FastQCFastQC Report
Thu 26 May 2016
SRR1545894_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545894_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1605826
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94110.5860535325745131No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA46700.2908160659996787No Hit
TTCGCCGGGGGAGGAGCTACTCTAGTATTAATAAATATTAGCCCACCAAC26270.16359182128076144No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19360.12056100723241497No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTA408.3164196E-944.033
AACGTAG454.802132E-1044.01
TCGTTAG1450.044.01
GGTACAA351.4465695E-744.08
AATCATC207.8574184E-444.018
TCACCGT408.3164196E-944.026
CGTATCG254.443633E-544.012
CGAGTAC408.3164196E-944.041
CGACGGT1650.044.028
CGTGCCT254.443633E-544.016
CGTGCCG207.8574184E-444.041
TTAGCCG254.443633E-544.03
TAGACGT254.443633E-544.039
TAGACCG254.443633E-544.01
CGGCCCA207.8574184E-444.020
CACATCG302.528348E-644.017
CTATACG700.044.01
CGTTGCG207.8574184E-444.036
GGCGTAA254.443633E-544.01
CGCATAA408.3164196E-944.018
Produced by FastQC (version 0.11.5)