FastQCFastQC Report
Thu 26 May 2016
SRR1545884_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545884_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences434627
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37790.8694811873169407No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA7840.18038455963389297No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGACG207.8510714E-444.0000041
GTACCGG207.8510714E-444.0000042
CGACGTC207.8510714E-444.00000418
CGCATTA207.8510714E-444.00000415
ATCAGCG207.8510714E-444.0000041
TAAGTCC207.8510714E-444.00000421
CACGACC207.8510714E-444.00000427
TGAAACG207.8510714E-444.00000413
GCCGACC207.8510714E-444.00000414
TATTACG207.8510714E-444.0000041
CGCGTAA207.8510714E-444.00000431
CGCAAAA207.8510714E-444.00000414
ACTCACC207.8510714E-444.00000426
CCACGAG207.8510714E-444.00000440
GTAGTCA207.8510714E-444.00000418
ACGCGAG254.4382567E-544.01
ACTAGCT254.4382567E-544.022
CTACGAC302.5240697E-644.013
TACGACC302.5240697E-644.014
ACGCTTA254.4382567E-544.010
Produced by FastQC (version 0.11.5)