FastQCFastQC Report
Thu 26 May 2016
SRR1545889_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545889_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2862968
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT410281.4330582807771517No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT288250.043.183351
TCGTTGA3800.040.52631424
CGACAGG3650.040.3835642
CATACGA9850.039.979718
ATTAGCG1450.039.4482771
TAAGGGA47450.038.853534
CGGTCTA3400.038.82353231
CGCGTAA850.038.82353231
GCGTAAC850.038.82353232
ACGTCGA404.1274143E-738.524
GTTTTTT340850.037.894092
CTACGGG6300.037.7142873
ACATACG10150.037.71428317
ACGGGAT16000.037.6755
ATACGAA10150.037.49753619
CGTTGAT4050.037.48148325
TATAACG1350.037.481481
GGGCGAT41200.037.378647
ATAGGGA48350.037.1747674
TGATGCG1600.037.1251
Produced by FastQC (version 0.11.5)