##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545894_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1605826
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.774574580309448	31.0	31.0	33.0	30.0	34.0
2	32.19297047127148	33.0	31.0	34.0	30.0	34.0
3	32.23341881374445	34.0	31.0	34.0	30.0	34.0
4	35.90223785142351	37.0	35.0	37.0	35.0	37.0
5	35.874647689102055	37.0	35.0	37.0	35.0	37.0
6	35.92542342694663	37.0	35.0	37.0	35.0	37.0
7	36.14341902547349	37.0	35.0	37.0	35.0	37.0
8	36.11263424555338	37.0	35.0	37.0	35.0	37.0
9	37.983092813293595	39.0	39.0	39.0	35.0	39.0
10	37.717379093376245	39.0	38.0	39.0	35.0	39.0
11	37.32274356001211	39.0	37.0	39.0	35.0	39.0
12	35.86330461706312	35.0	35.0	39.0	33.0	39.0
13	35.405383895889095	35.0	35.0	39.0	32.0	39.0
14	36.18187960588507	36.0	35.0	41.0	32.0	41.0
15	36.52171468141629	36.0	35.0	41.0	33.0	41.0
16	36.732394418822466	36.0	35.0	41.0	33.0	41.0
17	36.68861694853614	36.0	35.0	41.0	33.0	41.0
18	36.67685290934385	36.0	35.0	40.0	33.0	41.0
19	36.56169286087036	36.0	35.0	40.0	33.0	41.0
20	36.32527932665183	35.0	35.0	40.0	33.0	41.0
21	36.1428828528122	35.0	35.0	40.0	32.0	41.0
22	36.10121457741997	35.0	35.0	40.0	32.0	41.0
23	36.16424693584486	35.0	35.0	40.0	32.0	41.0
24	36.11593659587029	35.0	35.0	40.0	32.0	41.0
25	36.00850092102133	35.0	35.0	40.0	32.0	41.0
26	35.86390244023948	35.0	35.0	40.0	32.0	41.0
27	35.782311408583496	35.0	35.0	40.0	32.0	41.0
28	35.83019579954491	36.0	35.0	40.0	32.0	41.0
29	35.86139158289877	36.0	35.0	40.0	32.0	41.0
30	35.820694770168124	36.0	35.0	40.0	32.0	41.0
31	35.526276819530885	35.0	35.0	40.0	31.0	41.0
32	35.28276413509309	35.0	35.0	40.0	31.0	41.0
33	35.104797157350795	35.0	35.0	40.0	30.0	41.0
34	35.00286145572434	35.0	35.0	40.0	30.0	41.0
35	34.808646142234586	35.0	34.0	40.0	29.0	41.0
36	34.6066809230888	35.0	34.0	40.0	27.0	41.0
37	34.49551819437473	35.0	34.0	40.0	27.0	41.0
38	34.45673192487854	35.0	34.0	40.0	27.0	41.0
39	34.48424673657046	35.0	34.0	40.0	27.0	41.0
40	34.27248033099477	35.0	34.0	40.0	26.0	41.0
41	34.225013170791854	35.0	34.0	40.0	26.0	41.0
42	34.20723914047973	35.0	34.0	40.0	26.0	41.0
43	34.10859146632325	35.0	34.0	39.0	26.0	41.0
44	33.98882132933456	35.0	34.0	39.0	25.0	41.0
45	33.87315250842868	35.0	34.0	39.0	24.0	41.0
46	33.89575396088991	35.0	34.0	39.0	25.0	41.0
47	33.83611300352591	35.0	34.0	39.0	25.0	41.0
48	33.82321496849596	35.0	34.0	39.0	24.0	41.0
49	33.830339650746716	35.0	34.0	39.0	24.0	41.0
50	33.536731875059935	35.0	34.0	38.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	1.0
13	3.0
14	12.0
15	24.0
16	82.0
17	229.0
18	538.0
19	1204.0
20	2248.0
21	3649.0
22	5642.0
23	8627.0
24	12007.0
25	17361.0
26	23535.0
27	28654.0
28	30447.0
29	31946.0
30	35942.0
31	42785.0
32	54501.0
33	75381.0
34	171532.0
35	436129.0
36	75540.0
37	85634.0
38	143874.0
39	318297.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.632443365595027	23.694659321744695	30.565142176051452	14.107755136608823
2	30.806886922991655	25.180312188244553	31.509204608718505	12.503596280045285
3	27.085811289641594	24.316893611138443	34.51108650625908	14.08620859296088
4	24.98464964448203	26.876261811678226	33.275398455374365	14.863690088465376
5	22.585261416865837	32.10179683228445	32.000976444521385	13.311965306328332
6	20.532361538547764	38.977448366136805	29.479657198226956	11.010532897088476
7	86.85143969520982	4.3820438827120745	7.378072094984139	1.388444327093969
8	89.47881028206045	2.6968675311023738	6.502759327598382	1.3215628592387967
9	87.1620586539264	3.304218514334679	7.644726140939305	1.8889966907996258
10	66.07228927667133	15.000130773819828	11.230295187647975	7.697284761860875
11	60.5204424389691	14.112736996411815	16.344361095162242	9.022459469456841
12	51.73051127581692	19.538729600840938	19.10368869354463	9.6270704297975
13	17.77384349238336	52.74033425788348	19.78221799871219	9.70360425102097
14	11.033823091667466	52.22284357084766	27.75325595674749	8.99007738073739
15	9.214946077594957	19.654371021517896	61.1649082777337	9.965774623153441
16	10.972733035833272	13.106276769712286	58.317962220066185	17.603027974388258
17	12.125161754760478	13.177704184637687	29.597727275557872	45.09940678504396
18	21.17944285370893	19.025535767885188	39.720741848743266	20.07427952966262
19	32.88500746656238	21.230444643442066	25.802421931143225	20.08212595885233
20	35.50583936242158	20.325116170743282	24.007582390620154	20.16146207621498
21	20.154425199243256	29.206277641537753	28.711392143358	21.927905015860997
22	20.93022531706424	23.38534810122641	24.768374655784626	30.916051925924727
23	15.956025123518986	31.988584068261446	24.79851490759273	27.25687590062684
24	17.107208377495446	20.2081047386205	47.67110508859615	15.013581795287909
25	12.648755220054975	22.19704999171766	44.75005386635912	20.404140921868247
26	11.98187101217691	39.80294253549263	29.23386469019682	18.981321762133632
27	15.160671205971257	37.80727177166144	30.47671416454834	16.555342857818967
28	11.018690692515877	28.01704543331594	47.0671791339784	13.897084740189783
29	11.13090708457828	23.69441022875455	44.5269288204326	20.647753866234574
30	16.063820115006234	32.916829096053995	34.00573910249305	17.013611686446726
31	34.491781799522485	24.801192657236836	24.06281876118583	16.644206782054844
32	35.689296349666776	24.403577971710508	26.295003319164096	13.61212235945862
33	30.993831212098943	25.98058569234774	24.65136322366184	18.374219871891476
34	17.538326070196895	28.378915274755794	27.90059446042099	26.182164194626317
35	18.50866781332473	24.49686329652154	34.422596221508435	22.571872668645295
36	36.273045772082405	21.723275124453085	26.02971928465475	15.97395981880976
37	19.024913035409813	35.73799402924103	29.486818621693757	15.750274313655401
38	18.646540783372544	36.968700220322745	22.944017595928823	21.44074140037588
39	19.818585575274035	34.48113307419359	27.326061478640902	18.374219871891476
40	26.147042083015222	25.356047292795107	23.645027543457388	24.851883080732286
41	15.386038088809123	22.094485953023554	27.50964301238117	35.00983294578616
42	23.27879857468991	22.12960806463465	23.215342135449298	31.37625122522614
43	22.681099944825903	24.945106132295777	24.83469566441196	27.53909825846636
44	18.336046371151046	31.093655227901408	29.92217089522775	20.6481275057198
45	14.312198208274122	45.36942358636614	20.141036451022714	20.177341754337018
46	21.926285911425023	35.60896386034352	24.13175524620974	18.332994982021713
47	20.244472315182342	28.051918451936885	26.221209520832268	25.482399712048505
48	23.49756449328881	22.373407828743588	34.177800085438896	19.951227592528706
49	20.972322032399525	22.239208980300482	35.446243864528284	21.34222512277171
50	19.138374892422966	37.10084405159712	25.431833835048128	18.32894722093178
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2454.0
1	3165.0
2	3876.0
3	9967.0
4	16058.0
5	11897.0
6	7736.0
7	8109.5
8	8483.0
9	8894.0
10	9305.0
11	9260.0
12	9215.0
13	8481.5
14	7748.0
15	7293.0
16	6838.0
17	6130.0
18	5422.0
19	5195.5
20	4969.0
21	4742.5
22	4516.0
23	5319.5
24	6123.0
25	6945.0
26	7767.0
27	8621.5
28	9476.0
29	11396.5
30	13317.0
31	16092.5
32	18868.0
33	20560.5
34	22253.0
35	27536.5
36	32820.0
37	33093.0
38	33366.0
39	54281.0
40	75196.0
41	119437.5
42	163679.0
43	197230.0
44	230781.0
45	223688.0
46	216595.0
47	186076.5
48	155558.0
49	143236.0
50	130914.0
51	121989.0
52	113064.0
53	100519.5
54	87975.0
55	75115.5
56	62256.0
57	53489.0
58	44722.0
59	37966.5
60	31211.0
61	26867.5
62	22524.0
63	19073.0
64	15622.0
65	12277.5
66	8933.0
67	7178.0
68	5423.0
69	4683.0
70	3943.0
71	3275.5
72	2608.0
73	2259.0
74	1910.0
75	1381.5
76	853.0
77	758.5
78	664.0
79	528.5
80	393.0
81	283.5
82	174.0
83	141.5
84	109.0
85	90.5
86	72.0
87	44.0
88	16.0
89	15.5
90	15.0
91	9.5
92	4.0
93	3.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1605826.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.296386740449925
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.95107042763867	10.76356739459283
2	7.226043044538216	1.9216052584663672
3	2.269763007652301	0.9053894027673542
4	1.1349860350094916	0.6036485306598416
5	0.6659646439977255	0.44274617310299064
6	0.48606699568530487	0.38777608538402514
7	0.35795048230847415	0.33316136326928364
8	0.28795605894203563	0.30630200991592804
9	0.2551804590077594	0.3053680264415428
>10	2.9462473612244096	9.294354752801517
>50	0.9996463206703934	9.784932526454524
>100	2.387412898417581	61.30292103947544
>500	0.02887236058752428	2.3350442923408394
>1k	0.002366586933403629	0.7209102328319629
>5k	4.7331738668072586E-4	0.5922729114955567
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9411	0.5860535325745131	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	4670	0.2908160659996787	No Hit
TTCGCCGGGGGAGGAGCTACTCTAGTATTAATAAATATTAGCCCACCAAC	2627	0.16359182128076144	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1936	0.12056100723241497	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.035122111611096095	0.0
2	0.0	0.0	0.0	0.1251692275501829	0.0
3	0.0	0.0	0.0	0.1815887898190713	0.0
4	0.0	0.0	0.0	0.28085234639369394	0.0
5	0.0	0.0	0.0	0.4712216641155393	0.0
6	0.0	0.0	0.0	0.6689392250467984	0.0
7	0.0	0.0	0.0	0.7820274425747248	0.0
8	0.0	0.0	0.0	1.0341095486061378	0.0
9	0.0	0.0	0.0	1.1216657346437284	0.0
10	0.0	0.0	0.0	1.2628391868110243	0.0
11	0.0	0.0	0.0	1.4169654744661002	0.0
12	0.0	0.0	0.0	1.535471464529781	0.0
13	0.0	0.0	0.0	1.5872828064809015	0.0
14	0.0	0.0	0.0	1.6050306820290616	0.0
15	0.0	0.0	0.0	1.6339254688864173	0.0
16	0.0	0.0	0.0	1.6978800941073318	0.0
17	0.0	0.0	0.0	1.7812639725599162	0.0
18	0.0	0.0	0.0	1.911788699398316	0.0
19	0.0	0.0	0.0	1.9650945992903341	0.0
20	0.0	0.0	0.0	2.020829155836311	0.0
21	0.0	0.0	0.0	2.101971197377549	0.0
22	0.0	0.0	0.0	2.174519530758625	0.0
23	0.0	0.0	0.0	2.2725998956300373	0.0
24	0.0	0.0	0.0	2.3447123162783514	0.0
25	0.0	0.0	0.0	2.3965859314770093	0.0
26	0.0	0.0	0.0	2.4619105681437468	0.0
27	0.0	0.0	0.0	2.5117291661736703	0.0
28	0.0	0.0	0.0	2.571324664066966	0.0
29	0.0	0.0	0.0	2.63552838227803	0.0
30	0.0	0.0	0.0	2.7282532478612254	0.0
31	0.0	0.0	0.0	2.825959973247413	0.0
32	6.227324753740442E-5	0.0	0.0	2.8950210047663942	0.0
33	6.227324753740442E-5	0.0	0.0	2.967195698662246	0.0
34	6.227324753740442E-5	0.0	0.0	3.043044514162805	0.0
35	6.227324753740442E-5	0.0	0.0	3.1538908947793844	0.0
36	6.227324753740442E-5	0.0	0.0	3.2342856573501737	0.0
37	6.227324753740442E-5	0.0	0.0	3.314867239663575	0.0
38	6.227324753740442E-5	0.0	0.0	3.3884119450052497	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGTA	40	8.3164196E-9	44.0	33
AACGTAG	45	4.802132E-10	44.0	1
TCGTTAG	145	0.0	44.0	1
GGTACAA	35	1.4465695E-7	44.0	8
AATCATC	20	7.8574184E-4	44.0	18
TCACCGT	40	8.3164196E-9	44.0	26
CGTATCG	25	4.443633E-5	44.0	12
CGAGTAC	40	8.3164196E-9	44.0	41
CGACGGT	165	0.0	44.0	28
CGTGCCT	25	4.443633E-5	44.0	16
CGTGCCG	20	7.8574184E-4	44.0	41
TTAGCCG	25	4.443633E-5	44.0	3
TAGACGT	25	4.443633E-5	44.0	39
TAGACCG	25	4.443633E-5	44.0	1
CGGCCCA	20	7.8574184E-4	44.0	20
CACATCG	30	2.528348E-6	44.0	17
CTATACG	70	0.0	44.0	1
CGTTGCG	20	7.8574184E-4	44.0	36
GGCGTAA	25	4.443633E-5	44.0	1
CGCATAA	40	8.3164196E-9	44.0	18
>>END_MODULE