FastQCFastQC Report
Thu 26 May 2016
SRR1545886_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545886_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3181814
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT341271.0725642667987505No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA42870.1347344627938654No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTA254.4446217E-544.042
CGTTTTT210950.042.8632351
CGGTCTA4700.041.65957331
CGACGGT5000.038.7228
GGGCGAT39700.038.2921947
CTATGCG2300.038.260871
CGTAAGG4550.037.7142872
ACGTAAG1050.037.7142831
TACGGGA14900.037.2080544
TATAGCG2500.036.961
CGGGATT12000.036.856
ACGGGAT14400.036.6666685
TAGGGAT51900.036.6242755
ACGGGTA2650.036.52835
GTTTTTT259500.036.2936442
ATAGGGA46600.036.068674
ATAGCGG4700.036.0425532
GTAGGGA49450.035.947424
TAAGGGA42850.035.9393234
GCGGGAT19950.035.8396035
Produced by FastQC (version 0.11.5)