FastQCFastQC Report
Thu 26 May 2016
SRR1545883_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545883_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1956901
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT343631.7559907220651427No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA43130.22039949900378197No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGC408.3182385E-944.021
CGAACCC207.8578404E-444.023
ACCATCG207.8578404E-444.025
CGTGATA254.443991E-544.021
GACGTAA302.5286317E-644.017
ATTACGC207.8578404E-444.044
GCACGTC207.8578404E-444.027
TTGTACG207.8578404E-444.01
CGCGACT302.5286317E-644.041
CGTTACA254.443991E-544.030
TGTTGCG900.044.01
ACCTACG600.044.015
CGTAGCA254.443991E-544.044
CGCTAAT351.4467878E-744.044
TCGCTAC302.5286317E-644.015
AGCGCGA351.4467878E-744.043
CGAATCA207.8578404E-444.030
ACGTCCC254.443991E-544.027
TTACGCG700.044.01
CAGTCGA207.8578404E-444.019
Produced by FastQC (version 0.11.5)