FastQCFastQC Report
Thu 26 May 2016
SRR1545892_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545892_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1632027
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203351.2459965429493507No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA32450.19883249480553938No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGG2150.044.02
ACCGCAA207.8574545E-444.029
CGGCATA254.4436638E-544.019
CGTGACC207.8574545E-444.030
CGCATGT302.5283734E-644.035
TAGCGAT207.8574545E-444.030
CAACCCG502.7284841E-1144.013
ATTAACG454.802132E-1044.01
TAAGTCG254.4436638E-544.02
TACGCGC207.8574545E-444.011
TCGGAAT302.5283734E-644.023
TACGATA207.8574545E-444.033
TAATTCG408.3164196E-944.036
CTACGCC207.8574545E-444.042
CTACCGT302.5283734E-644.022
ACGTCAA207.8574545E-444.010
CGTAATC408.3164196E-944.034
TTAGTCG254.4436638E-544.038
CTCGAAT254.4436638E-544.010
ACGTATA207.8574545E-444.025
Produced by FastQC (version 0.11.5)