FastQCFastQC Report
Thu 26 May 2016
SRR1545890_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545890_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1178510
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138731.1771643855376706No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA33370.2831541522770278No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTTA207.856561E-444.00000425
AAGTACG408.312782E-944.0000041
AGTCACG207.856561E-444.0000041
CTATCGC207.856561E-444.00000424
GACACGC207.856561E-444.00000412
CGTTGGA207.856561E-444.00000437
TCTACGC207.856561E-444.00000432
CTACGCT207.856561E-444.00000433
ACTCGCT207.856561E-444.00000410
CTCGATA207.856561E-444.00000439
CGTAAGT207.856561E-444.00000441
ACCGTTG207.856561E-444.0000041
CGCAATA207.856561E-444.00000423
CGCGTGA207.856561E-444.00000414
ATACGCC207.856561E-444.00000423
CGGTAAT207.856561E-444.00000425
GCGCTAT207.856561E-444.00000438
CCCGCCG207.856561E-444.00000444
ACGCGAG600.044.01
TCACGAC1200.044.025
Produced by FastQC (version 0.11.5)