FastQCFastQC Report
Thu 26 May 2016
SRR1545891_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545891_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2761857
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT361601.3092640205484931No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA64780.23455233199981027No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGCT302.5290137E-644.044
GTCGATA351.4470788E-744.027
CTATCGA207.858404E-444.031
TAAGTCG502.7284841E-1144.01
TTAACGC254.4444685E-544.013
CTACCGA351.4470788E-744.012
ACGTAAC207.858404E-444.016
GTACGTC302.5290137E-644.025
CCGGATA950.043.99999620
CGTTTTT240350.042.8466841
CGGTCTA3400.042.05882631
GCTACGA2000.041.810
TAATGCG1700.041.4117661
AAACGCG750.041.0666661
CGACGGT3600.040.94444728
ACGGTCT3550.040.9014130
ATAGCGG4700.040.7234042
CGCGTCA650.040.61538739
ACGGGTA2250.040.088895
AATACGG1950.039.487182
Produced by FastQC (version 0.11.5)