FastQCFastQC Report
Thu 26 May 2016
SRR1545882_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545882_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1654376
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208241.2587223218905497No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA39600.239365174543151No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGA454.802132E-1044.08
CGAACGT207.8574865E-444.015
CGTGCTA207.8574865E-444.018
ACCGCGT207.8574865E-444.023
CGACCGT207.8574865E-444.019
CGCGAGA207.8574865E-444.020
CGACAGG1400.044.02
CCGCTAG207.8574865E-444.032
CCGTAGT254.443691E-544.041
TAACCGA254.443691E-544.039
CTACGAC207.8574865E-444.013
TACGACA302.5283953E-644.028
GCGATCG302.5283953E-644.09
TTACGCG302.5283953E-644.01
ATACGAC302.5283953E-644.027
CGTCCAT207.8574865E-444.015
CGTCACG207.8574865E-444.021
TATCGAT254.443691E-544.029
TAATGCG454.802132E-1044.01
AATACGA207.8574865E-444.021
Produced by FastQC (version 0.11.5)