##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545882_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1654376
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.508490814663656	31.0	31.0	33.0	30.0	34.0
2	31.90194671586145	31.0	31.0	34.0	30.0	34.0
3	31.890528513469732	31.0	31.0	34.0	30.0	34.0
4	35.68005036339986	37.0	35.0	37.0	33.0	37.0
5	35.62924752293312	37.0	35.0	37.0	33.0	37.0
6	35.68975371983153	37.0	35.0	37.0	33.0	37.0
7	36.02108287354265	37.0	35.0	37.0	35.0	37.0
8	36.02162386301542	37.0	35.0	37.0	35.0	37.0
9	37.82330256241628	39.0	38.0	39.0	35.0	39.0
10	37.31438016508944	39.0	37.0	39.0	34.0	39.0
11	36.99142395682723	39.0	37.0	39.0	33.0	39.0
12	36.43975069754397	38.0	35.0	39.0	32.0	39.0
13	36.19402662998012	38.0	35.0	39.0	32.0	39.0
14	37.26582892885293	39.0	35.0	41.0	32.0	41.0
15	37.41831361189959	39.0	35.0	41.0	32.0	41.0
16	37.53133447293723	39.0	35.0	41.0	33.0	41.0
17	37.45540977383618	39.0	35.0	41.0	33.0	41.0
18	37.26564880051451	39.0	35.0	41.0	32.0	41.0
19	37.08518680154935	38.0	35.0	41.0	32.0	41.0
20	36.843346977954226	38.0	35.0	41.0	32.0	41.0
21	36.716245279186836	38.0	35.0	40.0	32.0	41.0
22	36.607577721146825	38.0	35.0	40.0	32.0	41.0
23	36.52581819368753	38.0	35.0	40.0	31.0	41.0
24	36.56664204509737	38.0	35.0	40.0	32.0	41.0
25	36.5287407457555	38.0	35.0	40.0	32.0	41.0
26	36.29641024773087	38.0	35.0	40.0	31.0	41.0
27	36.28483246855612	38.0	35.0	40.0	31.0	41.0
28	36.268711586725146	38.0	35.0	40.0	31.0	41.0
29	36.2470599186642	38.0	35.0	40.0	31.0	41.0
30	36.10344927634347	38.0	35.0	40.0	31.0	41.0
31	35.853689245975524	38.0	35.0	40.0	30.0	41.0
32	35.53267999535777	38.0	35.0	40.0	29.0	41.0
33	35.15031770286803	38.0	34.0	40.0	26.0	41.0
34	34.75029195297804	37.0	34.0	40.0	23.0	41.0
35	34.39972654342181	37.0	34.0	40.0	22.0	41.0
36	34.17436302267441	37.0	33.0	40.0	20.0	41.0
37	34.057373898073955	37.0	33.0	40.0	20.0	41.0
38	33.96783862918708	37.0	33.0	40.0	18.0	41.0
39	33.85520341204176	37.0	33.0	40.0	18.0	41.0
40	33.73378180050968	37.0	33.0	40.0	18.0	41.0
41	33.68070559534229	37.0	33.0	40.0	18.0	41.0
42	33.638284767187145	37.0	33.0	40.0	18.0	41.0
43	33.523645773391294	36.0	33.0	40.0	17.0	41.0
44	33.368147265192434	36.0	33.0	40.0	16.0	41.0
45	33.26079682006992	36.0	33.0	40.0	17.0	41.0
46	33.19877827047781	36.0	33.0	40.0	16.0	41.0
47	33.1333669008738	36.0	32.0	40.0	15.0	41.0
48	33.02740731248519	35.0	32.0	40.0	15.0	41.0
49	32.9509168411534	36.0	32.0	40.0	15.0	41.0
50	32.82601899447284	35.0	32.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	2.0
13	1.0
14	18.0
15	34.0
16	79.0
17	304.0
18	712.0
19	1421.0
20	2687.0
21	4379.0
22	6864.0
23	10475.0
24	15822.0
25	25512.0
26	38723.0
27	47695.0
28	46733.0
29	43897.0
30	44821.0
31	49887.0
32	58638.0
33	74913.0
34	126980.0
35	207505.0
36	106272.0
37	135342.0
38	214632.0
39	390026.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.466471950753636	22.071947368675563	28.69057578204713	18.77100489852367
2	35.5043835258732	23.698361194794895	28.87596290081578	11.92129237851613
3	25.81347891893983	23.570397539616145	38.55435523726166	12.061768304182362
4	23.47035982146743	26.262349066959384	37.11405387892474	13.153237232648443
5	21.242087651174824	30.688670531970967	35.26961222841724	12.79962958843697
6	19.51460852913727	38.60772883552469	31.839799416819393	10.037863218518645
7	82.59071698332181	3.1148300023694735	12.85808062979637	1.4363723845123477
8	84.07042897140674	2.303285347466356	12.27544403448793	1.3508416466389745
9	79.8681194601469	4.724802584176753	13.555564152284608	1.851513803391732
10	40.29785248335324	34.04328882914162	17.46561845674744	8.193240230757699
11	35.335921217425785	19.272341958539048	31.756686508991912	13.635050315043253
12	32.6857377041253	20.394094208329907	32.24956116384667	14.67060692369812
13	20.053663737868536	31.90937247638989	33.32156656044333	14.71539722529824
14	15.196545404430431	34.15420678249684	35.05563426935594	15.593613543716787
15	14.659908025745056	22.78399831719029	47.40071180916551	15.155381847899147
16	17.451413705227832	20.02156704400934	45.26939462371311	17.257624627049715
17	18.131851525892543	19.469092878523384	35.007821680198454	27.39123391538562
18	20.02386398255294	22.68244945526289	37.78584795717539	19.507838605008775
19	23.84875022364928	22.722222759517788	33.696692892063226	19.7323341247697
20	26.168779044183427	22.707050875979824	32.7608113270502	18.36335875278655
21	21.751947562101964	24.055232909568318	35.058414773908716	19.134404754421002
22	21.791237300347685	22.052181608050407	33.5606899519819	22.595891139620015
23	18.408451283142405	25.8958060320024	33.08703704599196	22.60870563886323
24	18.802497134871395	22.64739091959748	40.56979791776477	17.98031402776636
25	17.075380687340726	24.470797448705735	38.27787637151409	20.175945492439446
26	17.616188822855264	29.318788473720602	33.95328510568335	19.11173759774078
27	17.268867536763103	28.72678278698434	35.43789319961122	18.566456476641342
28	15.382295197705965	26.268091413318373	41.031301227774094	17.318312161201565
29	17.12005009743855	22.04831307997698	40.304320178726	20.52731664385847
30	19.08544369599172	25.77352427743149	36.307828450122585	18.833203576454206
31	24.468621401664432	24.74364956938447	33.01685952890999	17.770869500041105
32	24.867502913485207	25.12379289834959	32.234389280308704	17.774314907856496
33	24.732104430915342	25.863830229645497	30.95825858208775	18.445806757351413
34	20.091079657828693	26.1870336610299	33.05705595342292	20.664830727718485
35	20.22726393516347	26.332284801036764	33.18574495761544	20.254706306184325
36	25.768144605579383	25.339644675696455	30.789554490635744	18.102656228088414
37	21.190890099953094	29.191006155795296	31.44714381736679	18.17095992688482
38	19.859753768188128	29.440707553784627	29.90287576705658	20.79666291097066
39	20.831116989124602	28.743828488807864	30.78308679526299	19.641967726804545
40	22.467202135427495	26.641223035150414	29.621379903963792	21.2701949254583
41	19.268231647461036	25.59297281875462	30.098538663520262	25.040256870264077
42	22.025283248789876	26.22765320580086	28.64753840723028	23.099525138178986
43	22.23079880269056	26.385960628055532	28.550039410629747	22.83320115862416
44	20.504045029666777	28.870945903470552	29.721115393356772	20.9038936735059
45	18.398779962958844	32.19878673288297	27.83756534185699	21.564867962301193
46	21.647920424377528	29.711081398666323	29.651723671039715	18.98927450591643
47	21.919382292779876	27.00371620478053	29.768263079251632	21.308638423187958
48	21.991977639907738	25.06661121776428	31.58616904500549	21.355242097322495
49	20.902745204234105	24.74939191574346	33.29388240641789	21.05398047360455
50	19.5271812453759	29.555735818217865	30.353317504605965	20.563765431800267
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	392.0
1	1308.5
2	2225.0
3	20430.5
4	38636.0
5	28382.0
6	18128.0
7	18349.5
8	18571.0
9	18751.0
10	18931.0
11	18658.5
12	18386.0
13	17371.0
14	16356.0
15	15079.0
16	13802.0
17	12725.5
18	11649.0
19	10943.0
20	10237.0
21	10125.0
22	10013.0
23	10316.0
24	10619.0
25	11370.0
26	12121.0
27	14272.5
28	16424.0
29	19672.5
30	22921.0
31	27468.5
32	32016.0
33	37645.0
34	43274.0
35	47974.5
36	52675.0
37	56252.0
38	59829.0
39	74349.5
40	88870.0
41	101341.0
42	113812.0
43	130409.5
44	147007.0
45	149059.5
46	151112.0
47	145338.5
48	139565.0
49	133768.5
50	127972.0
51	118127.0
52	108282.0
53	97599.0
54	86916.0
55	77322.5
56	67729.0
57	61888.5
58	56048.0
59	50846.5
60	45645.0
61	40525.5
62	35406.0
63	28817.0
64	22228.0
65	17937.5
66	13647.0
67	11512.5
68	9378.0
69	7410.5
70	5443.0
71	4548.5
72	3654.0
73	2917.0
74	2180.0
75	1596.5
76	1013.0
77	832.0
78	651.0
79	464.5
80	278.0
81	220.0
82	162.0
83	135.0
84	108.0
85	68.0
86	28.0
87	27.5
88	27.0
89	16.5
90	6.0
91	4.5
92	3.0
93	1.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1654376.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.959778693456794
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.8095052969146	17.74558852647969
2	7.308217468281191	3.209736764942201
3	2.419973339195081	1.594262369183689
4	1.184533098729938	1.0404833881273619
5	0.772820280979303	0.8485481170060295
6	0.5521398861987481	0.7274921825252957
7	0.4419482136621638	0.6793559476192773
8	0.3817594205327793	0.6706681911233712
9	0.2936810859788121	0.5804254489093894
>10	3.7056117520586414	19.485957724706516
>50	1.1552752779304012	18.219913169396406
>100	0.9725824627792132	33.51633103859188
>500	0.0013945833994290346	0.16322987923609128
>1k	2.789166798858069E-4	0.24254796314255966
>5k	0.0	0.0
>10k+	2.789166798858069E-4	1.2754592890102683
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20824	1.2587223218905497	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3960	0.239365174543151	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04448807284438362	0.0
2	0.0	0.0	0.0	0.18810717757027423	0.0
3	0.0	0.0	0.0	0.25211922803522296	0.0
4	0.0	0.0	0.0	0.3355343646184422	0.0
5	0.0	0.0	0.0	0.5288398767873809	0.0
6	0.0	0.0	0.0	0.6883562140650009	0.0
7	0.0	0.0	0.0	0.7989114929133402	0.0
8	0.0	0.0	0.0	1.1013215859030836	0.0
9	0.0	0.0	0.0	1.2527986382781182	0.0
10	0.0	0.0	0.0	1.5856733898460809	0.0
11	0.0	0.0	0.0	1.9453860549234274	0.0
12	6.044575114726036E-5	0.0	0.0	2.253175819765277	0.0
13	6.044575114726036E-5	0.0	0.0	2.3700174567329313	0.0
14	6.044575114726036E-5	0.0	0.0	2.4106974472550378	0.0
15	6.044575114726036E-5	0.0	0.0	2.4794242663094725	0.0
16	6.044575114726036E-5	0.0	0.0	2.6419628911444555	0.0
17	6.044575114726036E-5	0.0	0.0	2.862771220085398	0.0
18	6.044575114726036E-5	0.0	0.0	3.132057041446443	0.0
19	6.044575114726036E-5	0.0	0.0	3.270477811573669	0.0
20	6.044575114726036E-5	0.0	0.0	3.4229219959670596	0.0
21	6.044575114726036E-5	0.0	0.0	3.604198803657693	0.0
22	6.044575114726036E-5	0.0	0.0	3.7987736766007245	0.0
23	6.044575114726036E-5	0.0	0.0	4.005800374280091	0.0
24	6.044575114726036E-5	0.0	0.0	4.167674095852455	0.0
25	6.044575114726036E-5	0.0	0.0	4.304946396707883	0.0
26	6.044575114726036E-5	0.0	0.0	4.433454063646958	0.0
27	6.044575114726036E-5	0.0	0.0	4.569336112225999	0.0
28	6.044575114726036E-5	0.0	0.0	4.708844905873876	0.0
29	6.044575114726036E-5	0.0	0.0	4.858327248461051	0.0
30	6.044575114726036E-5	0.0	0.0	5.046253088777884	0.0
31	6.044575114726036E-5	6.044575114726036E-5	0.0	5.216589215510863	0.0
32	6.044575114726036E-5	6.044575114726036E-5	0.0	5.377133130557987	0.0
33	6.044575114726036E-5	6.044575114726036E-5	0.0	5.536165901826429	0.0
34	6.044575114726036E-5	6.044575114726036E-5	0.0	5.705474450789905	0.0
35	6.044575114726036E-5	6.044575114726036E-5	0.0	5.902044033520796	0.0
36	6.044575114726036E-5	6.044575114726036E-5	0.0	6.078847855626532	0.0
37	6.044575114726036E-5	6.044575114726036E-5	0.0	6.263328288127971	0.0
38	6.044575114726036E-5	6.044575114726036E-5	0.0	6.447264708869084	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACGA	45	4.802132E-10	44.0	8
CGAACGT	20	7.8574865E-4	44.0	15
CGTGCTA	20	7.8574865E-4	44.0	18
ACCGCGT	20	7.8574865E-4	44.0	23
CGACCGT	20	7.8574865E-4	44.0	19
CGCGAGA	20	7.8574865E-4	44.0	20
CGACAGG	140	0.0	44.0	2
CCGCTAG	20	7.8574865E-4	44.0	32
CCGTAGT	25	4.443691E-5	44.0	41
TAACCGA	25	4.443691E-5	44.0	39
CTACGAC	20	7.8574865E-4	44.0	13
TACGACA	30	2.5283953E-6	44.0	28
GCGATCG	30	2.5283953E-6	44.0	9
TTACGCG	30	2.5283953E-6	44.0	1
ATACGAC	30	2.5283953E-6	44.0	27
CGTCCAT	20	7.8574865E-4	44.0	15
CGTCACG	20	7.8574865E-4	44.0	21
TATCGAT	25	4.443691E-5	44.0	29
TAATGCG	45	4.802132E-10	44.0	1
AATACGA	20	7.8574865E-4	44.0	21
>>END_MODULE