FastQCFastQC Report
Thu 26 May 2016
SRR1545887_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545887_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1865929
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT244331.3094281722402086No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA31870.17079963921456817No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG207.8577443E-444.0000041
TAGCGAT207.8577443E-444.00000434
CGTAAGA207.8577443E-444.00000411
CAATCGT207.8577443E-444.00000425
TTCGACA207.8577443E-444.00000412
TACGTAC207.8577443E-444.00000412
GTCGACG454.802132E-1044.022
GTAGCGT302.52857E-644.011
TATCGGC351.4467332E-744.038
AATCGTC254.443912E-544.038
TACGTCG502.7284841E-1144.01
CGTTTTT155000.042.9354861
CGTTGAT1600.042.62500425
CGTAAGG2450.042.2040822
GCGTAAG800.041.2500041
TTCGTTC750.041.06666623
ACGTAGG1850.040.432432
TACGGGA7900.040.379754
CGCGATT603.6379788E-1240.33333240
TGTTACG603.6379788E-1240.3333321
Produced by FastQC (version 0.11.5)