##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545884_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	434627
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.524401383255068	31.0	31.0	33.0	30.0	34.0
2	31.938874023012836	31.0	31.0	34.0	30.0	34.0
3	32.0158204621434	33.0	31.0	34.0	30.0	34.0
4	35.772703950743974	37.0	35.0	37.0	35.0	37.0
5	35.69484868634484	37.0	35.0	37.0	33.0	37.0
6	35.74837274260458	37.0	35.0	37.0	33.0	37.0
7	35.99307912301813	37.0	35.0	37.0	35.0	37.0
8	35.98131271181956	37.0	35.0	37.0	35.0	37.0
9	37.78302314398323	39.0	38.0	39.0	35.0	39.0
10	37.32408709076979	39.0	37.0	39.0	34.0	39.0
11	37.03323539494785	39.0	37.0	39.0	33.0	39.0
12	36.28880166211487	38.0	35.0	39.0	32.0	39.0
13	35.96711893186571	38.0	35.0	39.0	32.0	39.0
14	37.0141707717192	39.0	35.0	41.0	32.0	41.0
15	37.19538822944732	39.0	35.0	41.0	32.0	41.0
16	37.34833086761752	39.0	35.0	41.0	33.0	41.0
17	37.26594988346329	39.0	35.0	41.0	32.0	41.0
18	37.15489603729175	39.0	35.0	41.0	32.0	41.0
19	37.033633437407246	38.0	35.0	41.0	32.0	41.0
20	36.853131535776654	38.0	35.0	41.0	32.0	41.0
21	36.68952688167095	38.0	35.0	41.0	32.0	41.0
22	36.60596097343239	38.0	35.0	41.0	32.0	41.0
23	36.54968743313232	38.0	35.0	40.0	31.0	41.0
24	36.586700780209235	38.0	35.0	41.0	32.0	41.0
25	36.534439876031634	38.0	35.0	40.0	32.0	41.0
26	36.316689943330715	38.0	35.0	40.0	31.0	41.0
27	36.297280196582356	38.0	35.0	40.0	31.0	41.0
28	36.299323327819025	38.0	35.0	40.0	31.0	41.0
29	36.27532803990548	38.0	35.0	40.0	31.0	41.0
30	36.18479063656883	38.0	35.0	40.0	31.0	41.0
31	35.99000752369273	38.0	35.0	40.0	30.0	41.0
32	35.7588967091322	38.0	35.0	40.0	30.0	41.0
33	35.49301585037285	38.0	35.0	40.0	29.0	41.0
34	35.24680243058991	37.0	34.0	40.0	27.0	41.0
35	34.99112112224965	37.0	34.0	40.0	25.0	41.0
36	34.80694710636937	37.0	34.0	40.0	24.0	41.0
37	34.72557158206922	37.0	34.0	40.0	24.0	41.0
38	34.66894601577905	37.0	34.0	40.0	24.0	41.0
39	34.61121145257888	37.0	34.0	40.0	23.0	41.0
40	34.483241952294726	37.0	34.0	40.0	23.0	41.0
41	34.44173049534428	37.0	34.0	40.0	23.0	41.0
42	34.40461591203492	36.0	34.0	40.0	23.0	41.0
43	34.31198475934537	36.0	33.0	40.0	23.0	41.0
44	34.1922591095353	36.0	34.0	40.0	23.0	41.0
45	34.091984621295964	36.0	33.0	40.0	23.0	41.0
46	34.044166607228725	36.0	33.0	40.0	23.0	41.0
47	34.0130617748092	35.0	33.0	40.0	23.0	41.0
48	33.95286533050179	35.0	33.0	40.0	23.0	41.0
49	33.891332107761365	36.0	33.0	40.0	23.0	41.0
50	33.73378782266173	35.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	2.0
15	14.0
16	20.0
17	90.0
18	179.0
19	365.0
20	642.0
21	1095.0
22	1601.0
23	2441.0
24	3282.0
25	5197.0
26	7773.0
27	9650.0
28	10261.0
29	10018.0
30	10903.0
31	12602.0
32	15363.0
33	20046.0
34	36836.0
35	61759.0
36	27291.0
37	35678.0
38	56902.0
39	104616.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.219183345719433	23.770037296348363	28.207865595096486	16.80291376283572
2	33.009224001270056	24.29025348172111	29.77380604518357	12.926716471825268
3	27.581351365653767	24.64412013059474	34.544333416929916	13.230195086821572
4	24.547715627423056	26.885352267576568	34.1536535926208	14.413278512379582
5	22.521840566738835	31.15982210032971	32.36775441930667	13.950582913624787
6	20.757799216339528	40.26072931502185	28.99037565544709	9.991095813191542
7	85.63756968619069	3.6969631431089187	9.276460045050124	1.3890071256502703
8	87.77457452022078	2.0751126828291846	8.706315990492998	1.443996806457031
9	83.17177717905238	4.88050673335987	10.094402786757382	1.853313300830367
10	45.058176321305396	32.64569389384461	13.799188729646342	8.496941055203658
11	42.82752797226127	19.70011066960865	23.774638943277797	13.697722414852276
12	38.875863671608066	19.95895330938943	26.185901934302287	14.979281084700213
13	21.91465325439975	35.84176776868441	26.094789325099455	16.148789651816383
14	15.43852544825793	36.762787401611035	30.584616234150204	17.21407091598083
15	15.35178440363806	22.67185425663845	46.861101588258435	15.115259751465048
16	18.362181824875123	19.02090758282389	43.977709622273814	18.639200970027172
17	18.424304058422507	18.892521633492628	30.61866842142803	32.06450588665683
18	21.744852482703557	22.538636578031277	35.44901720325704	20.26749373600812
19	26.804593363964962	23.38672010712634	29.002339937463617	20.806346591445077
20	30.217174726834735	22.01404882807557	28.4533634587819	19.315412986307802
21	22.42497589887421	25.05343662496808	30.97115457622283	21.550432899934886
22	22.664491621551353	22.96382875431117	29.536821228317617	24.834858395819865
23	20.394039026567608	27.324349384644762	28.352357308680777	23.92925428010685
24	20.803355520940944	22.058684803291097	38.88828811831754	18.249671557450412
25	17.84127539246296	24.68461462357378	36.109123455284646	21.364986528678614
26	19.005952230303226	30.509379306853923	29.81821193805263	20.66645652479022
27	18.309032802840136	30.089018859849944	31.460309644821884	20.141638692488044
28	16.331705117261468	26.42679815105826	39.08569877159035	18.155797960089917
29	17.664342068026144	22.68174779753674	38.19021827912209	21.463691855315016
30	19.43528588881961	26.860043209464667	33.51540516350802	20.189265738207705
31	26.883971773497734	25.419497638204714	27.922333403124977	19.774197185172575
32	27.761275760594717	25.07920584777292	29.170530132734505	17.98898825889786
33	25.74391374672996	27.11060288477246	27.35034868979608	19.795134678701505
34	20.790470909538524	26.654119509372403	29.69097640045372	22.86443318063535
35	20.72627793487289	25.562608857710174	31.37080761204435	22.340305595372584
36	27.87102503986177	25.49933621243043	27.17985767106047	19.449781076647334
37	20.917246282444488	30.289190501280412	29.915076605917257	18.878486610357847
38	20.838788202297604	29.79405329167309	26.892254737970724	22.47490376805859
39	21.6914733783221	29.404753961442797	28.246289346957276	20.657483313277822
40	23.55007857312132	25.82260190922331	27.57674971872433	23.050569798931036
41	18.219530770062605	25.48115970705916	28.54930779726064	27.7500017256176
42	22.552441518819585	25.16870788055045	26.154150570489175	26.124700030140787
43	22.92241393194624	26.39044514031572	26.678278155751944	24.008862771986095
44	20.108966999289045	29.827875396604448	28.50191083388745	21.56124677021906
45	18.65714739305197	33.95325186884386	25.003048591090753	22.38655214701342
46	21.69515469586565	30.18979492760459	27.829380135150373	20.28567024137939
47	20.835336967100528	27.201255329282347	28.849105094713394	23.114302608903728
48	22.5009030732099	23.695030451398555	31.561315794923	22.24275068046854
49	20.739622710968256	24.574405179613784	31.69890503811314	22.98706707130482
50	18.88999072768144	31.430168857434076	28.062223469779834	21.617616945104654
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	101.0
1	303.0
2	505.0
3	3912.5
4	7320.0
5	5272.5
6	3225.0
7	3244.0
8	3263.0
9	3338.5
10	3414.0
11	3292.0
12	3170.0
13	2983.5
14	2797.0
15	2564.0
16	2331.0
17	2197.0
18	2063.0
19	1846.5
20	1630.0
21	1745.0
22	1860.0
23	1857.0
24	1854.0
25	2128.5
26	2403.0
27	3189.5
28	3976.0
29	4614.5
30	5253.0
31	6665.5
32	8078.0
33	9009.5
34	9941.0
35	10975.0
36	12009.0
37	12519.0
38	13029.0
39	16518.0
40	20007.0
41	25851.0
42	31695.0
43	36196.0
44	40697.0
45	40646.5
46	40596.0
47	37860.5
48	35125.0
49	33048.5
50	30972.0
51	30058.0
52	29144.0
53	26271.0
54	23398.0
55	21007.5
56	18617.0
57	18268.5
58	17920.0
59	17494.0
60	17068.0
61	15264.0
62	13460.0
63	11606.0
64	9752.0
65	8179.0
66	6606.0
67	5388.5
68	4171.0
69	3593.5
70	3016.0
71	2425.5
72	1835.0
73	1487.0
74	1139.0
75	872.0
76	605.0
77	458.5
78	312.0
79	240.0
80	168.0
81	106.5
82	45.0
83	37.5
84	30.0
85	23.0
86	16.0
87	12.0
88	8.0
89	5.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	434627.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.706366608609223
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.49144779507134	17.14136489449574
2	5.491042477302205	2.493632471061393
3	2.4146806095979247	1.6448586949269144
4	1.5888456549935148	1.4430764770711437
5	1.2453388456549936	1.4138560190692249
6	0.9758025291828794	1.3294157979140735
7	0.8339413099870299	1.3255043980240528
8	0.783276588845655	1.422829230581625
9	0.7042396238651103	1.4391650771811233
>10	9.078104734111543	48.83934960322299
>50	1.3507214656290532	19.385818184328173
>100	0.04053177691309987	1.0712634051727112
>500	0.0010132944228274966	0.18038455963389297
>1k	0.0010132944228274966	0.8694811873169407
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3779	0.8694811873169407	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	784	0.18038455963389297	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02254806995423662	0.0
2	0.0	0.0	0.0	0.09364351501402352	0.0
3	0.0	0.0	0.0	0.13758924319013774	0.0
4	0.0	0.0	0.0	0.20707411182462204	0.0
5	0.0	0.0	0.0	0.34742434317242143	0.0
6	0.0	0.0	0.0	0.47143872792072283	0.0
7	0.0	0.0	0.0	0.55150738449291	0.0
8	0.0	0.0	0.0	0.7733067664917274	0.0
9	0.0	0.0	0.0	0.8536055054103864	0.0
10	0.0	0.0	0.0	1.003159030617058	0.0
11	0.0	0.0	0.0	1.143739344311329	0.0
12	0.0	0.0	0.0	1.2827090815802975	0.0
13	0.0	0.0	0.0	1.3340174448435096	0.0
14	0.0	0.0	0.0	1.3517337855218383	0.0
15	0.0	0.0	0.0	1.3864762198390803	0.0
16	0.0	0.0	0.0	1.4605627354030006	0.0
17	0.0	0.0	0.0	1.5512151799128908	0.0
18	0.0	0.0	0.0	1.6947865641112954	0.0
19	0.0	0.0	0.0	1.7435640215633175	0.0
20	0.0	0.0	0.0	1.8093675726542529	0.0
21	0.0	0.0	0.0	1.8928874644235172	0.0
22	0.0	0.0	0.0	1.986300897091069	0.0
23	0.0	0.0	0.0	2.082705400262754	0.0
24	0.0	0.0	0.0	2.1659952096855464	0.0
25	0.0	0.0	0.0	2.227887360886461	0.0
26	0.0	0.0	0.0	2.301513711757438	0.0
27	0.0	0.0	0.0	2.3659367687695427	0.0
28	0.0	0.0	0.0	2.4437046018770117	0.0
29	0.0	0.0	0.0	2.527224493646276	0.0
30	0.0	0.0	0.0	2.6300713025191715	0.0
31	0.0	0.0	0.0	2.7200334999896465	0.0
32	0.0	0.0	0.0	2.806544462263044	0.0
33	0.0	0.0	0.0	2.8919050128040826	0.0
34	0.0	0.0	0.0	2.9837078690463317	0.0
35	0.0	0.0	0.0	3.0969083835104585	0.0
36	0.0	0.0	0.0	3.195843792493333	0.0
37	0.0	0.0	0.0	3.2832750841526184	0.0
38	0.0	0.0	0.0	3.3709364581583747	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTGACG	20	7.8510714E-4	44.000004	1
GTACCGG	20	7.8510714E-4	44.000004	2
CGACGTC	20	7.8510714E-4	44.000004	18
CGCATTA	20	7.8510714E-4	44.000004	15
ATCAGCG	20	7.8510714E-4	44.000004	1
TAAGTCC	20	7.8510714E-4	44.000004	21
CACGACC	20	7.8510714E-4	44.000004	27
TGAAACG	20	7.8510714E-4	44.000004	13
GCCGACC	20	7.8510714E-4	44.000004	14
TATTACG	20	7.8510714E-4	44.000004	1
CGCGTAA	20	7.8510714E-4	44.000004	31
CGCAAAA	20	7.8510714E-4	44.000004	14
ACTCACC	20	7.8510714E-4	44.000004	26
CCACGAG	20	7.8510714E-4	44.000004	40
GTAGTCA	20	7.8510714E-4	44.000004	18
ACGCGAG	25	4.4382567E-5	44.0	1
ACTAGCT	25	4.4382567E-5	44.0	22
CTACGAC	30	2.5240697E-6	44.0	13
TACGACC	30	2.5240697E-6	44.0	14
ACGCTTA	25	4.4382567E-5	44.0	10
>>END_MODULE