##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545892_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1632027
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.581385602076438	31.0	31.0	33.0	30.0	34.0
2	31.981083033552753	31.0	31.0	34.0	30.0	34.0
3	31.998640953856768	33.0	31.0	34.0	30.0	34.0
4	35.75989858010927	37.0	35.0	37.0	35.0	37.0
5	35.692059016180494	37.0	35.0	37.0	33.0	37.0
6	35.74293011083763	37.0	35.0	37.0	33.0	37.0
7	36.052703172190164	37.0	35.0	37.0	35.0	37.0
8	36.04376643278573	37.0	35.0	37.0	35.0	37.0
9	37.839295550870176	39.0	38.0	39.0	35.0	39.0
10	37.38321486102865	39.0	37.0	39.0	35.0	39.0
11	37.11547174158271	39.0	37.0	39.0	33.0	39.0
12	36.294335204013166	38.0	35.0	39.0	33.0	39.0
13	35.92929344918926	38.0	35.0	39.0	32.0	39.0
14	36.93934230254769	39.0	35.0	41.0	32.0	41.0
15	37.16460818356559	39.0	35.0	41.0	32.0	41.0
16	37.32840694424786	39.0	35.0	41.0	33.0	41.0
17	37.268283551681435	39.0	35.0	41.0	33.0	41.0
18	37.12104456605191	38.0	35.0	40.0	32.0	41.0
19	36.96034563153673	38.0	35.0	40.0	32.0	41.0
20	36.714303133465314	38.0	35.0	40.0	32.0	41.0
21	36.55894847327893	37.0	35.0	40.0	32.0	41.0
22	36.46140229297677	37.0	35.0	40.0	32.0	41.0
23	36.396994044828915	37.0	35.0	40.0	32.0	41.0
24	36.42798311547542	37.0	35.0	40.0	32.0	41.0
25	36.40498165777895	37.0	35.0	40.0	32.0	41.0
26	36.167429215325484	37.0	35.0	40.0	31.0	41.0
27	36.14031936971631	37.0	35.0	40.0	31.0	41.0
28	36.14198049419525	37.0	35.0	40.0	31.0	41.0
29	36.12930913520426	37.0	35.0	40.0	31.0	41.0
30	36.0163067155139	36.0	35.0	40.0	31.0	41.0
31	35.79413882245821	36.0	35.0	40.0	30.0	41.0
32	35.50392119738215	37.0	35.0	40.0	30.0	41.0
33	35.166841602497996	36.0	34.0	40.0	27.0	41.0
34	34.84831623496425	36.0	34.0	40.0	25.0	41.0
35	34.56060959775788	36.0	34.0	40.0	23.0	41.0
36	34.34970806242789	36.0	34.0	40.0	23.0	41.0
37	34.263518924625636	36.0	34.0	40.0	23.0	41.0
38	34.18760290117749	36.0	33.0	40.0	23.0	41.0
39	34.10802946274786	36.0	33.0	40.0	22.0	41.0
40	33.927854747501115	36.0	33.0	40.0	21.0	41.0
41	33.92817153147589	36.0	33.0	40.0	21.0	41.0
42	33.90840041249317	36.0	33.0	40.0	21.0	41.0
43	33.78556849856038	35.0	33.0	40.0	20.0	41.0
44	33.62819916582262	35.0	33.0	40.0	19.0	41.0
45	33.54454613802345	35.0	33.0	40.0	20.0	41.0
46	33.52389022975723	35.0	33.0	40.0	20.0	41.0
47	33.48599624883657	35.0	33.0	40.0	20.0	41.0
48	33.41139147820471	35.0	33.0	40.0	20.0	41.0
49	33.36844794847144	35.0	33.0	40.0	19.0	41.0
50	33.167138778954026	35.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	4.0
13	2.0
14	9.0
15	27.0
16	86.0
17	242.0
18	627.0
19	1238.0
20	2319.0
21	3842.0
22	5917.0
23	9010.0
24	13846.0
25	21591.0
26	33056.0
27	41400.0
28	42314.0
29	41324.0
30	43046.0
31	49163.0
32	59518.0
33	77642.0
34	141732.0
35	252273.0
36	103514.0
37	125061.0
38	197202.0
39	366021.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.699247010006577	21.65013201374732	28.985917512394092	18.66470346385201
2	33.879035089493	24.34175414990071	29.13444446691139	12.644766293694895
3	26.610466616054758	23.533434189507894	37.08970501100779	12.766394183429563
4	24.756453171424248	26.31365780100452	35.053831829988106	13.876057197583128
5	22.30894464368543	30.32082189816713	34.20317188379849	13.167061574348955
6	19.52461570795091	39.85742882930246	30.706906197017574	9.911049265729059
7	83.1557321049223	3.6218763537613046	11.66218451042783	1.560207030888582
8	84.44412990716452	2.575508861066637	11.161825141373274	1.8185360903955634
9	79.774109129322	5.437716410328996	12.663822350978263	2.1243521093707396
10	44.49981526040929	30.73913605595986	15.974551891604735	8.786496792026112
11	43.04536628376859	18.292528248613536	26.1764664432635	12.485639024354377
12	38.6762596452142	19.78551825429359	28.021350136976903	13.516871963515309
13	21.04793609419452	35.7014926836382	29.16508121495539	14.08549000721189
14	14.275192751100318	39.05339801363581	32.11117218036221	14.560237054901664
15	14.183037412983976	21.767286938267567	49.9839157072769	14.065759941471557
16	16.052369231636487	17.650627103595713	48.471134362360424	17.825869302407373
17	17.008235770609186	18.03646630846181	32.88983576864843	32.065462152280574
18	20.247704235285326	21.93701452243131	37.68516084599091	20.130120396292465
19	27.399730519164205	21.661896524996216	31.883602415891403	19.054770539948173
20	29.394856825285366	21.57991258723048	29.589032534388217	19.436198053095936
21	22.306432430345822	25.574883258671576	32.33586209051688	19.782822220465714
22	21.26055512561986	22.187806941919465	30.813460806714595	25.73817712574608
23	18.628797195144443	27.240603249823685	30.915236083716753	23.215363471315122
24	18.761454314174948	21.854540396696866	42.19250049171981	17.191504797408378
25	16.81301841207284	23.59446259161154	38.784468639305594	20.808050357010025
26	16.99040518324758	32.02183542306592	31.73378871795626	19.25397067573024
27	17.63916895982726	31.315597107155703	33.511210292476775	17.534023640540262
28	15.397110464471483	26.098097641766955	42.18514767218925	16.319644221572315
29	16.52717755282235	22.45734905121055	40.68400829152949	20.33146510443761
30	18.033463907153497	27.75818047127897	35.49953524053217	18.708820381035363
31	26.71144533760777	23.805856153115116	30.310282856839994	19.17241565243712
32	27.995921636100384	24.83218721258901	30.966215632461964	16.205675518848647
33	26.57627600523766	25.881434559599807	29.291120796408393	18.25116863875414
34	19.679576379557446	26.460898012103968	31.756705005493167	22.10282060284542
35	19.938885815001836	25.377643874764328	33.13860616276569	21.54486414746815
36	27.593293493306177	24.230297660516644	29.919296678302505	18.257112167874674
37	20.01872518040449	31.130612422466047	30.71481047801293	18.135851919116533
38	20.275461129013184	31.085331308857022	27.425833028497692	21.213374533632106
39	20.573127773008657	28.948173038803894	29.9534872891196	20.52521189906785
40	22.902807367770265	24.806819985208577	29.318387502167546	22.97198514485361
41	17.00106677156689	24.947994120195315	29.77230156118741	28.278637547050383
42	21.78946794385142	25.333526957580972	27.476016021793757	25.40098907677385
43	22.85342092992334	26.07959304594838	27.81700302752344	23.249982996604835
44	19.763521069198	30.17535861845423	29.513972501680424	20.547147810667347
45	17.654854974825785	35.105485387190285	25.70882712111993	21.530832516864
46	20.729375188033043	30.925223663579093	28.77679107024577	19.56861007814209
47	21.0766120903637	26.347603317837265	29.75061074357226	22.825173848226775
48	22.517642171361135	23.337787916498932	32.438495196464274	21.706074715675662
49	21.120177546082264	23.16040114532419	33.361948056006426	22.35747325258712
50	18.637130390612413	31.159165871643058	29.078746858967406	21.12495687877713
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	593.0
1	1511.5
2	2430.0
3	19178.0
4	35926.0
5	25812.5
6	15699.0
7	15793.5
8	15888.0
9	16463.0
10	17038.0
11	16761.0
12	16484.0
13	15126.5
14	13769.0
15	12708.5
16	11648.0
17	10520.0
18	9392.0
19	8666.0
20	7940.0
21	7715.0
22	7490.0
23	8407.0
24	9324.0
25	9882.5
26	10441.0
27	12317.0
28	14193.0
29	17741.5
30	21290.0
31	25337.0
32	29384.0
33	32560.0
34	35736.0
35	39029.5
36	42323.0
37	46048.5
38	49774.0
39	62882.0
40	75990.0
41	102424.5
42	128859.0
43	144598.5
44	160338.0
45	165653.0
46	170968.0
47	155333.5
48	139699.0
49	128468.0
50	117237.0
51	108558.0
52	99879.0
53	90548.5
54	81218.0
55	72108.0
56	62998.0
57	58836.0
58	54674.0
59	54758.0
60	54842.0
61	47443.0
62	40044.0
63	34166.5
64	28289.0
65	24545.5
66	20802.0
67	15829.5
68	10857.0
69	8783.5
70	6710.0
71	5752.0
72	4794.0
73	4099.5
74	3405.0
75	2546.0
76	1687.0
77	1267.0
78	847.0
79	738.5
80	630.0
81	420.0
82	210.0
83	141.0
84	72.0
85	59.5
86	47.0
87	77.0
88	107.0
89	73.5
90	40.0
91	30.0
92	20.0
93	10.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1632027.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.577457499184064
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.23422609300253	14.905479252226797
2	8.030031794286462	2.9835514875090703
3	2.652341496597831	1.4782128427910541
4	1.290753763847128	0.9591569275912754
5	0.7504221890513602	0.6970468161773156
6	0.5212972226422837	0.5810626198847831
7	0.3940142828337804	0.5123848515391232
8	0.2911405221316914	0.43269205409534034
9	0.2673893297466727	0.44706725181937124
>10	2.9568948907459847	13.160913247602114
>50	1.1635543002069537	15.714050494573126
>100	1.4399234144098372	45.74344250914665
>500	0.007009362935235579	0.8539078532551649
>1k	6.675583747843409E-4	0.27010548317329885
>5k	0.0	0.0
>10k+	3.3377918739217044E-4	1.2609263086154803
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20335	1.2459965429493507	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3245	0.19883249480553938	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07211890489556852	0.0
2	0.0	0.0	0.0	0.24276559150063082	0.0
3	0.0	0.0	0.0	0.3341243741678293	0.0
4	0.0	0.0	0.0	0.48265132868512595	0.0
5	0.0	0.0	0.0	0.7882835271720382	0.0
6	0.0	0.0	0.0	1.0836830518122555	0.0
7	0.0	0.0	0.0	1.2665844376349167	0.0
8	0.0	0.0	0.0	1.7875317013750385	0.0
9	0.0	0.0	0.0	1.9964130495390089	0.0
10	0.0	0.0	0.0	2.3928525692283276	0.0
11	0.0	0.0	0.0	2.6882520938685452	0.0
12	0.0	0.0	0.0	3.0014822058703685	0.0
13	0.0	0.0	0.0	3.1190660448632284	0.0
14	0.0	0.0	0.0	3.158832543824336	0.0
15	0.0	0.0	0.0	3.2232922617089055	0.0
16	0.0	0.0	0.0	3.3773338308741216	0.0
17	0.0	0.0	0.0	3.577146701617069	0.0
18	0.0	0.0	0.0	3.8795926783074055	0.0
19	0.0	0.0	0.0	4.002629858452097	0.0
20	0.0	0.0	0.0	4.131610567717323	0.0
21	0.0	0.0	0.0	4.295884810729234	0.0
22	0.0	0.0	0.0	4.467205505791265	0.0
23	0.0	0.0	0.0	4.6535382073948535	0.0
24	0.0	0.0	0.0	4.789565368710199	0.0
25	0.0	0.0	0.0	4.906291378757827	0.0
26	0.0	0.0	0.0	5.0271839865394385	0.0
27	0.0	0.0	0.0	5.138211561450883	0.0
28	6.127349608799364E-5	0.0	0.0	5.255182665482863	0.0
29	6.127349608799364E-5	0.0	0.0	5.389800536388185	0.0
30	6.127349608799364E-5	0.0	0.0	5.560998684458039	0.0
31	6.127349608799364E-5	0.0	0.0	5.726069482919094	0.0
32	6.127349608799364E-5	0.0	0.0	5.8717165831202545	0.0
33	6.127349608799364E-5	0.0	0.0	6.006395727521665	0.0
34	6.127349608799364E-5	0.0	0.0	6.150265896336274	0.0
35	6.127349608799364E-5	0.0	0.0	6.332432000205879	0.0
36	6.127349608799364E-5	0.0	0.0	6.494622944350798	0.0
37	6.127349608799364E-5	0.0	0.0	6.6565075210152775	0.0
38	6.127349608799364E-5	0.0	0.0	6.812019654086606	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAAAGG	215	0.0	44.0	2
ACCGCAA	20	7.8574545E-4	44.0	29
CGGCATA	25	4.4436638E-5	44.0	19
CGTGACC	20	7.8574545E-4	44.0	30
CGCATGT	30	2.5283734E-6	44.0	35
TAGCGAT	20	7.8574545E-4	44.0	30
CAACCCG	50	2.7284841E-11	44.0	13
ATTAACG	45	4.802132E-10	44.0	1
TAAGTCG	25	4.4436638E-5	44.0	2
TACGCGC	20	7.8574545E-4	44.0	11
TCGGAAT	30	2.5283734E-6	44.0	23
TACGATA	20	7.8574545E-4	44.0	33
TAATTCG	40	8.3164196E-9	44.0	36
CTACGCC	20	7.8574545E-4	44.0	42
CTACCGT	30	2.5283734E-6	44.0	22
ACGTCAA	20	7.8574545E-4	44.0	10
CGTAATC	40	8.3164196E-9	44.0	34
TTAGTCG	25	4.4436638E-5	44.0	38
CTCGAAT	25	4.4436638E-5	44.0	10
ACGTATA	20	7.8574545E-4	44.0	25
>>END_MODULE