##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545888_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2629739
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.499186801427822	31.0	31.0	33.0	30.0	34.0
2	31.89418493622371	31.0	31.0	34.0	30.0	34.0
3	31.910528002969116	31.0	31.0	34.0	30.0	34.0
4	35.690285613895526	37.0	35.0	37.0	33.0	37.0
5	35.62269259420802	37.0	35.0	37.0	33.0	37.0
6	35.6682031943094	37.0	35.0	37.0	33.0	37.0
7	35.99735487057841	37.0	35.0	37.0	35.0	37.0
8	35.992436511760296	37.0	35.0	37.0	35.0	37.0
9	37.77872024562134	39.0	38.0	39.0	35.0	39.0
10	37.29477450043522	39.0	37.0	39.0	34.0	39.0
11	37.030417087018904	39.0	37.0	39.0	33.0	39.0
12	36.28421337630845	38.0	35.0	39.0	32.0	39.0
13	35.93559398860495	38.0	35.0	39.0	31.0	39.0
14	36.86339442811625	39.0	35.0	41.0	31.0	41.0
15	37.108367408324554	39.0	35.0	41.0	32.0	41.0
16	37.27975133653948	39.0	35.0	41.0	32.0	41.0
17	37.21892819021203	39.0	35.0	41.0	32.0	41.0
18	37.06812729324089	39.0	35.0	40.0	32.0	41.0
19	36.90713489057279	38.0	35.0	40.0	32.0	41.0
20	36.65145742600311	38.0	35.0	40.0	32.0	41.0
21	36.515902148464164	38.0	35.0	40.0	31.0	41.0
22	36.439197197896824	38.0	35.0	40.0	31.0	41.0
23	36.37286437931673	37.0	35.0	40.0	31.0	41.0
24	36.384374647065734	37.0	35.0	40.0	31.0	41.0
25	36.3759757147002	37.0	35.0	40.0	31.0	41.0
26	36.14652366641709	37.0	35.0	40.0	31.0	41.0
27	36.1099599618061	37.0	35.0	40.0	31.0	41.0
28	36.11385730675173	37.0	35.0	40.0	31.0	41.0
29	36.08515788068702	37.0	35.0	40.0	31.0	41.0
30	35.9406317509076	37.0	35.0	40.0	30.0	41.0
31	35.69190592678589	37.0	35.0	40.0	30.0	41.0
32	35.41235575089391	37.0	34.0	40.0	29.0	41.0
33	35.068862727441775	37.0	34.0	40.0	26.0	41.0
34	34.737328305204436	37.0	34.0	40.0	24.0	41.0
35	34.448030393890804	37.0	34.0	40.0	23.0	41.0
36	34.25068267231082	37.0	33.0	40.0	22.0	41.0
37	34.13091641413844	37.0	33.0	40.0	21.0	41.0
38	34.04198439464905	36.0	33.0	40.0	21.0	41.0
39	33.94787049209066	36.0	33.0	40.0	20.0	41.0
40	33.77538873629664	36.0	33.0	40.0	18.0	41.0
41	33.725979650452004	36.0	33.0	40.0	18.0	41.0
42	33.690175717057855	36.0	33.0	40.0	18.0	41.0
43	33.591256014380136	36.0	33.0	40.0	18.0	41.0
44	33.468171175922784	35.0	33.0	40.0	18.0	41.0
45	33.39001398998152	35.0	33.0	40.0	18.0	41.0
46	33.36494001876232	35.0	33.0	40.0	18.0	41.0
47	33.32051697906142	35.0	33.0	40.0	18.0	41.0
48	33.229886311911564	35.0	33.0	40.0	18.0	41.0
49	33.16568716515213	35.0	33.0	40.0	18.0	41.0
50	32.99943074198618	35.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	0.0
10	1.0
11	1.0
12	7.0
13	10.0
14	19.0
15	82.0
16	200.0
17	585.0
18	1360.0
19	2707.0
20	4744.0
21	7672.0
22	11506.0
23	17137.0
24	25322.0
25	38721.0
26	57479.0
27	71030.0
28	71452.0
29	69229.0
30	72251.0
31	81345.0
32	97892.0
33	126325.0
34	220539.0
35	353953.0
36	164090.0
37	208107.0
38	324886.0
39	601085.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.87747491290961	21.42897070773944	28.966106522358302	18.72744785699265
2	34.66153865459652	23.81551933480851	29.22111281765985	12.301829192935116
3	26.82863204295179	22.784846709122082	37.4845184255928	12.90200282233332
4	23.97458455002569	26.24435352709908	36.01536122025798	13.765700702617256
5	22.00324823109822	30.646501420863437	34.64191693548295	12.708333412555389
6	20.427882767073083	38.249119019035724	31.13894572807415	10.184052485817034
7	83.1374140171325	3.4617503866353276	11.984231134724777	1.4166044615073967
8	84.29064633410388	2.5286920108801674	11.586282897276117	1.5943787577398365
9	79.60056112032412	5.4197393733750765	12.79564245729329	2.1840570490075253
10	40.98266025639807	33.65159051905911	17.176647568446906	8.18910165609591
11	40.67331396765991	19.401126879891883	27.731421255113148	12.19413789733506
12	37.119311079920855	19.81569273604719	28.671172310255884	14.393823873776066
13	22.128621889852948	33.55899577866853	30.189535919724353	14.12284641175417
14	15.447806797556716	35.40275289677036	34.4612906451933	14.688149660479613
15	15.13115940403211	21.982751900473772	49.124989209955814	13.761099485538297
16	18.51575384477319	19.207571549876242	45.53664831376802	16.74002629158255
17	18.502900858222052	19.336709840786483	33.35791118434187	28.8024781166496
18	21.912440740316814	21.96541177660597	37.18787301705607	18.934274466021154
19	26.402772290329953	22.307080664659114	32.54900961654369	18.741137428467237
20	27.40937408617357	23.170892624705342	31.417908773456226	18.001824515664865
21	22.59155756521845	24.319903990472056	34.14441509214413	18.944123352165366
22	21.32496799112003	21.228532565399075	33.34977349463198	24.096725948848917
23	18.580475096578024	26.717100062021366	32.02116255643621	22.6812622849644
24	18.748704719365687	23.350986542770976	40.86496796830408	17.03534076955926
25	17.835876488122963	24.187799625742326	38.21242336216636	19.763900523968346
26	17.630342783067064	30.299242624458167	32.23867463653237	19.831739955942396
27	17.053783664462518	29.109162544267704	35.13029239783872	18.70676139343106
28	14.712372596672141	25.692815903023074	40.73031582221658	18.864495678088204
29	16.37249932407741	22.329402271480173	40.53569574775292	20.762402656689506
30	19.44101677010532	26.354744710406624	35.910141652840835	18.294096866647223
31	25.92785823992419	24.127033139030146	30.985812660495966	18.959295960549696
32	26.6767918793462	24.871175428436054	31.55678187074839	16.895250821469354
33	26.169783389150027	25.193754969599645	29.922361116445394	18.714100524804934
34	18.632913760643167	26.654204086413134	32.244264544884494	22.4686176080592
35	19.529428585878676	26.36641887274745	32.85717708107154	21.246975460302334
36	27.165965900037985	24.55840674682925	29.511483839270742	18.764143513862024
37	19.64887009699442	29.95776386934217	31.299075687739354	19.094290345924065
38	20.316198679792937	30.221782465864482	27.930338333956335	21.531680520386242
39	19.942891670998527	29.10353460932815	30.43986494477209	20.513708774901236
40	23.066509642211642	26.192104995971082	28.857350482310224	21.884034879507052
41	18.448750997722588	25.06568142313743	29.862165028544656	26.623402550595326
42	21.940922654301435	24.430257147192176	28.499102002137853	25.12971819636854
43	21.563965093113804	25.43887435216955	29.460185972828484	23.536974581888163
44	19.49638348140253	28.580212713124766	30.62052926164916	21.30287454382355
45	17.730124548481808	33.019094290345926	26.880310175268345	22.37047098590392
46	20.428225006359945	30.25311637390631	28.867047262104716	20.451611357629027
47	20.47412309738723	26.52841213519669	30.52755425538428	22.469910512031802
48	22.083370250811964	24.638604819717848	31.9938594666619	21.284165462808286
49	21.146927508775587	23.12423400192947	33.59976028039284	22.1290782089021
50	18.736117918926556	29.78782305012018	30.228399092077197	21.247659938876062
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	700.0
1	2153.5
2	3607.0
3	32831.0
4	62055.0
5	44026.5
6	25998.0
7	25912.0
8	25826.0
9	26191.5
10	26557.0
11	25906.0
12	25255.0
13	23645.5
14	22036.0
15	20425.5
16	18815.0
17	17159.5
18	15504.0
19	14479.0
20	13454.0
21	14870.0
22	16286.0
23	16382.5
24	16479.0
25	18204.5
26	19930.0
27	24560.5
28	29191.0
29	32979.5
30	36768.0
31	42249.5
32	47731.0
33	57281.0
34	66831.0
35	72085.5
36	77340.0
37	84871.0
38	92402.0
39	106598.5
40	120795.0
41	153944.5
42	187094.0
43	215842.5
44	244591.0
45	240093.5
46	235596.0
47	228845.0
48	222094.0
49	211816.0
50	201538.0
51	186072.0
52	170606.0
53	151893.0
54	133180.0
55	124917.0
56	116654.0
57	106860.5
58	97067.0
59	87351.5
60	77636.0
61	68660.0
62	59684.0
63	50739.0
64	41794.0
65	34879.5
66	27965.0
67	23496.5
68	19028.0
69	15224.0
70	11420.0
71	9930.0
72	8440.0
73	6994.5
74	5549.0
75	4347.0
76	3145.0
77	2362.0
78	1579.0
79	1192.5
80	806.0
81	560.0
82	314.0
83	289.0
84	264.0
85	169.0
86	74.0
87	57.5
88	41.0
89	26.0
90	11.0
91	6.5
92	2.0
93	3.0
94	4.0
95	2.5
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2629739.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.32516365813104
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.67864922638493	14.784494507904512
2	8.199091636184436	3.004993921621863
3	2.7308149977564438	1.5012789526189672
4	1.3432199608080082	0.9845890244270045
5	0.7711739395831088	0.7065944325873063
6	0.5420353284766367	0.5959731661693909
7	0.3928789864725	0.5039700207464449
8	0.31614379817726346	0.4634709472881202
9	0.24033023042953514	0.39636817241558187
>10	2.532259907874892	10.524748883058836
>50	0.8329584066961728	11.09080182906845
>100	1.4071762802847327	52.229176434085986
>500	0.012424932562064124	1.5037116730015514
>1k	4.2118415464624146E-4	0.08084886320898885
>5k	2.1059207732312073E-4	0.2755807790813314
>10k+	2.1059207732312073E-4	1.3533983927156272
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35070	1.3335924211490189	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	7141	0.27154786083333743	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.047723367223895606	0.0
2	0.0	0.0	0.0	0.17165201565630658	0.0
3	0.0	0.0	0.0	0.23850275635718982	0.0
4	0.0	0.0	0.0	0.36125258057928944	0.0
5	0.0	0.0	0.0	0.6126083234876161	0.0
6	0.0	0.0	0.0	0.8307288289826481	0.0
7	0.0	0.0	0.0	0.988311007290077	0.0
8	0.0	0.0	0.0	1.4253505766161585	0.0
9	0.0	0.0	0.0	1.602896713324022	0.0
10	0.0	0.0	0.0	1.9230805794795605	0.0
11	0.0	0.0	0.0	2.1864907506030065	0.0
12	0.0	0.0	0.0	2.441344939554838	0.0
13	0.0	0.0	0.0	2.530098994615055	0.0
14	0.0	0.0	0.0	2.564132790364367	0.0
15	0.0	0.0	0.0	2.6241387453279583	0.0
16	0.0	0.0	0.0	2.7550642858473786	0.0
17	0.0	0.0	0.0	2.9172096546463355	0.0
18	0.0	0.0	0.0	3.1594390165716066	0.0
19	0.0	0.0	0.0	3.2680429502699697	0.0
20	0.0	0.0	0.0	3.3942531939481446	0.0
21	0.0	0.0	0.0	3.5446483472314174	0.0
22	0.0	0.0	0.0	3.712231518032778	0.0
23	0.0	0.0	0.0	3.885024331311967	0.0
24	0.0	0.0	0.0	4.010474043241554	0.0
25	0.0	0.0	0.0	4.113526095175224	0.0
26	0.0	0.0	0.0	4.227872043575427	0.0
27	0.0	0.0	0.0	4.350926080496962	0.0
28	0.0	0.0	0.0	4.4732956388447676	0.0
29	0.0	0.0	0.0	4.624033031414904	0.0
30	0.0	0.0	0.0	4.8023777264587855	0.0
31	0.0	0.0	0.0	4.947487184089372	0.0
32	0.0	0.0	0.0	5.079629575406533	0.0
33	0.0	0.0	0.0	5.219681496909009	0.0
34	0.0	0.0	0.0	5.386085843500059	0.0
35	0.0	0.0	0.0	5.574127318338435	0.0
36	0.0	0.0	0.0	5.730872911722418	0.0
37	0.0	0.0	0.0	5.895718168228862	0.0
38	0.0	0.0	0.0	6.096916842317812	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATTA	20	7.858333E-4	44.000004	42
GCGCATT	20	7.858333E-4	44.000004	41
CGTAGTC	30	2.5289646E-6	44.000004	13
CGCTATA	20	7.858333E-4	44.000004	16
CGGATAT	20	7.858333E-4	44.000004	32
CGCTAGT	20	7.858333E-4	44.000004	18
CTACCGT	20	7.858333E-4	44.000004	9
CGTACCC	20	7.858333E-4	44.000004	32
ATTACGC	50	2.7284841E-11	44.0	4
TATCGTG	70	0.0	44.0	1
TACGTCG	55	1.8189894E-12	44.0	1
TAGTACG	105	0.0	44.0	1
CGTTTTT	23705	0.0	43.164738	1
TACGACG	145	0.0	40.96552	1
TGCGTAG	255	0.0	40.54902	1
TATCACG	55	7.8216544E-11	40.0	1
CACGAAC	55	7.8216544E-11	40.0	31
TAATGCG	205	0.0	39.707317	1
ACTTACG	50	1.3496901E-9	39.600002	1
ACGACTA	95	0.0	39.36842	10
>>END_MODULE