##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545886_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3181814
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.463876895381063	31.0	31.0	33.0	30.0	34.0
2	31.864537964821324	31.0	31.0	34.0	30.0	34.0
3	31.93168676735975	31.0	31.0	34.0	30.0	34.0
4	35.70248826612744	37.0	35.0	37.0	33.0	37.0
5	35.624879078412505	37.0	35.0	37.0	33.0	37.0
6	35.66685733358393	37.0	35.0	37.0	33.0	37.0
7	35.97151593399237	37.0	35.0	37.0	35.0	37.0
8	35.95777817307989	37.0	35.0	37.0	35.0	37.0
9	37.72807932833283	39.0	38.0	39.0	35.0	39.0
10	37.22142337672787	39.0	37.0	39.0	33.0	39.0
11	36.97779537081677	39.0	37.0	39.0	33.0	39.0
12	36.46040151938485	39.0	35.0	39.0	32.0	39.0
13	36.23824711312478	39.0	35.0	39.0	32.0	39.0
14	37.23699436862117	39.0	35.0	41.0	32.0	41.0
15	37.374826121200044	39.0	35.0	41.0	32.0	41.0
16	37.49982085690741	40.0	35.0	41.0	32.0	41.0
17	37.41716046255375	39.0	35.0	41.0	32.0	41.0
18	37.28728612043319	39.0	35.0	41.0	32.0	41.0
19	37.1781666055904	39.0	35.0	41.0	32.0	41.0
20	37.0047762062773	38.0	35.0	41.0	32.0	41.0
21	36.87478149256996	38.0	35.0	41.0	32.0	41.0
22	36.81452561337652	38.0	35.0	41.0	31.0	41.0
23	36.73175396173378	38.0	35.0	41.0	31.0	41.0
24	36.78887357966242	38.0	35.0	41.0	32.0	41.0
25	36.759874712978196	38.0	35.0	41.0	31.0	41.0
26	36.51260224513438	38.0	35.0	41.0	31.0	41.0
27	36.49932805625973	38.0	35.0	41.0	31.0	41.0
28	36.447708131273544	38.0	35.0	41.0	31.0	41.0
29	36.37510992157304	38.0	35.0	41.0	31.0	41.0
30	36.23430345079882	38.0	35.0	40.0	30.0	41.0
31	36.04488508756326	38.0	35.0	40.0	30.0	41.0
32	35.86735239709172	38.0	35.0	40.0	29.0	41.0
33	35.609320343678164	38.0	35.0	40.0	28.0	41.0
34	35.31578590074719	38.0	34.0	40.0	26.0	41.0
35	35.05249426899247	38.0	34.0	40.0	24.0	41.0
36	34.89451897565351	38.0	34.0	40.0	24.0	41.0
37	34.80661220297604	38.0	34.0	40.0	23.0	41.0
38	34.7014558361991	38.0	34.0	40.0	23.0	41.0
39	34.580930563508744	38.0	34.0	40.0	23.0	41.0
40	34.4606582911509	37.0	34.0	40.0	23.0	41.0
41	34.37213991766961	37.0	34.0	40.0	22.0	41.0
42	34.35028351751548	37.0	33.0	40.0	22.0	41.0
43	34.25540587853344	37.0	33.0	40.0	22.0	41.0
44	34.12916436975888	37.0	33.0	40.0	22.0	41.0
45	34.04914680745009	37.0	33.0	40.0	22.0	41.0
46	34.00646109420601	36.0	33.0	40.0	22.0	41.0
47	33.92977685056386	36.0	33.0	40.0	22.0	41.0
48	33.79685550443866	36.0	33.0	40.0	21.0	41.0
49	33.705423069984604	36.0	33.0	40.0	20.0	41.0
50	33.59108860543074	36.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	3.0
12	5.0
13	12.0
14	29.0
15	85.0
16	279.0
17	674.0
18	1443.0
19	2977.0
20	5229.0
21	8391.0
22	12794.0
23	18359.0
24	26715.0
25	40026.0
26	58054.0
27	72819.0
28	76631.0
29	77727.0
30	83515.0
31	95763.0
32	114547.0
33	145373.0
34	239679.0
35	343331.0
36	223833.0
37	291665.0
38	447603.0
39	794251.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.97967763043346	22.155631975973456	28.513671760825744	16.351018632767346
2	34.14228487271726	23.77338838788188	29.632404659731836	12.451922079669018
3	27.90367381625701	24.12963171323025	35.17317479902974	12.793519671482997
4	25.451330593177353	26.422820441421152	33.872281660713035	14.253567304688458
5	22.4683466726842	30.853877693667826	32.71357156640835	13.96420406723963
6	20.629521398799554	39.050459894890146	30.27169407136935	10.048324634940949
7	85.0303317541503	3.4154416317232874	9.956395942691811	1.597830671434597
8	85.9924873044119	2.58773768674096	9.670426995418337	1.7493480134288177
9	80.85208626274193	5.283181229323901	11.131323201167636	2.7334093067665175
10	39.14443144696704	34.11591626663281	16.54160174039086	10.19805054600929
11	36.47347079370447	21.540542596141698	26.828940975179567	15.157045634974263
12	33.68814141870015	20.417063976712654	29.402818643704503	16.49197596088269
13	24.66266098521158	27.27362441676352	30.472742907033535	17.590971690991367
14	18.62359019100425	29.540255967193556	33.75505293521243	18.081100906589764
15	18.21090107718427	23.43798223277665	41.318474304280514	17.032642385758564
16	22.329275061332936	21.039790509438955	38.29897033578958	18.331964093438526
17	22.02567466231527	20.7867587483115	31.72979313058526	25.457773458787976
18	23.345770683012898	22.549212493250707	34.066447630188314	20.038569193548085
19	26.227931613852977	24.268954753483392	29.627156081405136	19.875957551258498
20	27.39732743648749	24.02761443629326	29.673890428541704	18.901167698677547
21	24.367012025215804	24.4721093062008	32.202102322763054	18.95877634582034
22	23.54474523023659	22.198909175709204	31.96981344604053	22.28653214801368
23	20.97891328657175	25.147164479130456	31.35139263325889	22.522529601038904
24	21.71299139421726	23.515799477907887	35.411655112461006	19.35955401541385
25	21.016627621853445	24.273103330364375	33.19446076986273	21.51580827791945
26	20.486175496116367	27.242604375994322	30.5874887721281	21.68373135576121
27	20.074649240967574	26.5446063157683	32.12359364815165	21.257150795112473
28	18.99073924497158	25.422133411946767	34.91018016766536	20.676947175416288
29	21.08900771698157	23.77596553412613	34.54340825705085	20.591618491841444
30	22.577906816677533	24.712726765298036	32.817505988722154	19.891860429302277
31	25.317381845701853	24.716403912988	30.079539533109102	19.886674708201046
32	26.992684047527604	24.33344626681509	29.642461815806957	19.03140786985034
33	26.19323442539382	25.393093373779863	28.89213511537758	19.52153708544874
34	21.651454170482623	26.192102995335365	31.701727379413125	20.454715454768884
35	21.634671291282267	26.51201484436237	31.10210087704687	20.7512129873085
36	25.707630930029225	26.66796362075219	27.89701095035725	19.727394498861344
37	22.53123532676643	29.050912466913527	28.971586648371023	19.44626555794902
38	22.183760584371054	29.124392563487365	26.884978191685622	21.80686866045595
39	21.877300181594524	28.52872606632569	27.919293836786185	21.674679915293606
40	23.877228524357488	26.423480442288582	27.855210895420036	21.844080137933897
41	20.62433567769832	25.058944363184022	28.942452324365913	25.374267634751746
42	22.094503324204368	25.871028287637177	27.986393924974873	24.048074463183582
43	22.24589495174765	25.60573936754317	28.722703464124557	23.425662216584627
44	21.551542610598858	26.834158124893538	29.040321024421917	22.573978240085687
45	20.29722667635506	29.422964384467477	27.577696244972206	22.702112694205255
46	21.860768731296044	28.283677172832856	28.663963386923307	21.19159070894779
47	21.531868299026907	26.91804109228258	29.641141814072096	21.908948794618414
48	22.498298140620413	25.077172958570177	30.57683447241102	21.84769442839839
49	21.754005733836106	24.706503899976553	31.298498278026308	22.24099208816103
50	20.52225554353586	27.59334769411411	29.936539345165997	21.947857417184032
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	864.0
1	3134.5
2	5405.0
3	34761.5
4	64118.0
5	44811.0
6	25504.0
7	24704.5
8	23905.0
9	23962.5
10	24020.0
11	22919.5
12	21819.0
13	20680.0
14	19541.0
15	17712.0
16	15883.0
17	14826.5
18	13770.0
19	13752.5
20	13735.0
21	14839.0
22	15943.0
23	16848.5
24	17754.0
25	20892.0
26	24030.0
27	27834.5
28	31639.0
29	38328.0
30	45017.0
31	52855.5
32	60694.0
33	67645.5
34	74597.0
35	81960.5
36	89324.0
37	97456.0
38	105588.0
39	122567.5
40	139547.0
41	163067.5
42	186588.0
43	216419.5
44	246251.0
45	247310.5
46	248370.0
47	246068.5
48	243767.0
49	232089.0
50	220411.0
51	211746.5
52	203082.0
53	196126.5
54	189171.0
55	179120.5
56	169070.0
57	163352.0
58	157634.0
59	150081.5
60	142529.0
61	125565.5
62	108602.0
63	96620.5
64	84639.0
65	69956.0
66	55273.0
67	45845.5
68	36418.0
69	29866.0
70	23314.0
71	19371.0
72	15428.0
73	12174.0
74	8920.0
75	6948.0
76	4976.0
77	3674.0
78	2372.0
79	1764.0
80	1156.0
81	885.5
82	615.0
83	466.0
84	317.0
85	212.0
86	107.0
87	81.5
88	56.0
89	44.5
90	33.0
91	20.5
92	8.0
93	7.5
94	7.0
95	4.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3181814.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.247200799990214
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.51312587378372	14.508919740538287
2	7.598711874814502	2.7731044280202064
3	2.6022412169658438	1.4245085404795996
4	1.3079193151707664	0.9546346549642664
5	0.8099199492042545	0.7389385972523953
6	0.5699363265558026	0.6239845556323514
7	0.44726803724773845	0.571297278095389
8	0.33973740439176464	0.49594053097632046
9	0.30397817457045717	0.4992075711181449
>10	3.716185576025692	16.535281832005836
>50	1.4532815292347374	19.212157950193756
>100	1.3320998899503378	39.72276049928283
>500	0.004370962566069548	0.5245832102120394
>1k	0.0010490310158566916	0.32592240318227783
>5k	0.0	0.0
>10k+	1.748385026427819E-4	1.0887582080463583
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34127	1.0725642667987505	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	4287	0.1347344627938654	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.050128637311923324	0.0
2	0.0	0.0	0.0	0.16905450790021037	0.0
3	0.0	0.0	0.0	0.23621745331436722	0.0
4	0.0	0.0	0.0	0.32465756954994857	0.0
5	0.0	0.0	0.0	0.5317092702464694	0.0
6	0.0	0.0	0.0	0.712235221794863	0.0
7	0.0	0.0	0.0	0.8381068157975293	0.0
8	0.0	0.0	0.0	1.1479929373621462	0.0
9	0.0	0.0	0.0	1.2652845200882263	0.0
10	0.0	0.0	0.0	1.4843733794621559	0.0
11	3.1428612734748165E-5	0.0	0.0	1.7070765293005814	0.0
12	3.1428612734748165E-5	0.0	0.0	1.909539652537829	0.0
13	3.1428612734748165E-5	0.0	0.0	1.9910026167462962	0.0
14	3.1428612734748165E-5	0.0	0.0	2.0260455199455407	0.0
15	3.1428612734748165E-5	0.0	0.0	2.0827427373190264	0.0
16	3.1428612734748165E-5	0.0	0.0	2.199971462819637	0.0
17	3.1428612734748165E-5	0.0	0.0	2.342720221860863	0.0
18	3.1428612734748165E-5	0.0	0.0	2.537514763590832	0.0
19	3.1428612734748165E-5	0.0	0.0	2.635383463646838	0.0
20	3.1428612734748165E-5	0.0	0.0	2.7429950336506157	0.0
21	3.1428612734748165E-5	0.0	0.0	2.8753409218766404	0.0
22	3.1428612734748165E-5	0.0	0.0	3.026858263870861	0.0
23	3.1428612734748165E-5	0.0	0.0	3.1883384761019973	0.0
24	3.1428612734748165E-5	0.0	0.0	3.3090557776161647	0.0
25	6.285722546949633E-5	0.0	0.0	3.4148130594685924	0.0
26	6.285722546949633E-5	0.0	0.0	3.52374463120723	0.0
27	6.285722546949633E-5	0.0	0.0	3.645090504976092	0.0
28	6.285722546949633E-5	0.0	0.0	3.764299233078992	0.0
29	6.285722546949633E-5	0.0	0.0	3.9039679880722127	0.0
30	6.285722546949633E-5	0.0	0.0	4.060765337005871	0.0
31	6.285722546949633E-5	0.0	0.0	4.201156950091992	0.0
32	6.285722546949633E-5	0.0	0.0	4.328442831667721	0.0
33	6.285722546949633E-5	0.0	0.0	4.466665870475144	0.0
34	6.285722546949633E-5	0.0	0.0	4.615606066225116	0.0
35	6.285722546949633E-5	0.0	0.0	4.788337721815291	0.0
36	6.285722546949633E-5	0.0	0.0	4.94381506901409	0.0
37	6.285722546949633E-5	0.0	0.0	5.102906706677386	0.0
38	6.285722546949633E-5	0.0	0.0	5.288618379327013	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTCTA	25	4.4446217E-5	44.0	42
CGTTTTT	21095	0.0	42.863235	1
CGGTCTA	470	0.0	41.659573	31
CGACGGT	500	0.0	38.72	28
GGGCGAT	3970	0.0	38.292194	7
CTATGCG	230	0.0	38.26087	1
CGTAAGG	455	0.0	37.714287	2
ACGTAAG	105	0.0	37.714283	1
TACGGGA	1490	0.0	37.208054	4
TATAGCG	250	0.0	36.96	1
CGGGATT	1200	0.0	36.85	6
ACGGGAT	1440	0.0	36.666668	5
TAGGGAT	5190	0.0	36.624275	5
ACGGGTA	265	0.0	36.5283	5
GTTTTTT	25950	0.0	36.293644	2
ATAGGGA	4660	0.0	36.06867	4
ATAGCGG	470	0.0	36.042553	2
GTAGGGA	4945	0.0	35.94742	4
TAAGGGA	4285	0.0	35.939323	4
GCGGGAT	1995	0.0	35.839603	5
>>END_MODULE