##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545891_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2761857
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.5481308409523	31.0	31.0	33.0	30.0	34.0
2	31.945353796376857	31.0	31.0	34.0	30.0	34.0
3	31.963678785686586	33.0	31.0	34.0	30.0	34.0
4	35.726491994335696	37.0	35.0	37.0	33.0	37.0
5	35.66529983268504	37.0	35.0	37.0	33.0	37.0
6	35.71696398473925	37.0	35.0	37.0	33.0	37.0
7	36.028914603471506	37.0	35.0	37.0	35.0	37.0
8	36.03101645016378	37.0	35.0	37.0	35.0	37.0
9	37.80927289139155	39.0	38.0	39.0	35.0	39.0
10	37.33069923605748	39.0	37.0	39.0	34.0	39.0
11	37.05605757285768	39.0	37.0	39.0	33.0	39.0
12	36.435837554225294	38.0	35.0	39.0	33.0	39.0
13	36.16097357683616	38.0	35.0	39.0	32.0	39.0
14	37.23414934227225	39.0	35.0	41.0	32.0	41.0
15	37.39070451511429	39.0	35.0	41.0	32.0	41.0
16	37.524582554419	39.0	35.0	41.0	33.0	41.0
17	37.448170560604694	39.0	35.0	41.0	33.0	41.0
18	37.29725000244401	39.0	35.0	41.0	32.0	41.0
19	37.143330737253955	38.0	35.0	41.0	32.0	41.0
20	36.88330713719066	38.0	35.0	41.0	32.0	41.0
21	36.752049074227955	38.0	35.0	41.0	32.0	41.0
22	36.65172563242775	38.0	35.0	41.0	32.0	41.0
23	36.59592368468027	38.0	35.0	40.0	32.0	41.0
24	36.63724189920043	38.0	35.0	41.0	32.0	41.0
25	36.606220742058696	38.0	35.0	41.0	32.0	41.0
26	36.36087567169481	38.0	35.0	40.0	31.0	41.0
27	36.33346802531775	38.0	35.0	40.0	31.0	41.0
28	36.31745959330986	38.0	35.0	40.0	31.0	41.0
29	36.2792588464935	38.0	35.0	40.0	31.0	41.0
30	36.16118647706959	38.0	35.0	40.0	31.0	41.0
31	35.955675836945936	38.0	35.0	40.0	30.0	41.0
32	35.678432301165486	38.0	35.0	40.0	29.0	41.0
33	35.35316129691002	38.0	35.0	40.0	27.0	41.0
34	35.01887498157942	38.0	34.0	40.0	25.0	41.0
35	34.72601296881048	37.0	34.0	40.0	23.0	41.0
36	34.532285342796534	37.0	34.0	40.0	23.0	41.0
37	34.43428244112566	37.0	34.0	40.0	22.0	41.0
38	34.35444231906286	37.0	34.0	40.0	23.0	41.0
39	34.2356483337117	37.0	34.0	40.0	22.0	41.0
40	34.09437092506962	37.0	33.0	40.0	21.0	41.0
41	34.057644910652506	37.0	33.0	40.0	20.0	41.0
42	34.04266766889089	37.0	33.0	40.0	20.0	41.0
43	33.94319184519691	37.0	33.0	40.0	20.0	41.0
44	33.81392736843363	36.0	33.0	40.0	18.0	41.0
45	33.73197888232446	36.0	33.0	40.0	20.0	41.0
46	33.67078491029767	36.0	33.0	40.0	20.0	41.0
47	33.63676902895407	36.0	33.0	40.0	20.0	41.0
48	33.5373055158178	36.0	33.0	40.0	19.0	41.0
49	33.4613750820553	36.0	33.0	40.0	18.0	41.0
50	33.31312844944543	35.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	0.0
11	8.0
12	4.0
13	6.0
14	16.0
15	55.0
16	188.0
17	502.0
18	1172.0
19	2417.0
20	4162.0
21	6847.0
22	10734.0
23	16008.0
24	23933.0
25	37001.0
26	55937.0
27	70151.0
28	72067.0
29	69537.0
30	73039.0
31	81365.0
32	97141.0
33	124948.0
34	217929.0
35	359175.0
36	177755.0
37	223581.0
38	354412.0
39	681764.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.4116034972122	22.25086237267172	28.435686568855665	18.901847561260414
2	33.64283523730591	24.50662724391596	29.787349598476677	12.063187920301448
3	27.19210299447075	23.14880893543728	36.66902377639393	12.990064293698044
4	23.95120384581823	26.111417064677862	36.12580231344346	13.811576776060456
5	22.046760567256015	30.85652153605346	34.20575359260092	12.890964304089604
6	20.8692195142616	38.5413147748055	31.02789174095545	9.561573969977447
7	83.199347395611	3.798350168021009	11.574096703775757	1.428205732592238
8	84.99857161322979	2.47826009818756	11.139606431469842	1.3835618571128048
9	79.84421351286471	5.365628995273832	12.64142205769524	2.148735434166215
10	39.9355578511125	34.936964513369084	16.626023722444717	8.501453913073703
11	37.77465668932171	19.68541455984144	28.676104519531602	13.86382423130524
12	35.1770203888181	19.999551026718617	30.194937681422317	14.62849090304096
13	21.544562227515762	32.60186895990632	30.66777172025923	15.185797092318682
14	15.296374866620537	35.28756195559727	33.459516549915506	15.956546627866686
15	14.857901766818484	23.051446906918063	47.01905276051584	15.07159856574761
16	17.73744983900325	19.878509278358727	44.2400529788472	18.14398790379082
17	16.92509785988196	20.727430855399103	33.033752290578406	29.313718994140537
18	20.39508200460777	23.413015228521967	37.14508028475044	19.046822482119822
19	25.686087295613056	23.139829469809627	31.54337824152373	19.630704993053584
20	27.042312473093283	22.38866820403808	31.843067906846734	18.725951416021903
21	21.96134702122521	24.46067989761961	33.72499010629442	19.852982974860755
22	21.455093438943436	22.59537695108762	31.734228093634105	24.21530151633484
23	19.036105055402942	26.060979985567684	31.63208667211952	23.270828286909857
24	18.87719023830705	22.30901165411533	40.80663843204047	18.00715967553715
25	17.355134606896737	24.832748400804242	37.12389888397553	20.688218108323493
26	17.692805963523817	29.586977167898265	32.828745297095395	19.891471571482523
27	17.37950226966856	29.452212768438045	34.40779156922317	18.76049339267022
28	15.478607328330177	26.334491611984255	40.47273265777338	17.714168401912193
29	16.897073237318224	22.897601142999076	39.75524438810554	20.450081231577162
30	18.945043135832158	26.648519456293357	35.39922595557989	19.007211452294598
31	24.63585189240428	25.75911786888315	30.702567149566395	18.902463089146178
32	25.355512613433646	25.186025199711644	32.14963700148125	17.308825185373465
33	24.73067939433504	26.01818269374555	29.99481870350275	19.256319208416656
34	18.94203791144871	26.12133792589551	33.2623665888567	21.67425757379908
35	19.9345223159635	25.311085983090365	33.3330436731518	21.421348027794345
36	25.725770740483668	24.92862592089308	30.79355665409179	18.552046684531458
37	20.11259091256354	29.715839741159662	31.39119802364858	18.780371322628216
38	20.48900431847123	29.815338013517717	28.59282721733964	21.102830450671416
39	21.16213837284117	29.430922745095057	29.82109500962577	19.585843872437998
40	22.26979890703972	25.704516924663366	29.88463921195051	22.141044956346402
41	18.04926902442813	25.81201705953639	30.467652742339663	25.671061173695815
42	21.507702969415142	25.53502951094137	28.720712187488346	24.23655533215514
43	21.71267375537546	25.993127088042574	29.106068851501	23.188130305080964
44	19.68476282443298	28.810470636242208	30.297151517982286	21.207615021342523
45	18.42213409311199	32.68210483019215	27.452471290150072	21.44328978654579
46	20.661388334008603	30.826288254605505	29.241086703620063	19.271236707765826
47	21.213118564791735	26.616982704028487	30.986035844723315	21.183862886456467
48	22.161429791622087	24.288911409968	32.37658575371571	21.173073044694206
49	20.25673306040103	24.24473823228357	33.73328162899093	21.765247078324474
50	19.24060514356826	29.99677390972813	30.346574786457083	20.41604616024653
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	981.0
1	2636.0
2	4291.0
3	35376.5
4	66462.0
5	47799.5
6	29137.0
7	28435.5
8	27734.0
9	27886.5
10	28039.0
11	27123.5
12	26208.0
13	24602.5
14	22997.0
15	21037.5
16	19078.0
17	17790.0
18	16502.0
19	16057.5
20	15613.0
21	15391.5
22	15170.0
23	16205.5
24	17241.0
25	18748.5
26	20256.0
27	25471.0
28	30686.0
29	38303.0
30	45920.0
31	51498.0
32	57076.0
33	63339.0
34	69602.0
35	80033.0
36	90464.0
37	92395.0
38	94326.0
39	110589.5
40	126853.0
41	165283.5
42	203714.0
43	233123.5
44	262533.0
45	259844.0
46	257155.0
47	241112.5
48	225070.0
49	209351.0
50	193632.0
51	180653.0
52	167674.0
53	151357.0
54	135040.0
55	120805.0
56	106570.0
57	100071.5
58	93573.0
59	91508.0
60	89443.0
61	80110.5
62	70778.0
63	58600.5
64	46423.0
65	38458.0
66	30493.0
67	25987.0
68	21481.0
69	16954.5
70	12428.0
71	10911.5
72	9395.0
73	7290.5
74	5186.0
75	4064.0
76	2942.0
77	2360.0
78	1778.0
79	1464.5
80	1151.0
81	749.0
82	347.0
83	290.5
84	234.0
85	167.5
86	101.0
87	75.0
88	49.0
89	30.5
90	12.0
91	11.5
92	11.0
93	6.0
94	1.0
95	3.5
96	6.0
97	3.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2761857.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.538344723125164
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.78608270077181	13.99315822513909
2	8.528007606439111	2.991342744063253
3	2.8899355639884545	1.5205405844654596
4	1.4258040256649502	1.0002497003892596
5	0.827659711907198	0.725789067043545
6	0.53391922859583	0.5618435691251238
7	0.4079020997501797	0.5007749347093667
8	0.31719853905649664	0.4450509858915615
9	0.25836394456555895	0.4078148331435455
>10	2.606111519491387	10.579237594338696
>50	0.9485537465166493	12.112654908608034
>100	1.456211439080783	51.85742719245858
>500	0.01215430444056243	1.3792967445031221
>1k	0.0016764557849051629	0.357751992085944
>5k	2.0955697311314536E-4	0.23808479604815544
>10k+	2.0955697311314536E-4	1.3289821279872338
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36160	1.3092640205484931	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	6478	0.23455233199981027	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.6207522692159656E-5	0.0	0.0	0.043376612185207275	0.0
2	3.6207522692159656E-5	0.0	0.0	0.1753892399208214	0.0
3	3.6207522692159656E-5	0.0	0.0	0.2489629260312898	0.0
4	3.6207522692159656E-5	0.0	0.0	0.3565716834723883	0.0
5	3.6207522692159656E-5	0.0	0.0	0.5848239065237628	0.0
6	3.6207522692159656E-5	0.0	0.0	0.77875139806297	0.0
7	3.6207522692159656E-5	0.0	0.0	0.9107640257985841	0.0
8	3.6207522692159656E-5	0.0	0.0	1.2609993927998444	0.0
9	3.6207522692159656E-5	0.0	0.0	1.4230642643699511	0.0
10	3.6207522692159656E-5	0.0	0.0	1.742776689741721	0.0
11	3.6207522692159656E-5	0.0	0.0	2.0361300385935985	0.0
12	3.6207522692159656E-5	0.0	0.0	2.3159779814813004	0.0
13	3.6207522692159656E-5	0.0	0.0	2.4246005495577796	0.0
14	3.6207522692159656E-5	0.0	0.0	2.4617856753626275	0.0
15	3.6207522692159656E-5	0.0	0.0	2.527683366662358	0.0
16	3.6207522692159656E-5	0.0	0.0	2.6696168556156237	0.0
17	3.6207522692159656E-5	0.0	0.0	2.862892611746372	0.0
18	3.6207522692159656E-5	0.0	0.0	3.1402060280456228	0.0
19	3.6207522692159656E-5	0.0	0.0	3.26019775824744	0.0
20	3.6207522692159656E-5	0.0	0.0	3.4047743963572334	0.0
21	3.6207522692159656E-5	0.0	0.0	3.5695910396519444	0.0
22	3.6207522692159656E-5	0.0	0.0	3.7553718385854156	0.0
23	3.6207522692159656E-5	0.0	0.0	3.94690963362694	0.0
24	3.6207522692159656E-5	0.0	0.0	4.088734500012129	0.0
25	3.6207522692159656E-5	0.0	0.0	4.207857249669335	0.0
26	3.6207522692159656E-5	0.0	0.0	4.3238299448523225	0.0
27	3.6207522692159656E-5	0.0	0.0	4.439006074536082	0.0
28	3.6207522692159656E-5	0.0	0.0	4.563270292415574	0.0
29	3.6207522692159656E-5	0.0	0.0	4.699048502511173	0.0
30	3.6207522692159656E-5	0.0	0.0	4.874256704818533	0.0
31	3.6207522692159656E-5	0.0	0.0	5.028138676260212	0.0
32	3.6207522692159656E-5	0.0	0.0	5.178001612683061	0.0
33	3.6207522692159656E-5	0.0	0.0	5.31982647906825	0.0
34	3.6207522692159656E-5	0.0	0.0	5.475373996553768	0.0
35	3.6207522692159656E-5	0.0	0.0	5.656158157355721	0.0
36	3.6207522692159656E-5	0.0	0.0	5.822567931648887	0.0
37	3.6207522692159656E-5	3.6207522692159656E-5	0.0	5.982279314244003	0.0
38	3.6207522692159656E-5	3.6207522692159656E-5	0.0	6.145321788926799	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGCT	30	2.5290137E-6	44.0	44
GTCGATA	35	1.4470788E-7	44.0	27
CTATCGA	20	7.858404E-4	44.0	31
TAAGTCG	50	2.7284841E-11	44.0	1
TTAACGC	25	4.4444685E-5	44.0	13
CTACCGA	35	1.4470788E-7	44.0	12
ACGTAAC	20	7.858404E-4	44.0	16
GTACGTC	30	2.5290137E-6	44.0	25
CCGGATA	95	0.0	43.999996	20
CGTTTTT	24035	0.0	42.846684	1
CGGTCTA	340	0.0	42.058826	31
GCTACGA	200	0.0	41.8	10
TAATGCG	170	0.0	41.411766	1
AAACGCG	75	0.0	41.066666	1
CGACGGT	360	0.0	40.944447	28
ACGGTCT	355	0.0	40.90141	30
ATAGCGG	470	0.0	40.723404	2
CGCGTCA	65	0.0	40.615387	39
ACGGGTA	225	0.0	40.08889	5
AATACGG	195	0.0	39.48718	2
>>END_MODULE