##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545893_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2989082
Sequences flagged as poor quality	0
Sequence length	50
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.4429563993226	31.0	31.0	33.0	30.0	34.0
2	31.839959559490172	31.0	31.0	34.0	30.0	34.0
3	31.796492367890878	31.0	31.0	34.0	30.0	34.0
4	35.59423093779294	37.0	35.0	37.0	33.0	37.0
5	35.545320268898614	37.0	35.0	37.0	33.0	37.0
6	35.604427379376006	37.0	35.0	37.0	33.0	37.0
7	36.00234787804416	37.0	35.0	37.0	35.0	37.0
8	36.00340238240369	37.0	35.0	37.0	35.0	37.0
9	37.77562341882892	39.0	38.0	39.0	35.0	39.0
10	37.24347542155083	39.0	37.0	39.0	33.0	39.0
11	36.871179512639664	39.0	37.0	39.0	32.0	39.0
12	36.544637450561744	39.0	35.0	39.0	32.0	39.0
13	36.40066448494889	39.0	35.0	39.0	32.0	39.0
14	37.52259188607071	40.0	36.0	41.0	32.0	41.0
15	37.61500186344838	40.0	36.0	41.0	32.0	41.0
16	37.69751649503092	40.0	35.0	41.0	33.0	41.0
17	37.60113104959984	40.0	35.0	41.0	32.0	41.0
18	37.39651705774549	39.0	36.0	41.0	32.0	41.0
19	37.216937507903765	38.0	36.0	41.0	32.0	41.0
20	36.96432817835041	38.0	35.0	41.0	32.0	41.0
21	36.83672679438035	38.0	35.0	41.0	32.0	41.0
22	36.73821293627943	38.0	35.0	41.0	32.0	41.0
23	36.6340943473615	38.0	35.0	40.0	31.0	41.0
24	36.67145665458492	38.0	35.0	41.0	32.0	41.0
25	36.649320426806625	38.0	35.0	41.0	31.0	41.0
26	36.40395847286893	38.0	35.0	40.0	31.0	41.0
27	36.393842323495974	38.0	35.0	40.0	31.0	41.0
28	36.37177166768928	38.0	35.0	40.0	31.0	41.0
29	36.31137385993426	38.0	35.0	40.0	31.0	41.0
30	36.14328245260585	38.0	35.0	40.0	30.0	41.0
31	35.93045155669868	38.0	35.0	40.0	30.0	41.0
32	35.605419991823574	38.0	35.0	40.0	28.0	41.0
33	35.186689425047554	38.0	34.0	40.0	25.0	41.0
34	34.76761493997154	38.0	34.0	40.0	23.0	41.0
35	34.38828008064014	38.0	34.0	40.0	21.0	41.0
36	34.1898673238138	38.0	33.0	40.0	18.0	41.0
37	34.07680217538361	38.0	33.0	40.0	18.0	41.0
38	33.96626121330897	38.0	33.0	40.0	18.0	41.0
39	33.82809939640331	38.0	33.0	40.0	18.0	41.0
40	33.71834462888606	38.0	33.0	40.0	17.0	41.0
41	33.66244218124494	37.0	33.0	40.0	15.0	41.0
42	33.632838108824046	37.0	33.0	40.0	15.0	41.0
43	33.53525831676749	37.0	33.0	40.0	15.0	41.0
44	33.40169523619626	37.0	33.0	40.0	15.0	41.0
45	33.301291500199724	37.0	33.0	40.0	15.0	41.0
46	33.23281428880171	37.0	32.0	40.0	15.0	41.0
47	33.158593842524226	37.0	32.0	40.0	15.0	41.0
48	33.02678113213354	36.0	32.0	40.0	13.0	41.0
49	32.96101813198835	36.0	32.0	40.0	13.0	41.0
50	32.82178642138289	36.0	32.0	40.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	5.0
13	4.0
14	23.0
15	67.0
16	201.0
17	639.0
18	1499.0
19	2977.0
20	5286.0
21	8656.0
22	13234.0
23	20139.0
24	30506.0
25	48583.0
26	73450.0
27	88105.0
28	87369.0
29	81432.0
30	82089.0
31	89804.0
32	105360.0
33	130276.0
34	203110.0
35	306199.0
36	201718.0
37	257244.0
38	404179.0
39	746926.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.630422986053908	23.011212138041042	29.52555333042051	19.83281154548453
2	36.171339561778495	23.84809115306974	27.94142147990587	12.039147805245893
3	26.073623942066494	23.13804037493786	38.459299544140976	12.329036138854672
4	22.717944840589855	25.65142073720293	38.501954780765466	13.128679641441753
5	21.26539184940393	29.811861969661592	36.619370094229595	12.303376086704882
6	18.99897694342276	38.19507126268199	33.11200562580752	9.693946168087727
7	81.78153024908652	3.009720041136376	13.973387146956826	1.2353625628202907
8	83.11019905107989	1.9434394907868036	13.536028787433734	1.410332670699566
9	78.31210384994456	5.160279978936677	14.765871260808503	1.7617449103102558
10	36.624321447186794	37.613989847050036	18.50715370137052	7.254535004392652
11	27.990165542464208	21.356356232448626	36.054112935008135	14.599365290079028
12	26.764571865208115	21.25083888632028	36.47340554725498	15.511183701216627
13	19.54687091220649	28.62484200834905	37.15883338095107	14.669453698493385
14	15.563039086916985	31.196233492423424	37.135548640017234	16.10517878064235
15	14.8894543542131	24.756062229139246	45.578642539749666	14.775840876897991
16	18.080434059687892	23.28209798192221	42.46919288263085	16.168275075759045
17	18.27427283694459	22.18691223593063	36.22747719868508	23.311337728439703
18	19.545064337478866	23.649334477943395	38.80669717324583	17.998904011331906
19	22.37312325322624	24.05992876742759	35.01981544835505	18.54713253099112
20	23.451481090180863	24.88218790919754	34.63923037240196	17.027100628219635
21	21.39108261332409	24.558710667689944	36.981287231330555	17.068919487655407
22	20.789593594287478	21.776351401533983	36.285287589969094	21.148767414209445
23	17.92667447731444	25.68159722617178	35.89791113124364	20.49381716527014
24	17.377308484678576	24.236738905122042	40.89964745028741	17.48630515991197
25	17.227697333161153	26.12240145971238	38.02083716672878	18.629064040397687
26	18.075281976205403	28.904727270780796	34.83410625737267	18.185884495641137
27	17.407752614347814	28.500087986880253	35.93976344576696	18.15239595300497
28	16.02993828874551	26.381109651725847	40.49293395095886	17.096018108569787
29	17.518187858345808	23.188992473274403	39.36971284160153	19.923106826778255
30	18.752914774502674	25.911567497980986	37.32945432744903	18.006063400067312
31	22.413436633722327	25.074353932076804	33.91211749962029	18.600091934580583
32	23.111242849811415	25.618835481930574	34.493500011040176	16.776421657217835
33	22.152821501718588	26.39532806393401	33.0519202885702	18.399930145777198
34	18.844682079648535	27.48151439137501	33.99729415251907	19.67650937645739
35	19.118578881409075	27.176203262406318	33.519287861624406	20.185929994560205
36	23.318965488400785	26.945630799021238	31.098578091869012	18.636825620708965
37	19.34657530305291	30.115165793377365	32.363013125769044	18.175245777800676
38	19.226070077702786	30.01068555496303	30.505118293844063	20.25812607349012
39	19.939466364589528	29.872817139175172	30.94846511403836	19.239251382196944
40	21.46692529679681	27.70201687340796	30.234567000838386	20.596490828956853
41	18.91600832630219	27.386401577474288	30.16374258049796	23.533847515725565
42	20.78122982240032	27.36575978845679	29.450613934311605	22.402396454831283
43	20.796853348285527	27.04991030691028	30.830870481304963	21.32236586349923
44	19.577783413101415	28.99632730048891	30.79453825622716	20.63135103018251
45	18.89319195659403	30.831372307618192	29.54923953240493	20.726196203382845
46	20.824353430250493	29.339676864000385	30.103523422910445	19.73244628283868
47	20.171644672176942	27.33621894615136	31.38023647394083	21.11189990773087
48	20.72027465288674	26.77868991215363	31.588628214281172	20.912407220678457
49	20.44416981534799	25.89938984611329	32.739784321741595	20.91665601679713
50	19.092985739434383	29.439975216471144	31.252906410730784	20.214132633363686
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	821.0
1	2617.5
2	4414.0
3	44325.5
4	84237.0
5	60954.5
6	37672.0
7	37248.0
8	36824.0
9	36908.5
10	36993.0
11	36307.5
12	35622.0
13	34067.0
14	32512.0
15	30424.0
16	28336.0
17	26570.5
18	24805.0
19	23654.5
20	22504.0
21	22478.5
22	22453.0
23	23170.5
24	23888.0
25	27488.0
26	31088.0
27	34544.0
28	38000.0
29	46117.0
30	54234.0
31	64801.5
32	75369.0
33	86823.5
34	98278.0
35	104223.0
36	110168.0
37	119121.0
38	128074.0
39	143442.5
40	158811.0
41	187827.0
42	216843.0
43	242393.0
44	267943.0
45	268267.0
46	268591.0
47	253826.5
48	239062.0
49	218631.5
50	198201.0
51	184130.0
52	170059.0
53	154244.5
54	138430.0
55	123538.0
56	108646.0
57	99653.5
58	90661.0
59	80746.5
60	70832.0
61	61324.0
62	51816.0
63	42891.0
64	33966.0
65	26963.0
66	19960.0
67	15710.0
68	11460.0
69	9451.5
70	7443.0
71	5961.5
72	4480.0
73	3765.0
74	3050.0
75	2161.5
76	1273.0
77	961.5
78	650.0
79	503.5
80	357.0
81	254.0
82	151.0
83	101.0
84	51.0
85	37.0
86	23.0
87	17.5
88	12.0
89	11.0
90	10.0
91	8.0
92	6.0
93	3.0
94	0.0
95	1.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2989082.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.303531239742906
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.18179504452829	17.660336393945567
2	8.200335362179988	3.6579287185349965
3	2.892891850302138	1.9356511126923417
4	1.4496438170149941	1.2932870463717627
5	0.8995917401328098	1.0032036239533402
6	0.5925652116036622	0.7929778025152286
7	0.47346910110176277	0.739202302123331
8	0.3560166232567291	0.635234230293939
9	0.315353506672312	0.6330147108865545
>10	3.5253905742661455	18.548875146758697
>50	1.1940384789805958	19.131916322280997
>100	0.9146290970115631	31.532964945743974
>500	0.003362537317172174	0.5135211884843444
>1k	7.642130266300396E-4	0.29780410930690604
>5k	0.0	0.0
>10k+	1.5284260532600792E-4	1.6240823461080147
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	47642	1.5938672809912877	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3550	0.11876556079759605	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06908475578789743	0.0
2	0.0	0.0	0.0	0.24355303735394346	0.0
3	0.0	0.0	0.0	0.34706307822936944	0.0
4	0.0	0.0	0.0	0.4894144757487416	0.0
5	0.0	0.0	0.0	0.8204860221298713	0.0
6	0.0	0.0	0.0	1.1193068641141326	0.0
7	0.0	0.0	0.0	1.3190337367793858	0.0
8	0.0	0.0	0.0	1.794631261370548	0.0
9	0.0	0.0	0.0	1.9901427930046751	0.0
10	0.0	0.0	0.0	2.318236836593978	0.0
11	0.0	0.0	0.0	2.6856406080529074	0.0
12	0.0	0.0	0.0	3.0061403467686736	0.0
13	0.0	0.0	0.0	3.1255415542296934	0.0
14	0.0	0.0	0.0	3.1712077487335577	0.0
15	0.0	0.0	0.0	3.243203097138185	0.0
16	0.0	0.0	0.0	3.4070995710388674	0.0
17	0.0	0.0	0.0	3.6336574239181125	0.0
18	0.0	0.0	0.0	3.931407703100818	0.0
19	0.0	0.0	0.0	4.095973278752473	0.0
20	0.0	0.0	0.0	4.266594225250428	0.0
21	0.0	0.0	0.0	4.469766971933189	0.0
22	0.0	0.0	0.0	4.711714165084799	0.0
23	0.0	0.0	0.0	4.969251429034065	0.0
24	0.0	0.0	0.0	5.162488014714886	0.0
25	0.0	0.0	0.0	5.3228382493354145	0.0
26	0.0	0.0	0.0	5.480010250638825	0.0
27	0.0	0.0	0.0	5.642568521037563	0.0
28	0.0	0.0	0.0	5.828177346757299	0.0
29	0.0	0.0	0.0	6.020979016299988	0.0
30	0.0	0.0	0.0	6.266438993644202	0.0
31	0.0	0.0	0.0	6.486841110414502	0.0
32	0.0	0.0	0.0	6.6867018034299495	0.0
33	0.0	0.0	0.0	6.885993759957071	0.0
34	0.0	0.0	0.0	7.096894631863562	0.0
35	0.0	0.0	0.0	7.338708004664977	0.0
36	0.0	0.0	0.0	7.5615857979138745	0.0
37	0.0	0.0	0.0	7.794567027602454	0.0
38	0.0	0.0	0.0	8.035544023215154	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACGCG	25	4.4445558E-5	44.0	1
CGCGGAC	25	4.4445558E-5	44.0	32
GTATCGC	25	4.4445558E-5	44.0	25
GACTACG	20	7.858507E-4	44.0	1
TACGTCA	65	0.0	43.999996	39
CGTTTTT	33370	0.0	43.327538	1
CGACGGT	200	0.0	41.800003	28
GCGTAAG	265	0.0	41.509438	1
GCGCGAC	130	0.0	40.615383	9
CTATCGA	45	2.3530447E-8	39.11111	26
CACGACG	220	0.0	39.0	26
AGTACGG	480	0.0	38.958332	2
TACGGGA	1600	0.0	38.912502	4
CGTAAGG	505	0.0	38.772278	2
TAACGCG	80	0.0	38.5	1
TCGTCGG	40	4.127487E-7	38.5	2
CGTTTGG	275	0.0	38.4	2
ACGGGAT	1830	0.0	38.22951	5
GCGATAT	370	0.0	38.054054	9
CTTGCGG	255	0.0	37.960785	2
>>END_MODULE