##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545889_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2862968
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.564360132561735	31.0	31.0	33.0	30.0	34.0
2	31.97646742820737	31.0	31.0	34.0	30.0	34.0
3	31.967567223943824	33.0	31.0	34.0	30.0	34.0
4	35.76737497589914	37.0	35.0	37.0	35.0	37.0
5	35.723872917894994	37.0	35.0	37.0	33.0	37.0
6	35.77962799444492	37.0	35.0	37.0	33.0	37.0
7	36.04378672761973	37.0	35.0	37.0	35.0	37.0
8	36.0344439756225	37.0	35.0	37.0	35.0	37.0
9	37.83632405252172	39.0	38.0	39.0	35.0	39.0
10	37.37289274626891	39.0	37.0	39.0	34.0	39.0
11	37.13766482894675	39.0	37.0	39.0	33.0	39.0
12	36.763490894763756	39.0	35.0	39.0	33.0	39.0
13	36.61044901654507	39.0	35.0	39.0	33.0	39.0
14	37.718052035510006	40.0	36.0	41.0	33.0	41.0
15	37.81197798927546	40.0	36.0	41.0	33.0	41.0
16	37.8905279416326	40.0	36.0	41.0	33.0	41.0
17	37.80175258682598	40.0	36.0	41.0	33.0	41.0
18	37.62329652304881	39.0	36.0	41.0	33.0	41.0
19	37.451065467724405	39.0	36.0	41.0	33.0	41.0
20	37.22415374534399	39.0	35.0	41.0	33.0	41.0
21	37.12389136029463	39.0	35.0	41.0	32.0	41.0
22	37.03815131709471	38.0	35.0	41.0	32.0	41.0
23	36.9401407909554	38.0	35.0	41.0	32.0	41.0
24	36.99789623914763	38.0	35.0	41.0	32.0	41.0
25	36.96970591358339	38.0	35.0	41.0	32.0	41.0
26	36.738302698458384	38.0	35.0	41.0	32.0	41.0
27	36.71689274906321	38.0	35.0	41.0	31.0	41.0
28	36.68569680136138	38.0	35.0	41.0	31.0	41.0
29	36.60403120118702	38.0	35.0	41.0	31.0	41.0
30	36.45657897678214	38.0	35.0	41.0	31.0	41.0
31	36.25424559408278	38.0	35.0	41.0	30.0	41.0
32	35.96686375816984	38.0	35.0	41.0	30.0	41.0
33	35.58591713215097	38.0	35.0	41.0	27.0	41.0
34	35.17791711259085	38.0	34.0	41.0	24.0	41.0
35	34.83207252054511	38.0	34.0	41.0	23.0	41.0
36	34.63233714103686	38.0	34.0	40.0	21.0	41.0
37	34.54070076927161	38.0	34.0	40.0	21.0	41.0
38	34.42347382157258	38.0	34.0	40.0	20.0	41.0
39	34.29474447496444	38.0	34.0	40.0	19.0	41.0
40	34.17452622593057	38.0	33.0	40.0	18.0	41.0
41	34.12170551679236	38.0	34.0	40.0	18.0	41.0
42	34.09155219338812	38.0	33.0	40.0	18.0	41.0
43	34.003679747730324	38.0	33.0	40.0	18.0	41.0
44	33.8736147243001	37.0	33.0	40.0	18.0	41.0
45	33.79044159767067	37.0	33.0	40.0	18.0	41.0
46	33.743194475104154	37.0	33.0	40.0	18.0	41.0
47	33.684531926308644	37.0	33.0	40.0	18.0	41.0
48	33.553207021524514	37.0	33.0	40.0	18.0	41.0
49	33.478393401532955	36.0	33.0	40.0	18.0	41.0
50	33.35027670585211	36.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	1.0
11	1.0
12	4.0
13	16.0
14	19.0
15	55.0
16	155.0
17	435.0
18	1090.0
19	2214.0
20	4147.0
21	6909.0
22	10410.0
23	16499.0
24	25214.0
25	40513.0
26	62398.0
27	75566.0
28	73926.0
29	69628.0
30	70026.0
31	77633.0
32	93623.0
33	118789.0
34	193168.0
35	282525.0
36	201022.0
37	261645.0
38	413187.0
39	762148.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.996691545277486	22.738151456809856	28.94422850692009	19.320928490992564
2	36.11297786073753	23.070184507825445	28.860923349475094	11.955914281961936
3	26.3539096490076	23.36798734739613	37.832417267674664	12.445685735921604
4	24.06949710929357	25.694978078693158	37.1281830603765	13.107341751636763
5	21.492136831428084	30.65654244127074	34.95795272598227	12.893368001318912
6	19.655371628324172	38.767949903736266	31.88558167607881	9.691096791860755
7	82.70333444174018	3.028081347748211	12.875973465298948	1.3926107452126604
8	83.83307113457083	2.2626868340826722	12.541495399180153	1.3627466321663393
9	78.70514095861358	5.358495100189733	13.670254085969525	2.266109855227163
10	34.234717258453465	38.25096892455662	18.514807011465027	8.999506805524895
11	31.867453635527887	20.862789943862452	32.38778777827765	14.881968642332014
12	29.811265791304685	20.42663417823741	33.495170047307546	16.26692998315035
13	22.541642100086342	26.37787079701904	33.91476956780516	17.16571753508946
14	17.26620765583129	28.204925797284496	35.81447644542307	18.714390101461138
15	17.04849652528425	23.329321179978262	41.956005096808624	17.66617719792886
16	20.561668869508846	21.884072752472257	39.3568492557374	18.197409122281492
17	20.291809059689108	21.55570722411148	34.95714936387693	23.195334352322487
18	21.64547420718639	22.57772353725225	36.37061259504123	19.40618966052013
19	23.33515428743877	24.073653634969023	33.23278499794618	19.358407079646017
20	25.06402446691685	23.222299376032147	33.30756753131715	18.406108625733854
21	22.780834434754425	24.03348552970204	34.52200653308036	18.663673502463176
22	22.381877827485322	21.540198842599708	34.5737011381196	21.504222191795368
23	19.508042003962323	24.54571619382403	34.54802847953592	21.398213322677726
24	19.69955654411785	23.222578806329654	38.518383719273146	18.559480930279346
25	19.447754917274658	24.793466081353337	35.17293941112859	20.585839590243413
26	19.55117905614034	26.87274185390825	33.22635810110347	20.349720988847935
27	18.50251207837461	26.29694079710287	35.051107801414474	20.149439323108048
28	17.980955428073244	24.78117114826292	37.680931117637364	19.556942306026475
29	18.971954978190464	22.255680119372624	37.51264422096231	21.259720681474608
30	20.556359693856166	24.810546258288603	35.81328886665865	18.81980518119658
31	23.489958672258997	24.266460540250538	32.79694359140584	19.446637196084623
32	24.54739277560909	23.96935627642363	32.45830899961159	19.024941948355693
33	24.184168317634008	24.76744413489777	31.753341287782472	19.29504625968575
34	20.581822779716713	25.381562071249135	33.55164989619164	20.484965252842503
35	20.87553895118632	25.7942107630962	32.64255835203188	20.6876919336856
36	23.38726803792428	26.39166766795857	30.633000438705565	19.588063855411587
37	21.56915480717912	28.79483808411411	30.459229722441883	19.176777386264884
38	21.0462359341774	28.655856439890353	29.645214337009705	20.652693288922546
39	21.193216270667364	28.271115849007046	29.463654501202946	21.072013379122644
40	22.472029027219307	26.83529819404199	29.45478258925702	21.237890189481686
41	20.08810437280473	26.415733602331564	30.122271712432692	23.373890312431016
42	21.11815430699889	25.907973822969733	29.2789860033364	23.69488586669498
43	21.44016279609133	26.208675751877074	29.517619477409458	22.83354197462214
44	20.921505235126624	27.441138007829636	29.56274747045723	22.074609286586508
45	19.57447655719519	29.406685649298208	28.632733582771447	22.38610421073515
46	21.09150364237393	28.37785822265565	29.692263413352855	20.838374721617566
47	20.541549888088166	26.668163947344155	31.23856082219571	21.55172534237197
48	21.04382584786138	25.844333572711953	31.99305755425838	21.118783025168288
49	21.404779934669197	25.28491411709806	31.753376216569656	21.556929731663086
50	20.122998231206214	27.739953782228792	31.165419941822613	20.97162804474238
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	735.0
1	2776.0
2	4817.0
3	40902.5
4	76988.0
5	54462.0
6	31936.0
7	31088.0
8	30240.0
9	30009.5
10	29779.0
11	28923.5
12	28068.0
13	26870.5
14	25673.0
15	23876.0
16	22079.0
17	20702.5
18	19326.0
19	19054.5
20	18783.0
21	17841.5
22	16900.0
23	18335.0
24	19770.0
25	21430.5
26	23091.0
27	27517.5
28	31944.0
29	38283.5
30	44623.0
31	52029.0
32	59435.0
33	69542.5
34	79650.0
35	89595.5
36	99541.0
37	102773.5
38	106006.0
39	123365.5
40	140725.0
41	160878.0
42	181031.0
43	200091.0
44	219151.0
45	221062.0
46	222973.0
47	215609.0
48	208245.0
49	200971.5
50	193698.0
51	181200.5
52	168703.0
53	157832.0
54	146961.0
55	140358.5
56	133756.0
57	124285.5
58	114815.0
59	108824.5
60	102834.0
61	93360.0
62	83886.0
63	73812.5
64	63739.0
65	53744.5
66	43750.0
67	35256.0
68	26762.0
69	21858.0
70	16954.0
71	13946.5
72	10939.0
73	8645.0
74	6351.0
75	5217.0
76	4083.0
77	3280.0
78	2477.0
79	1680.5
80	884.0
81	656.5
82	429.0
83	330.0
84	231.0
85	179.5
86	128.0
87	86.0
88	44.0
89	35.0
90	26.0
91	15.5
92	5.0
93	4.0
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2862968.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.036884519077358
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.26486852548096	16.464490010713153
2	7.34542992360946	3.0904992209189484
3	2.6989636674255992	1.7033336097845353
4	1.488722977434798	1.252723734287714
5	1.0149583929366546	1.067578125193837
6	0.7411571964378418	0.9354983011167606
7	0.5661327171299176	0.8336768014913494
8	0.4822617322875721	0.8116227496083083
9	0.3854315499978175	0.7297451106581769
>10	4.801024402125983	23.41459525200125
>50	1.3141495741442222	19.761035339433526
>100	0.8935180548712394	27.82180704583525
>500	0.0025359645884316124	0.38884157792902774
>1k	6.762572235817633E-4	0.26535690230755293
>5k	0.0	0.0
>10k+	1.6906430589544083E-4	1.4591962187205845
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41028	1.4330582807771517	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.034544570529604246	0.0
2	0.0	0.0	0.0	0.12654699598458663	0.0
3	0.0	0.0	0.0	0.1787655328316628	0.0
4	0.0	0.0	0.0	0.2501949026325128	0.0
5	0.0	0.0	0.0	0.45547138494038353	0.0
6	0.0	0.0	0.0	0.6514917386432542	0.0
7	0.0	0.0	0.0	0.7979132145381995	0.0
8	0.0	0.0	0.0	1.173223032880563	0.0
9	0.0	0.0	0.0	1.3495784793962071	0.0
10	0.0	0.0	0.0	1.6856632697256833	0.0
11	0.0	0.0	0.0	1.9168569121275543	0.0
12	0.0	0.0	0.0	2.1551410983287274	0.0
13	0.0	0.0	0.0	2.241450131471955	0.0
14	0.0	0.0	0.0	2.2840283230549554	0.0
15	0.0	0.0	0.0	2.3342908478194655	0.0
16	0.0	0.0	0.0	2.434291965540656	0.0
17	0.0	0.0	0.0	2.5592671661017516	0.0
18	0.0	0.0	0.0	2.7199395871696783	0.0
19	0.0	0.0	0.0	2.8202899927627554	0.0
20	0.0	0.0	0.0	2.927416583070436	0.0
21	0.0	0.0	0.0	3.054347795714098	0.0
22	0.0	0.0	0.0	3.2068468805798735	0.0
23	0.0	0.0	0.0	3.357948813958102	0.0
24	0.0	0.0	0.0	3.4745760343811036	0.0
25	0.0	0.0	0.0	3.5784542474802374	0.0
26	0.0	0.0	0.0	3.679677872753031	0.0
27	0.0	0.0	0.0	3.792113638713391	0.0
28	0.0	0.0	0.0	3.907727924307921	0.0
29	0.0	0.0	0.0	4.03818694445764	0.0
30	0.0	0.0	0.0	4.199348368546208	0.0
31	0.0	0.0	0.0	4.333439982563549	0.0
32	0.0	0.0	0.0	4.462955925459174	0.0
33	0.0	0.0	0.0	4.592402010780421	0.0
34	0.0	0.0	0.0	4.735889468551517	0.0
35	0.0	0.0	0.0	4.900508842571765	0.0
36	0.0	0.0	0.0	5.048571971464578	0.0
37	0.0	0.0	0.0	5.194888660997957	0.0
38	0.0	0.0	0.0	5.365166498542771	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	28825	0.0	43.18335	1
TCGTTGA	380	0.0	40.526314	24
CGACAGG	365	0.0	40.383564	2
CATACGA	985	0.0	39.9797	18
ATTAGCG	145	0.0	39.448277	1
TAAGGGA	4745	0.0	38.85353	4
CGGTCTA	340	0.0	38.823532	31
CGCGTAA	85	0.0	38.823532	31
GCGTAAC	85	0.0	38.823532	32
ACGTCGA	40	4.1274143E-7	38.5	24
GTTTTTT	34085	0.0	37.89409	2
CTACGGG	630	0.0	37.714287	3
ACATACG	1015	0.0	37.714283	17
ACGGGAT	1600	0.0	37.675	5
ATACGAA	1015	0.0	37.497536	19
CGTTGAT	405	0.0	37.481483	25
TATAACG	135	0.0	37.48148	1
GGGCGAT	4120	0.0	37.37864	7
ATAGGGA	4835	0.0	37.174767	4
TGATGCG	160	0.0	37.125	1
>>END_MODULE