	32.0	41.0
28	36.033436552938966	36.0	35.0	40.0	32.0	41.0
29	36.033774131813125	36.0	35.0	40.0	32.0	41.0
30	35.95032463412034	36.0	35.0	40.0	32.0	41.0
31	35.69110365448168	35.0	35.0	40.0	31.0	41.0
32	35.458896599337024	35.0	35.0	40.0	31.0	41.0
33	35.274956723911245	35.0	35.0	40.0	30.0	41.0
34	35.132548187480644	35.0	35.0	40.0	30.0	41.0
35	34.92052276499942	35.0	34.0	40.0	29.0	41.0
36	34.727512424425484	35.0	34.0	40.0	27.0	41.0
37	34.60872831753735	35.0	34.0	40.0	27.0	41.0
38	34.575563350609926	35.0	34.0	40.0	27.0	41.0
39	34.58773751085075	35.0	34.0	40.0	27.0	41.0
40	34.36575122721342	35.0	34.0	40.0	25.0	41.0
41	34.313287916706855	35.0	34.0	40.0	25.0	41.0
42	34.29421394886061	35.0	34.0	40.0	25.0	41.0
43	34.21712413257458	35.0	34.0	40.0	25.0	41.0
44	34.09804864181612	35.0	34.0	40.0	24.0	41.0
45	33.978686335923975	35.0	34.0	40.0	24.0	41.0
46	33.97807717103827	35.0	34.0	39.0	24.0	41.0
47	33.92933941144519	35.0	34.0	39.0	24.0	41.0
48	33.91269905731734	35.0	34.0	39.0	24.0	41.0
49	33.89866998999954	35.0	34.0	39.0	24.0	41.0
50	33.642879116304805	35.0	34.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	0.0
13	11.0
14	18.0
15	46.0
16	86.0
17	313.0
18	724.0
19	1454.0
20	2752.0
21	4307.0
22	6656.0
23	10031.0
24	14557.0
25	21353.0
26	29920.0
27	36560.0
28	38048.0
29	39179.0
30	43415.0
31	52051.0
32	65248.0
33	90476.0
34	198565.0
35	482447.0
36	98856.0
37	113904.0
38	195087.0
39	423843.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.02212283809921	23.985207446025452	28.627094638841367	15.365575077033977
2	30.70079343726363	25.41811554842607	31.50473879517338	12.376352219136914
3	28.086765385220648	24.77412673675447	34.0072389093918	13.131868968633084
4	25.126020985730314	27.114081353970487	33.459904259585464	14.299993400713737
5	22.86317649029651	31.798508561304832	31.685863821189802	13.652451127208856
6	20.430933392896122	39.91649364691788	29.34707677000472	10.305496190181278
7	85.10180668152351	5.006776959353473	8.231137463132834	1.6602788959901722
8	87.57811270565661	3.0299861414988505	7.795127696189167	1.5967734566553802
9	84.6171144874639	4.036631115127087	9.058637196623195	2.2876172007858226
10	62.43209080617896	15.280444284256642	13.579858978328959	8.707605931235438
11	56.65167444197958	16.288155296434862	17.366834017797768	9.693336243787787
12	48.10072541385139	20.755009112091415	21.098324288926907	10.045941185130285
13	18.651613525491012	49.247275256229976	21.512353356244702	10.588757862034306
14	11.882522551791705	49.40743485743003	28.445868085344	10.264174505434259
15	11.021823332030397	20.13508231340518	58.34058408759791	10.50251026696651
16	11.974404922052278	13.837738779944262	55.264504469747344	18.923351828256113
17	13.260910396921687	13.873171870796128	30.477940616576394	42.387977115705795
18	20.911006086572485	20.11934555385779	39.07315562639918	19.89649273317055
19	32.429806437857565	20.571802772715504	27.16281454482692	19.835576244600006
20	33.335888441603934	20.406668324948857	25.81559563634887	20.441847597098345
21	20.151174419135902	28.24565589290881	30.31295846003117	21.290211227924118
22	21.817900310166454	24.327355056830008	25.120436974278014	28.734307658725527
23	16.465219223213243	31.247874268367593	25.573401830540483	26.713504677878685
24	17.389982283454575	20.98415663659761	45.85981085430299	15.766050225644825
25	13.722251270362607	23.097704971293105	43.42741546568117	19.752628292663115
26	13.236696092714894	36.90970653481631	29.69054423806164	20.163053134407154
27	15.13485387657304	37.25413851394226	31.309856795488116	16.30115081399658
28	11.965927377392877	28.822027402267107	44.48837764161814	14.723667578721871
29	12.378687351198787	23.425587971024058	42.679970150920596	21.515754526856558
30	16.90193968252357	32.04572797742029	33.96500347731622	17.087328862739923
31	33.07557198044581	25.704879918371905	25.203587981176806	16.01596012000548
32	33.41431842063851	25.138965739551555	27.36053931397881	14.086176525831128
33	30.451746526491057	26.208913097552678	24.736104695138355	18.603235680817907
34	18.335304658588463	28.31210562919118	28.796391713327008	24.55619799889335
35	19.910351234320352	24.730419156205105	33.485489184785095	21.87374042468945
36	35.0908924773213	22.461533775654726	26.075353696361763	16.37222005066221
37	19.837505266738074	34.25613352894295	29.551705407861274	16.354655796457706
38	19.609626835743764	35.63167860460631	23.45213740729272	21.306557152357215
39	20.39666786807519	33.18745526445371	27.39500789376164	19.020868973709458
40	25.121401485347043	24.817326679899082	24.366493900736582	25.69477793401729
41	16.33896980065079	22.769568152859776	27.984273393200702	32.90718865328873
42	22.28675421719774	23.921194369285907	24.05815494108868	29.733896472427674
43	21.70474793264667	25.357199059855528	27.06961231731399	25.868440690183814
44	18.015340802371682	31.266301506160183	30.00401033549756	20.714347355970578
45	14.706204851998313	42.68332055779198	21.575960323060446	21.03451426714926
46	21.708047575777574	35.04627114944338	25.376895391160005	17.868785883619047
47	21.363107959246868	27.993309339005336	26.986105964231864	23.65747673751593
48	23.480666629439924	23.457416836302166	32.758958531100404	20.302958003157503
49	21.432552756217287	23.016838332715707	35.224604169733645	20.32600474133336
50	19.80202141214573	36.41953185678533	25.910574594778442	17.86787213629049
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3656.0
1	4673.0
2	5690.0
3	14473.5
4	23257.0
5	16963.0
6	10669.0
7	11373.0
8	12077.0
9	12650.5
10	13224.0
11	12530.5
12	11837.0
13	11208.0
14	10579.0
15	9947.0
16	9315.0
17	8560.0
18	7805.0
19	7316.5
20	6828.0
21	7009.0
22	7190.0
23	7546.0
24	7902.0
25	9396.0
26	10890.0
27	12009.5
28	13129.0
29	16266.0
30	19403.0
31	22011.5
32	24620.0
33	26110.5
34	27601.0
35	34063.0
36	40525.0
37	46316.0
38	52107.0
39	76596.5
40	101086.0
41	147051.5
42	193017.0
43	227362.0
44	261707.0
45	250771.0
46	239835.0
47	214426.0
48	189017.0
49	173256.0
50	157495.0
51	143892.5
52	130290.0
53	118166.0
54	106042.0
55	92106.5
56	78171.0
57	69734.0
58	61297.0
59	52357.5
60	43418.0
61	38308.5
62	33199.0
63	27682.0
64	22165.0
65	17558.0
66	12951.0
67	10713.5
68	8476.0
69	6689.0
70	4902.0
71	4164.0
72	3426.0
73	2843.5
74	2261.0
75	1740.5
76	1220.0
77	942.5
78	665.0
79	550.0
80	435.0
81	321.0
82	207.0
83	179.0
84	151.0
85	112.5
86	74.0
87	66.0
88	58.0
89	39.5
90	21.0
91	17.0
92	13.0
93	8.0
94	3.0
95	2.5
96	2.0
97	1.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1969910.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.151878995774162
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.8162060123464	11.437011683842826
2	7.204313079495722	2.0390913389739316
3	2.3280100514473414	0.9883714964708628
4	1.19925052471467	0.6788659324552273
5	0.6950414089966008	0.49180709585861376
6	0.5215585257168136	0.4428619887095228
7	0.39417962496171904	0.3904867648540522
8	0.30634774632019235	0.3468316989241185
9	0.28621504594150954	0.36454326272407667
>10	3.080475527407266	10.211039599940158
>50	0.9939304633452914	10.165814038774716
>100	2.140048586709557	58.3146038149132
>500	0.03261164456550289	2.83868265055281
>1k	0.0010870548188500965	0.24660340817798046
>5k	3.623516062833655E-4	0.34495760590835406
>10k+	3.623516062833655E-4	0.6984276189195454
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13620	0.6914021452756725	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	6727	0.34148768217837366	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2508	0.1273154611124366	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04436750917554609	0.0
2	0.0	0.0	0.0	0.14371214928600798	0.0
3	0.0	0.0	0.0	0.19924768136615378	0.0
4	0.0	0.0	0.0	0.2730073962769873	0.0
5	0.0	0.0	0.0	0.4381418440436365	0.0
6	0.0	0.0	0.0	0.5825646856963008	0.0
7	0.0	0.0	0.0	0.680589468554401	0.0
8	0.0	0.0	0.0	0.8879593483966273	0.0
9	0.0	0.0	0.0	0.9850703839261692	0.0
10	0.0	0.0	0.0	1.1492910843642603	0.0
11	0.0	0.0	0.0	1.3080293008310024	0.0
12	0.0	0.0	0.0	1.446309729886137	0.0
13	0.0	0.0	0.0	1.4997131848663137	0.0
14	0.0	0.0	0.0	1.5203232634993478	0.0
15	0.0	0.0	0.0	1.555096425725033	0.0
16	0.0	0.0	0.0	1.637282921554792	0.0
17	0.0	0.0	0.0	1.73677985288668	0.0
18	0.0	0.0	0.0	1.9018127731723784	0.0
19	0.0	0.0	0.0	1.9598357285358214	0.0
20	0.0	0.0	0.0	2.033240097263327	0.0
21	0.0	0.0	0.0	2.1174571427121034	0.0
22	0.0	0.0	0.0	2.195684066784777	0.0
23	0.0	0.0	0.0	2.2978714763618644	0.0
24	0.0	0.0	0.0	2.36513343249184	0.0
25	0.0	0.0	0.0	2.416912447776802	0.0
26	0.0	0.0	0.0	2.4748846393997694	0.0
27	0.0	0.0	0.0	2.52534379743237	0.0
28	0.0	0.0	0.0	2.595143940586118	0.0
29	0.0	0.0	0.0	2.658497088699484	0.0
30	0.0	0.0	0.0	2.7450492662101316	0.0
31	0.0	0.0	0.0	2.8304846414303193	0.0
32	0.0	0.0	0.0	2.89891416359123	0.0
33	0.0	0.0	0.0	2.9659730647593037	0.0
34	0.0	0.0	0.0	3.037549938829693	0.0
35	0.0	0.0	0.0	3.1417171342853227	0.0
36	0.0	0.0	0.0	3.217710453777076	0.0
37	0.0	0.0	0.0	3.2945667568569124	0.0
38	0.0	0.0	0.0	3.370915422531994	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCTGAT	20	7.857852E-4	44.0	20
AACGTAC	20	7.857852E-4	44.0	42
TAATACG	60	0.0	44.0	1
GTACCGA	25	4.4440014E-5	44.0	9
ACACGTA	40	8.3182385E-9	44.0	19
TACCCGT	20	7.857852E-4	44.0	13
CTATCGA	25	4.4440014E-5	44.0	19
CTATCCG	25	4.4440014E-5	44.0	1
GCGAACC	30	2.5286408E-6	44.0	9
TCACACG	25	4.4440014E-5	44.0	44
GACCGTT	25	4.4440014E-5	44.0	33
CTATACG	30	2.5286408E-6	44.0	1
CGACCGT	20	7.857852E-4	44.0	17
GTTAGCG	40	8.3182385E-9	44.0	1
CGTCTAT	20	7.857852E-4	44.0	30
ACTTACG	50	2.7284841E-11	44.0	1
CCGTCTA	25	4.4440014E-5	44.0	38
AATATCG	20	7.857852E-4	44.0	41
ATATCGT	20	7.857852E-4	44.0	42
CCACTAG	30	2.5286408E-6	44.0	31
>>END_MODULE