FastQCFastQC Report
Thu 26 May 2016
SRR1545885_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545885_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2831797
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT318641.125221899733632No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA44570.15739122543035394No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA31360.11074240137975992No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA207.858435E-444.00000432
ACGATTC351.447097E-744.029
GTACGTC302.5290356E-644.038
CGTTTTT194500.042.9933171
TATAGCG1500.042.5333331
TATACGG3850.041.1428572
CGGTCTA3750.040.4831
CGACAGG3050.040.3934442
TACGGGC3900.040.051284
TAGAACG1000.039.6000021
ATACGGG8600.038.8837243
GCGATCG404.127396E-738.5000049
AGGGCGC5150.038.4465986
TAGGGCG9800.038.1632655
GGTCGAC750.038.133339
CGGGATA6300.038.063496
ACGGGAT11550.037.7142875
TTAGCGG8000.037.6750032
GGGCGAT41100.037.5231137
CGTAAGG4400.037.52
Produced by FastQC (version 0.11.5)