FastQCFastQC Report
Thu 26 May 2016
SRR1545881_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545881_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences907158
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81100.894000824553165No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA14590.1608319609152981No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAACT207.855603E-444.033
CCGTGTA207.855603E-444.019
TAACGGC254.442095E-544.019
ATGTACG207.855603E-444.01
ATGCGAT207.855603E-444.019
CGGTCTA1400.044.031
CACGTTA207.855603E-444.032
CGCAATA207.855603E-444.043
TAGTGCG600.044.01
TTCGAAC207.855603E-444.033
CCGTTTA254.442095E-544.038
GCGTATG207.855603E-444.01
AATACGG502.7284841E-1144.02
TGCGTAG207.855603E-444.01
CGAAGTA408.309144E-944.023
CGTTTTT53750.042.485581
AGTACGG750.041.0666662
CTCACGA1450.040.9655224
TGATTCG700.040.85714315
GGATCGA557.8216544E-1140.0000048
Produced by FastQC (version 0.11.5)