FastQCFastQC Report
Thu 26 May 2016
SRR1545893_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545893_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2989082
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT476421.5938672809912877No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA35500.11876556079759605No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCG254.4445558E-544.01
CGCGGAC254.4445558E-544.032
GTATCGC254.4445558E-544.025
GACTACG207.858507E-444.01
TACGTCA650.043.99999639
CGTTTTT333700.043.3275381
CGACGGT2000.041.80000328
GCGTAAG2650.041.5094381
GCGCGAC1300.040.6153839
CTATCGA452.3530447E-839.1111126
CACGACG2200.039.026
AGTACGG4800.038.9583322
TACGGGA16000.038.9125024
CGTAAGG5050.038.7722782
TAACGCG800.038.51
TCGTCGG404.127487E-738.52
CGTTTGG2750.038.42
ACGGGAT18300.038.229515
GCGATAT3700.038.0540549
CTTGCGG2550.037.9607852
Produced by FastQC (version 0.11.5)