##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545887_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1865929
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.573559336930828	31.0	31.0	33.0	30.0	34.0
2	31.979921529704505	31.0	31.0	34.0	30.0	34.0
3	32.01028281354757	33.0	31.0	34.0	30.0	34.0
4	35.779861934725275	37.0	35.0	37.0	35.0	37.0
5	35.7220451582027	37.0	35.0	37.0	33.0	37.0
6	35.76811282744413	37.0	35.0	37.0	33.0	37.0
7	36.040141398734896	37.0	35.0	37.0	35.0	37.0
8	36.03328744019735	37.0	35.0	37.0	35.0	37.0
9	37.82861244988421	39.0	38.0	39.0	35.0	39.0
10	37.36324158100335	39.0	37.0	39.0	34.0	39.0
11	37.084327431536785	39.0	37.0	39.0	33.0	39.0
12	36.41940181003672	38.0	35.0	39.0	33.0	39.0
13	36.14126957670951	38.0	35.0	39.0	32.0	39.0
14	37.10626127789428	39.0	35.0	41.0	32.0	41.0
15	37.30391349295713	39.0	35.0	41.0	32.0	41.0
16	37.44329178655779	39.0	35.0	41.0	33.0	41.0
17	37.38897353543463	39.0	35.0	41.0	33.0	41.0
18	37.25578250833767	39.0	35.0	41.0	32.0	41.0
19	37.1027139832223	38.0	35.0	41.0	32.0	41.0
20	36.868309029979166	38.0	35.0	41.0	32.0	41.0
21	36.74087224111957	38.0	35.0	41.0	32.0	41.0
22	36.670402785958096	38.0	35.0	41.0	32.0	41.0
23	36.59951691623851	38.0	35.0	40.0	32.0	41.0
24	36.643588796787014	38.0	35.0	41.0	32.0	41.0
25	36.61255438979725	38.0	35.0	41.0	32.0	41.0
26	36.38440262196472	38.0	35.0	40.0	31.0	41.0
27	36.36127366046618	38.0	35.0	40.0	31.0	41.0
28	36.37670190023307	38.0	35.0	40.0	31.0	41.0
29	36.316144397777194	38.0	35.0	40.0	31.0	41.0
30	36.18011832175822	38.0	35.0	40.0	31.0	41.0
31	35.936352347811734	38.0	35.0	40.0	30.0	41.0
32	35.67842774296342	38.0	35.0	40.0	30.0	41.0
33	35.385718320472	38.0	35.0	40.0	28.0	41.0
34	35.05033739225876	37.0	34.0	40.0	25.0	41.0
35	34.7754909216803	37.0	34.0	40.0	24.0	41.0
36	34.58141065388876	37.0	34.0	40.0	23.0	41.0
37	34.47949305680977	37.0	34.0	40.0	23.0	41.0
38	34.36986080392126	37.0	34.0	40.0	23.0	41.0
39	34.24335223901874	37.0	34.0	40.0	22.0	41.0
40	34.09075479292085	37.0	33.0	40.0	21.0	41.0
41	34.036462266249146	36.0	33.0	40.0	21.0	41.0
42	34.00659724994895	36.0	33.0	40.0	21.0	41.0
43	33.92545857854184	36.0	33.0	40.0	20.0	41.0
44	33.783265601209905	36.0	33.0	40.0	19.0	41.0
45	33.68527366260989	36.0	33.0	40.0	20.0	41.0
46	33.669395780868406	35.0	33.0	40.0	20.0	41.0
47	33.61595484072545	35.0	33.0	40.0	20.0	41.0
48	33.50016426134113	35.0	33.0	40.0	20.0	41.0
49	33.454686110779136	36.0	33.0	40.0	19.0	41.0
50	33.30813283892367	35.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	3.0
13	1.0
14	10.0
15	47.0
16	142.0
17	312.0
18	808.0
19	1580.0
20	2924.0
21	4743.0
22	7287.0
23	10753.0
24	16067.0
25	25145.0
26	36939.0
27	46127.0
28	47722.0
29	46324.0
30	48838.0
31	54783.0
32	65660.0
33	85634.0
34	150967.0
35	244189.0
36	119592.0
37	154329.0
38	243075.0
39	451927.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.18439715551878	21.793326541363577	27.841145081082935	18.181131222034708
2	34.82790609932104	23.990248289189996	28.56502042682224	12.616825184666727
3	27.317652493744404	23.44853421539619	36.076024328899976	13.157788961959433
4	25.019172755233456	25.657835855490752	35.8187798142373	13.504211575038493
5	22.17340531177767	30.817410523122795	33.45915091088675	13.550033254212781
6	20.365029966306327	38.91938010503079	30.7364856862185	9.979104242444379
7	83.90914123742115	3.623235396416477	11.16017812038936	1.307445245773017
8	85.41305698126777	2.5059367210649492	10.698370623962647	1.3826356737046266
9	81.05286964294997	4.739140663980248	12.061284218209803	2.1467054748599756
10	42.30809425224647	31.6174409637237	16.5161696934878	9.55829509054203
11	40.107045873664006	20.514124599596233	26.139043875731605	13.239785651008157
12	36.048424136180955	20.798379788298483	28.483720441667394	14.669475633853162
13	22.17560260867375	32.37893831973243	30.18646475830538	15.258994313288449
14	16.716445266674135	34.01592450730976	33.914044960981904	15.3535852650342
15	16.306247451001617	22.823805193016454	45.81128220848703	15.058665147494892
16	19.528556552794882	19.429463822042532	43.37640928459764	17.66557034056494
17	19.99068560486492	19.145905337234158	33.0664242851684	27.796984772732507
18	22.697648195617305	21.534688618913155	36.510713966072665	19.25694921939688
19	27.262505700913593	22.36687462384689	30.91977240291565	19.45084727232387
20	28.386449859560575	23.08051378160691	30.516970367039686	18.01606599179283
21	22.585639646524598	25.31302102062833	33.25887533770042	18.84246399514665
22	22.42872049258037	21.7532928637692	32.996539525351714	22.821447118298714
23	19.44859638282057	26.357058601908218	32.38461913609789	21.80972587917332
24	19.262630035762346	23.306728176688395	39.642987487734	17.787654299815266
25	19.060157165679936	23.640717304892096	37.519219648764775	19.779905880663197
26	19.012674115681786	29.213705344629943	31.20333088772402	20.57028965196425
27	17.979676611489502	28.921947190916697	34.268560057751394	18.829816139842407
28	16.757336425984054	24.934228472787552	39.42700928063179	18.881425820596604
29	17.820506568041978	23.52704738497553	38.485708727395306	20.166737319587185
30	20.28994672358916	26.773741123054524	34.4950960084762	18.44121614488011
31	26.58364814524025	23.346815446889995	31.23473615555576	18.834800252313993
32	26.788050349182633	25.086699440332406	30.938369037621477	17.18688117286349
33	25.854467131386027	25.250692818429854	29.421323105005605	19.473516945178517
34	20.116467454013524	26.28336876697881	32.128660844008536	21.471502934999133
35	20.384108934477144	25.809556526534504	33.460919466925056	20.3454150720633
36	27.251036882968215	26.081967749040825	28.076845367642605	18.590150000348352
37	21.144855993984766	30.494836620257253	29.508035943489812	18.85227144226817
38	21.39690202574696	30.905355991573096	26.77299082655342	20.92475115612652
39	21.331519044936865	29.036153036905475	28.63753122439278	20.994796693764876
40	23.881401703923355	25.609816879420382	28.423589536364997	22.085191880291266
41	19.265309666123418	24.92576084084657	29.24687916849998	26.562050324530034
42	21.905495868277946	24.985463005291198	28.172936912390554	24.9361042140403
43	21.74836234390483	25.854788687029355	28.56732490893276	23.829524060133046
44	20.59253058396113	28.186013508552577	30.371734401469723	20.849721506016575
45	19.205929057322116	32.22346616618317	26.967799953803173	21.60280482269154
46	21.388595171627646	29.715332148222146	28.865192619869244	20.030880060280964
47	20.645051339038087	26.95992184054163	29.83709455182914	22.55793226859114
48	21.99210152154771	25.13370015686556	31.900249152030973	20.973949169555755
49	22.13937400619209	24.33945771784457	32.160494852698044	21.3606734232653
50	19.49425728417319	30.566114787861704	28.93084356371545	21.008784364249657
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	500.0
1	1687.0
2	2874.0
3	23028.5
4	43183.0
5	30233.5
6	17284.0
7	17112.5
8	16941.0
9	17185.5
10	17430.0
11	17033.0
12	16636.0
13	15569.5
14	14503.0
15	13623.5
16	12744.0
17	11451.0
18	10158.0
19	9569.5
20	8981.0
21	9672.0
22	10363.0
23	9974.0
24	9585.0
25	10954.0
26	12323.0
27	14549.0
28	16775.0
29	21664.5
30	26554.0
31	28825.5
32	31097.0
33	38574.0
34	46051.0
35	50727.0
36	55403.0
37	59069.5
38	62736.0
39	75278.0
40	87820.0
41	109377.5
42	130935.0
43	153404.0
44	175873.0
45	170072.0
46	164271.0
47	152911.0
48	141551.0
49	133258.0
50	124965.0
51	117624.0
52	110283.0
53	108154.0
54	106025.0
55	96474.0
56	86923.0
57	79039.5
58	71156.0
59	68136.0
60	65116.0
61	59414.0
62	53712.0
63	47996.0
64	42280.0
65	33423.5
66	24567.0
67	21670.0
68	18773.0
69	15278.5
70	11784.0
71	9580.5
72	7377.0
73	6291.5
74	5206.0
75	3994.0
76	2782.0
77	2061.5
78	1341.0
79	853.5
80	366.0
81	358.5
82	351.0
83	252.5
84	154.0
85	140.0
86	126.0
87	82.0
88	38.0
89	29.0
90	20.0
91	11.0
92	2.0
93	2.0
94	2.0
95	3.5
96	5.0
97	4.5
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1865929.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.892139778687124
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.90962888376876	14.476463894300917
2	6.937635689899378	2.4825829499457632
3	2.2807540055988	1.2242270840692286
4	1.167335463655742	0.8354451713738833
5	0.7643539250540913	0.6837963633727973
6	0.5648351308927002	0.6063665468307138
7	0.4236139611445656	0.5305552143501334
8	0.32848677288025563	0.47018650046587085
9	0.2950499506237219	0.475116746242893
>10	3.5441026568377207	15.178630906874943
>50	1.3125144519466603	16.917315622046267
>100	1.4689603887093623	44.40835135138802
>500	0.0021223369909093323	0.2126496405534368
>1k	3.0319099870133317E-4	0.17288632766423548
>5k	0.0	0.0
>10k+	3.0319099870133317E-4	1.3254256805209494
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24433	1.3094281722402086	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3187	0.17079963921456817	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02551008103738138	0.0
2	0.0	0.0	0.0	0.09491250738908072	0.0
3	0.0	0.0	0.0	0.13864407488173452	0.0
4	0.0	0.0	0.0	0.1966848685024993	0.0
5	0.0	0.0	0.0	0.3241816810821848	0.0
6	0.0	0.0	0.0	0.43972734225150045	0.0
7	0.0	0.0	0.0	0.5199554752619204	0.0
8	0.0	0.0	0.0	0.7351297932557991	0.0
9	0.0	0.0	0.0	0.8251653733877334	0.0
10	0.0	0.0	0.0	0.9996093098933561	0.0
11	0.0	0.0	0.0	1.1557781673364849	0.0
12	0.0	0.0	0.0	1.3020859850508781	0.0
13	0.0	0.0	0.0	1.3549282957711681	0.0
14	0.0	0.0	0.0	1.3756150421586244	0.0
15	0.0	0.0	0.0	1.4090568290647716	0.0
16	0.0	0.0	0.0	1.4891777768607488	0.0
17	0.0	0.0	0.0	1.5804995795659964	0.0
18	0.0	0.0	0.0	1.7148026532627982	0.0
19	0.0	0.0	0.0	1.7822221531473061	0.0
20	5.3592607221389454E-5	0.0	0.0	1.8574125810789157	0.0
21	5.3592607221389454E-5	0.0	0.0	1.948144865104728	0.0
22	5.3592607221389454E-5	0.0	0.0	2.0526504491864372	0.0
23	5.3592607221389454E-5	0.0	0.0	2.1544764029070773	0.0
24	5.3592607221389454E-5	0.0	0.0	2.2369018328135746	0.0
25	5.3592607221389454E-5	0.0	0.0	2.306250666558052	0.0
26	5.3592607221389454E-5	0.0	0.0	2.3855141326384874	0.0
27	5.3592607221389454E-5	0.0	0.0	2.4789260470253693	0.0
28	5.3592607221389454E-5	0.0	0.0	2.555134734494185	0.0
29	5.3592607221389454E-5	0.0	0.0	2.654227465246534	0.0
30	5.3592607221389454E-5	0.0	0.0	2.7596977162582284	0.0
31	5.3592607221389454E-5	0.0	0.0	2.8642568929471595	0.0
32	5.3592607221389454E-5	0.0	0.0	2.9584191038351406	0.0
33	5.3592607221389454E-5	0.0	0.0	3.0494193508970597	0.0
34	5.3592607221389454E-5	0.0	0.0	3.1592841957009083	0.0
35	5.3592607221389454E-5	0.0	0.0	3.2783669689468358	0.0
36	5.3592607221389454E-5	0.0	0.0	3.379710589202483	0.0
37	5.3592607221389454E-5	0.0	0.0	3.4945595464779204	0.0
38	5.3592607221389454E-5	0.0	0.0	3.636311992578496	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATACG	20	7.8577443E-4	44.000004	1
TAGCGAT	20	7.8577443E-4	44.000004	34
CGTAAGA	20	7.8577443E-4	44.000004	11
CAATCGT	20	7.8577443E-4	44.000004	25
TTCGACA	20	7.8577443E-4	44.000004	12
TACGTAC	20	7.8577443E-4	44.000004	12
GTCGACG	45	4.802132E-10	44.0	22
GTAGCGT	30	2.52857E-6	44.0	11
TATCGGC	35	1.4467332E-7	44.0	38
AATCGTC	25	4.443912E-5	44.0	38
TACGTCG	50	2.7284841E-11	44.0	1
CGTTTTT	15500	0.0	42.935486	1
CGTTGAT	160	0.0	42.625004	25
CGTAAGG	245	0.0	42.204082	2
GCGTAAG	80	0.0	41.250004	1
TTCGTTC	75	0.0	41.066666	23
ACGTAGG	185	0.0	40.43243	2
TACGGGA	790	0.0	40.37975	4
CGCGATT	60	3.6379788E-12	40.333332	40
TGTTACG	60	3.6379788E-12	40.333332	1
>>END_MODULE