##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545890_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1178510
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.50177427429551	31.0	31.0	33.0	30.0	34.0
2	31.883135484637382	31.0	31.0	34.0	30.0	34.0
3	31.840770973517408	31.0	31.0	34.0	30.0	34.0
4	35.63105701266854	37.0	35.0	37.0	33.0	37.0
5	35.584114687189754	37.0	35.0	37.0	33.0	37.0
6	35.639463390213066	37.0	35.0	37.0	33.0	37.0
7	36.016443644941496	37.0	35.0	37.0	35.0	37.0
8	36.02535235169833	37.0	35.0	37.0	35.0	37.0
9	37.824814384264876	39.0	38.0	39.0	35.0	39.0
10	37.3135993754826	39.0	37.0	39.0	34.0	39.0
11	36.98358181093075	39.0	37.0	39.0	33.0	39.0
12	36.18661105972796	38.0	35.0	39.0	32.0	39.0
13	35.81222136426505	38.0	35.0	39.0	31.0	39.0
14	36.80403560427998	39.0	35.0	41.0	31.0	41.0
15	37.028222925558545	39.0	35.0	41.0	32.0	41.0
16	37.21909105565502	39.0	35.0	41.0	32.0	41.0
17	37.165956164987996	38.0	35.0	41.0	32.0	41.0
18	36.97335279293345	38.0	35.0	40.0	32.0	41.0
19	36.77256875206829	37.0	35.0	40.0	32.0	41.0
20	36.49149349602464	37.0	35.0	40.0	32.0	41.0
21	36.34322152548557	36.0	35.0	40.0	32.0	41.0
22	36.23733188517704	36.0	35.0	40.0	31.0	41.0
23	36.18714902716142	36.0	35.0	40.0	31.0	41.0
24	36.18995680987009	36.0	35.0	40.0	31.0	41.0
25	36.18152497645332	36.0	35.0	40.0	31.0	41.0
26	35.93056825992143	36.0	35.0	40.0	31.0	41.0
27	35.91212463195051	36.0	35.0	40.0	31.0	41.0
28	35.91964429661182	36.0	35.0	40.0	31.0	41.0
29	35.9044836276315	36.0	35.0	40.0	31.0	41.0
30	35.75663677015893	36.0	35.0	40.0	30.0	41.0
31	35.47508209518799	36.0	35.0	40.0	30.0	41.0
32	35.097809946457815	36.0	34.0	40.0	27.0	41.0
33	34.65688029800341	36.0	34.0	40.0	24.0	41.0
34	34.2447522719366	36.0	34.0	40.0	23.0	41.0
35	33.88083427378639	36.0	33.0	40.0	20.0	41.0
36	33.63789700554089	36.0	33.0	40.0	18.0	41.0
37	33.51407709735174	36.0	33.0	40.0	18.0	41.0
38	33.45955061900196	35.0	33.0	40.0	18.0	41.0
39	33.35638390849463	36.0	33.0	40.0	18.0	41.0
40	33.209117444909246	36.0	33.0	40.0	17.0	41.0
41	33.19708105998252	36.0	33.0	40.0	15.0	41.0
42	33.15801647843463	35.0	33.0	40.0	15.0	41.0
43	33.05193931320057	35.0	33.0	40.0	15.0	41.0
44	32.9343577907697	35.0	32.0	40.0	15.0	41.0
45	32.82032905957523	35.0	32.0	40.0	15.0	41.0
46	32.79698178208076	35.0	32.0	40.0	15.0	41.0
47	32.729178369296825	35.0	32.0	40.0	14.0	41.0
48	32.637902096715344	35.0	32.0	40.0	13.0	41.0
49	32.61257520088926	35.0	32.0	40.0	12.0	41.0
50	32.41397527386276	35.0	32.0	40.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	5.0
14	7.0
15	25.0
16	97.0
17	244.0
18	553.0
19	1255.0
20	2191.0
21	3611.0
22	5541.0
23	8520.0
24	12750.0
25	20547.0
26	30788.0
27	37035.0
28	35587.0
29	32592.0
30	33237.0
31	36491.0
32	43391.0
33	56511.0
34	100888.0
35	177153.0
36	67497.0
37	84659.0
38	134832.0
39	252503.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.173108416559895	20.906823022290858	29.671279836403595	20.248788724745655
2	34.57484450704704	23.25317562006262	30.14187406131471	12.030105811575634
3	25.813612103418727	23.051310553156103	39.117105497619875	12.017971845805295
4	22.903751347039904	26.18934077776175	37.81520733807944	13.091700537118905
5	21.483313675743098	30.433768063062683	35.88531281024344	12.197605450950777
6	19.421303170953152	37.77829632332352	32.8465604873951	9.953840018328227
7	81.57851863794113	3.18962079235645	13.715284554225251	1.5165760154771704
8	83.3907221830956	2.1087644568141126	13.006253659281636	1.4942597008086482
9	79.45049257112795	4.615913314269713	14.320710049129834	1.6128840654725034
10	43.09305818363866	32.68118217070708	17.77583558900646	6.449924056647801
11	40.49681377332394	18.11736854163308	29.913025769828007	11.472791915214975
12	37.697940619935345	19.394489652187932	30.677380760451754	12.230188967424969
13	19.405265971438514	38.182111310044036	30.404408957072913	12.008213761444535
14	13.679222068544178	38.925677338334	34.00658458562083	13.388516007500998
15	11.704694911371138	21.98012744906704	53.11554420412216	13.199633435439665
16	14.507980415948952	18.555888367514914	50.755360582430356	16.18077063410578
17	15.430331520309542	17.63438579222917	34.96177376517806	31.973508922283223
18	19.42020008315585	21.57266378732467	40.61492902054289	18.39220710897659
19	24.670049469245065	22.654029240311917	33.280583109180235	19.39533818126278
20	27.337824880569535	21.454548540105726	32.60634190630542	18.60128467301932
21	20.215356679196613	25.440429016300243	35.11128458816641	19.23292971633673
22	20.89171920475855	22.177325606061892	33.16204359742387	23.768911591755693
23	16.094475227193662	27.332139735768045	33.1820688835903	23.391316153447995
24	17.002571043096793	22.468710490364952	44.72308253642311	15.805635930115145
25	14.326480046838805	24.424145743353893	41.530237333582235	19.719136876225065
26	15.335975087186362	31.66141992855385	34.9268143672943	18.075790616965488
27	15.88039134160932	30.655573563228145	36.51016962096206	16.953865474200473
28	13.352623227634894	26.667911175976442	43.94014475906017	16.039320837328493
29	14.140991591076869	21.713010496304655	43.384443067941724	20.761554844676752
30	17.290392105285488	27.16701597780248	37.85262747028027	17.689964446631766
31	25.418536966169146	24.833051904523508	32.13676591628412	17.611645213023223
32	26.339530424009975	24.329110486970837	32.65292615251462	16.67843293650457
33	25.054517993059033	26.73842394209637	30.305979584390457	17.90107848045413
34	19.11439020458036	26.248992371723617	32.76102875665035	21.875588667045676
35	19.073066838635224	26.381108348677568	33.26760061433505	21.278224198352156
36	27.263578586520264	24.62108934162629	30.772585722649787	17.342746349203654
37	19.813323603533277	30.476788487157513	33.17256535795199	16.53732255135722
38	20.015528082069732	30.822224673528435	28.853637219879342	20.30861002452249
39	19.661182340412893	30.114636278012064	31.652255814545487	18.571925567029552
40	21.894850277044743	26.546147253735647	29.591433250460327	21.96756921875928
41	17.147414956173474	25.115442380633173	30.71607368626486	27.021068976928493
42	21.487386615302373	25.724516550559606	27.73306972363408	25.055027110503943
43	21.422389288168958	26.660783531747718	28.895554556176865	23.02127262390646
44	19.837846093796404	29.970301482380297	29.721597610542126	20.470254813281176
45	16.47045846025914	36.09897243129036	26.088790082392173	21.341779026058326
46	20.85319598476042	30.800417476304826	29.16767783048086	19.178708708453897
47	20.932872864888715	27.3671839865593	29.859059320667626	21.840883827884365
48	21.994128178802047	23.506885813442395	32.44563049952907	22.05335550822649
49	20.396941901214245	23.466410976572114	34.209892151954584	21.926754970259054
50	18.86568633274219	30.785568217494973	29.625119854731825	20.723625595031013
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	322.0
1	707.0
2	1092.0
3	12744.5
4	24397.0
5	18616.0
6	12835.0
7	13239.0
8	13643.0
9	14504.0
10	15365.0
11	15317.0
12	15269.0
13	14488.5
14	13708.0
15	12662.5
16	11617.0
17	10578.0
18	9539.0
19	9030.5
20	8522.0
21	8116.0
22	7710.0
23	7460.0
24	7210.0
25	7749.5
26	8289.0
27	10210.0
28	12131.0
29	13116.5
30	14102.0
31	16274.0
32	18446.0
33	23805.0
34	29164.0
35	31929.0
36	34694.0
37	39036.5
38	43379.0
39	52100.5
40	60822.0
41	78475.0
42	96128.0
43	110531.0
44	124934.0
45	125748.5
46	126563.0
47	117042.0
48	107521.0
49	100039.5
50	92558.0
51	84234.5
52	75911.0
53	67919.5
54	59928.0
55	51432.5
56	42937.0
57	35921.5
58	28906.0
59	25032.5
60	21159.0
61	18545.0
62	15931.0
63	12468.5
64	9006.0
65	7585.0
66	6164.0
67	4720.0
68	3276.0
69	2701.0
70	2126.0
71	1673.0
72	1220.0
73	1055.0
74	890.0
75	640.0
76	390.0
77	379.5
78	369.0
79	267.5
80	166.0
81	119.5
82	73.0
83	69.0
84	65.0
85	36.0
86	7.0
87	15.5
88	24.0
89	12.0
90	0.0
91	1.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1178510.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.37370720037689
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.16008114340822	18.970119730048538
2	8.066842704868163	3.7710403883016954
3	2.532016921099699	1.7754786642055247
4	1.2352336224442904	1.1548795606029496
5	0.7055410599786728	0.8245555076892523
6	0.4939061792253076	0.6926651050601527
7	0.35835498128420074	0.5863259084433414
8	0.2955321076447968	0.5526144761919798
9	0.2295973546494347	0.48298872074012805
>10	2.952571887161654	16.171740577171096
>50	0.9809360151394889	16.60038186244302
>100	0.9868142807412522	36.6805982947794
>500	0.0018369588249092558	0.2587355762559911
>1k	3.6739176498185115E-4	0.2865584526937412
>5k	0.0	0.0
>10k+	3.6739176498185115E-4	1.191317175373171
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13873	1.1771643855376706	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3337	0.2831541522770278	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06830659052532435	0.0
2	0.0	0.0	0.0	0.2386063758474684	0.0
3	0.0	0.0	0.0	0.3193014908655845	0.0
4	0.0	0.0	0.0	0.4577814358809005	0.0
5	0.0	0.0	0.0	0.7503542608887493	0.0
6	0.0	0.0	0.0	1.0079676880128297	0.0
7	0.0	0.0	0.0	1.1936258495897363	0.0
8	0.0	0.0	0.0	1.7203078463483552	0.0
9	0.0	0.0	0.0	1.935409966822513	0.0
10	0.0	0.0	0.0	2.3300608395346667	0.0
11	0.0	0.0	0.0	2.655641445554132	0.0
12	0.0	0.0	0.0	2.984446462058022	0.0
13	0.0	0.0	0.0	3.1079074424485156	0.0
14	0.0	0.0	0.0	3.144818457204436	0.0
15	0.0	0.0	0.0	3.219064751253702	0.0
16	0.0	0.0	0.0	3.3798610109375398	0.0
17	0.0	0.0	0.0	3.594793425596728	0.0
18	0.0	0.0	0.0	3.904251979194067	0.0
19	0.0	0.0	0.0	4.031531340421379	0.0
20	0.0	0.0	0.0	4.19046083614055	0.0
21	0.0	0.0	0.0	4.377476644237214	0.0
22	0.0	0.0	0.0	4.575353624491943	0.0
23	0.0	0.0	0.0	4.794104419987951	0.0
24	0.0	0.0	0.0	4.95023376976012	0.0
25	0.0	0.0	0.0	5.078531365877252	0.0
26	0.0	0.0	0.0	5.218878074857235	0.0
27	0.0	0.0	0.0	5.350994051811186	0.0
28	0.0	0.0	0.0	5.489898261363925	0.0
29	0.0	0.0	0.0	5.647894375100763	0.0
30	0.0	0.0	0.0	5.864014730464739	0.0
31	0.0	0.0	0.0	6.045430246667402	0.0
32	0.0	0.0	0.0	6.212929886042545	0.0
33	0.0	0.0	0.0	6.380344672510204	0.0
34	0.0	0.0	0.0	6.557008425893713	0.0
35	0.0	0.0	0.0	6.778813926059176	0.0
36	0.0	0.0	0.0	6.966423704508235	0.0
37	0.0	0.0	0.0	7.1534395126048995	0.0
38	0.0	0.0	0.0	7.337146057309654	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTTA	20	7.856561E-4	44.000004	25
AAGTACG	40	8.312782E-9	44.000004	1
AGTCACG	20	7.856561E-4	44.000004	1
CTATCGC	20	7.856561E-4	44.000004	24
GACACGC	20	7.856561E-4	44.000004	12
CGTTGGA	20	7.856561E-4	44.000004	37
TCTACGC	20	7.856561E-4	44.000004	32
CTACGCT	20	7.856561E-4	44.000004	33
ACTCGCT	20	7.856561E-4	44.000004	10
CTCGATA	20	7.856561E-4	44.000004	39
CGTAAGT	20	7.856561E-4	44.000004	41
ACCGTTG	20	7.856561E-4	44.000004	1
CGCAATA	20	7.856561E-4	44.000004	23
CGCGTGA	20	7.856561E-4	44.000004	14
ATACGCC	20	7.856561E-4	44.000004	23
CGGTAAT	20	7.856561E-4	44.000004	25
GCGCTAT	20	7.856561E-4	44.000004	38
CCCGCCG	20	7.856561E-4	44.000004	44
ACGCGAG	60	0.0	44.0	1
TCACGAC	120	0.0	44.0	25
>>END_MODULE