FastQCFastQC Report
Thu 26 May 2016
SRR1545888_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545888_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2629739
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350701.3335924211490189No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA71410.27154786083333743No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATTA207.858333E-444.00000442
GCGCATT207.858333E-444.00000441
CGTAGTC302.5289646E-644.00000413
CGCTATA207.858333E-444.00000416
CGGATAT207.858333E-444.00000432
CGCTAGT207.858333E-444.00000418
CTACCGT207.858333E-444.0000049
CGTACCC207.858333E-444.00000432
ATTACGC502.7284841E-1144.04
TATCGTG700.044.01
TACGTCG551.8189894E-1244.01
TAGTACG1050.044.01
CGTTTTT237050.043.1647381
TACGACG1450.040.965521
TGCGTAG2550.040.549021
TATCACG557.8216544E-1140.01
CACGAAC557.8216544E-1140.031
TAATGCG2050.039.7073171
ACTTACG501.3496901E-939.6000021
ACGACTA950.039.3684210
Produced by FastQC (version 0.11.5)