##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545881_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	907158
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.49695642875883	31.0	31.0	33.0	30.0	34.0
2	31.91185879416816	31.0	31.0	34.0	30.0	34.0
3	32.00553376589304	33.0	31.0	34.0	30.0	34.0
4	35.75999329774967	37.0	35.0	37.0	35.0	37.0
5	35.6870710504675	37.0	35.0	37.0	33.0	37.0
6	35.73132243776718	37.0	35.0	37.0	33.0	37.0
7	35.96730889216652	37.0	35.0	37.0	35.0	37.0
8	35.95182537110404	37.0	35.0	37.0	35.0	37.0
9	37.73757823885145	39.0	38.0	39.0	35.0	39.0
10	37.28465824035063	39.0	37.0	39.0	34.0	39.0
11	37.01859764230707	39.0	37.0	39.0	33.0	39.0
12	36.41842655854879	38.0	35.0	39.0	32.0	39.0
13	36.173890325610316	38.0	35.0	39.0	32.0	39.0
14	37.23494584184894	39.0	35.0	41.0	32.0	41.0
15	37.37797825737082	39.0	35.0	41.0	32.0	41.0
16	37.499959213279276	39.0	35.0	41.0	33.0	41.0
17	37.42564911514863	39.0	35.0	41.0	33.0	41.0
18	37.307187943004415	39.0	35.0	41.0	32.0	41.0
19	37.184719751134864	38.0	35.0	41.0	32.0	41.0
20	37.0098384184453	38.0	35.0	41.0	32.0	41.0
21	36.890464505631876	38.0	35.0	41.0	32.0	41.0
22	36.775641068038865	38.0	35.0	41.0	32.0	41.0
23	36.68910928415998	38.0	35.0	41.0	31.0	41.0
24	36.73340256052418	38.0	35.0	41.0	32.0	41.0
25	36.715058457291896	38.0	35.0	41.0	32.0	41.0
26	36.482018567879024	38.0	35.0	41.0	31.0	41.0
27	36.429378344235516	38.0	35.0	41.0	31.0	41.0
28	36.414533080235195	38.0	35.0	40.0	31.0	41.0
29	36.37780298470608	38.0	35.0	40.0	31.0	41.0
30	36.28887580774242	38.0	35.0	40.0	31.0	41.0
31	36.12977342425465	38.0	35.0	40.0	30.0	41.0
32	35.930823516961766	38.0	35.0	40.0	30.0	41.0
33	35.69664600874379	38.0	35.0	40.0	29.0	41.0
34	35.450251224152794	38.0	35.0	40.0	27.0	41.0
35	35.22871539467215	38.0	34.0	40.0	26.0	41.0
36	35.06550788286054	38.0	34.0	40.0	25.0	41.0
37	34.96195701300104	38.0	34.0	40.0	25.0	41.0
38	34.89841681382956	38.0	34.0	40.0	25.0	41.0
39	34.79759314253967	37.0	34.0	40.0	24.0	41.0
40	34.65735516855939	37.0	34.0	40.0	23.0	41.0
41	34.619163365146974	37.0	34.0	40.0	23.0	41.0
42	34.59380284360608	37.0	34.0	40.0	23.0	41.0
43	34.50058093518439	37.0	34.0	40.0	23.0	41.0
44	34.35804236968643	37.0	34.0	40.0	23.0	41.0
45	34.28446202315363	36.0	34.0	40.0	23.0	41.0
46	34.21824974260272	36.0	33.0	40.0	23.0	41.0
47	34.162444689899665	36.0	33.0	40.0	23.0	41.0
48	34.033253303173204	36.0	33.0	40.0	23.0	41.0
49	33.96737833982614	36.0	33.0	40.0	23.0	41.0
50	33.80832225477811	35.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	3.0
13	2.0
14	13.0
15	30.0
16	73.0
17	150.0
18	362.0
19	695.0
20	1254.0
21	2064.0
22	3192.0
23	4676.0
24	6766.0
25	10256.0
26	14764.0
27	18870.0
28	20193.0
29	20760.0
30	22472.0
31	26322.0
32	32216.0
33	41609.0
34	72956.0
35	115913.0
36	62939.0
37	81200.0
38	125572.0
39	221833.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.670227237151632	22.688660630232	29.471492286900407	16.16961984571596
2	33.00549628620373	25.48784225019236	29.3123138416902	12.194347621913714
3	27.45078586089744	24.86468729813329	34.61690245800621	13.067624382963055
4	25.858891174415042	27.254899367034184	33.09037675906513	13.795832699485647
5	22.845634387835414	31.905908342317435	32.00015873750769	13.248298532339462
6	21.5278926052573	39.37406714155638	28.79310991029126	10.304930342895064
7	85.91700673973001	3.7792754955586565	8.730452688506302	1.573265076205027
8	87.5231216612762	2.5378159041754578	8.426646736290701	1.5124156982576353
9	82.3112401588257	5.420775653193821	9.77591555164591	2.4920686363345745
10	43.57730406390067	31.565284107068447	14.513899453017004	10.34351237601388
11	39.17884205397516	19.654128608246854	25.598738036813874	15.568291300964109
12	35.72575008984102	19.512146726369608	28.356802232907608	16.405300950881767
13	22.419909210964352	30.93738907665478	28.85880960097359	17.783892111407273
14	17.02062926193673	32.85326260695491	32.11325921173599	18.01284891937237
15	17.00376340174479	22.17386607404664	43.8488113426768	16.973559181531773
16	19.325078982933512	19.29597710652389	40.71705259723223	20.66189131331036
17	19.349661249749218	19.817385725529622	31.427270662883423	29.405682361837737
18	22.40976764797312	22.487372651732112	33.43276474439954	21.670094955895223
19	26.554800817498165	22.78114727533682	28.946886870864834	21.71716503630018
20	28.260126681349885	22.419798976584012	28.73589826689507	20.58417607517103
21	24.222351563895153	25.141596061546057	29.938665590779113	20.697386783779674
22	24.055015774539825	23.028072287297253	28.775251940676267	24.141659997486656
23	21.656756595874146	25.260649192312695	29.51459392961314	23.568000282200014
24	20.981350547534166	22.407011788464633	37.11679773534489	19.49483992865631
25	19.700537282369773	24.361687820644253	34.15347712305905	21.784297773926923
26	19.694584625831443	28.75783490858263	29.52837322715558	22.01920723843035
27	19.291677965690653	28.07625573494364	31.631424735272134	21.000641564093574
28	17.815970316086062	26.168539548788633	36.581940521937746	19.43354961318756
29	19.1502472557151	22.63773234651516	36.20416730051436	22.007853097255385
30	20.68746568954912	25.801459062258175	32.43955297754085	21.07152227065186
31	26.55281659865205	24.98098456939144	28.307417230515526	20.158781601440985
32	27.893707601101465	24.704186040359012	28.380723093441272	19.02138326509825
33	26.41436221694567	24.89676550281208	28.059941046653396	20.628931233588858
34	20.982122188196545	26.113201889858217	30.773580787470316	22.131095134474922
35	22.102875133108014	24.890151439991712	31.541693949675803	21.465279477224474
36	26.704388871618832	25.771254842045156	27.641601573265078	19.882754713070934
37	22.776186728221546	29.59164776147044	28.082759563383668	19.54940594692435
38	22.628252189805963	29.294125168934187	26.588532537882042	21.4890901033778
39	23.212935343126556	27.691317278798184	27.823157597684194	21.27258978039107
40	23.943348347255935	25.726168980486307	26.727868794631142	23.60261387762661
41	19.689954781857185	25.309703491563763	28.276661838400805	26.723679888178243
42	21.769305898200756	25.610533115510197	26.60142996038176	26.018731025907282
43	22.630346643032414	25.758577888306117	27.364803044232648	24.24627242442882
44	21.18153618223066	27.63862524499591	28.433415127243546	22.74642344552989
45	19.507516882395347	31.68279395651033	26.395512137907616	22.4141770231867
46	21.633497141622517	30.029168017037826	27.712041342302	20.625293499037653
47	22.06418286560886	25.527085689593214	29.79040034922252	22.61833109557541
48	22.634645783865658	23.560945281858288	31.420325896922037	22.38408303735402
49	21.479940649809627	24.33335758489701	31.875924590865097	22.31077717442827
50	20.073349956677887	28.933768979604434	29.637946201213015	21.354934862504656
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	249.0
1	799.0
2	1349.0
3	8329.0
4	15309.0
5	10849.5
6	6390.0
7	6380.0
8	6370.0
9	6279.5
10	6189.0
11	6113.5
12	6038.0
13	5632.0
14	5226.0
15	4837.5
16	4449.0
17	4012.5
18	3576.0
19	3298.5
20	3021.0
21	2938.0
22	2855.0
23	3076.5
24	3298.0
25	4645.5
26	5993.0
27	7748.5
28	9504.0
29	11112.0
30	12720.0
31	14671.0
32	16622.0
33	19501.0
34	22380.0
35	23812.0
36	25244.0
37	26922.5
38	28601.0
39	33992.0
40	39383.0
41	47740.5
42	56098.0
43	64066.0
44	72034.0
45	73699.5
46	75365.0
47	72632.5
48	69900.0
49	68545.5
50	67191.0
51	62688.5
52	58186.0
53	55633.0
54	53080.0
55	49794.5
56	46509.0
57	43513.5
58	40518.0
59	39634.0
60	38750.0
61	36565.5
62	34381.0
63	29265.0
64	24149.0
65	20482.0
66	16815.0
67	13811.0
68	10807.0
69	9227.5
70	7648.0
71	6282.5
72	4917.0
73	3752.0
74	2587.0
75	2142.0
76	1697.0
77	1246.5
78	796.0
79	620.5
80	445.0
81	350.0
82	255.0
83	188.5
84	122.0
85	92.0
86	62.0
87	44.5
88	27.0
89	19.0
90	11.0
91	11.0
92	11.0
93	6.5
94	2.0
95	15.0
96	28.0
97	14.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	907158.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.24535544408976
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.94120264527442	15.000061486481354
2	5.893102956882035	2.268297221476223
3	2.1796745857571658	1.2584583646603733
4	1.2246295959360423	0.9427372744456464
5	0.9423540226072891	0.9067969059622538
6	0.7149495960597234	0.8255675460466664
7	0.5892025079921707	0.7937588186400927
8	0.5119866647725649	0.7882692276945635
9	0.46839218851114744	0.8112936739618916
>10	6.7445736808480135	31.335988318726894
>50	2.230092623209068	30.48981839308945
>100	0.5569442117152448	13.287930997339092
>500	0.001736832261066252	0.22484525199253977
>1k	5.78944087022084E-4	0.16256155731274308
>5k	5.78944087022084E-4	0.9036149621702168
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8110	0.894000824553165	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1459	0.1608319609152981	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.024141329294345638	0.0
2	0.0	0.0	0.0	0.07870734756238715	0.0
3	0.0	0.0	0.0	0.1106753178608357	0.0
4	0.0	0.0	0.0	0.17990250871402777	0.0
5	0.0	0.0	0.0	0.31813642165973294	0.0
6	0.0	0.0	0.0	0.44170916202028754	0.0
7	0.0	0.0	0.0	0.5347469790268068	0.0
8	0.0	0.0	0.0	0.7767114438719606	0.0
9	0.0	0.0	0.0	0.8822057458568409	0.0
10	0.0	0.0	0.0	1.0661869266434292	0.0
11	0.0	0.0	0.0	1.2261370125160116	0.0
12	0.0	0.0	0.0	1.3844335826835017	0.0
13	0.0	0.0	0.0	1.4410940541779933	0.0
14	0.0	0.0	0.0	1.4572985080878964	0.0
15	0.0	0.0	0.0	1.485518509454803	0.0
16	0.0	0.0	0.0	1.5626825756924372	0.0
17	0.0	0.0	0.0	1.6562715646006538	0.0
18	0.0	0.0	0.0	1.7866788365422561	0.0
19	0.0	0.0	0.0	1.854032042929677	0.0
20	0.0	0.0	0.0	1.932188218590367	0.0
21	0.0	0.0	0.0	2.0252260355968863	0.0
22	0.0	0.0	0.0	2.1249881498041137	0.0
23	0.0	0.0	0.0	2.2299312798872966	0.0
24	0.0	0.0	0.0	2.3160243309324287	0.0
25	0.0	0.0	0.0	2.385582224926639	0.0
26	0.0	0.0	0.0	2.4555810564422074	0.0
27	0.0	0.0	0.0	2.5250287160560783	0.0
28	0.0	0.0	0.0	2.5938149693879127	0.0
29	0.0	0.0	0.0	2.66612872289061	0.0
30	0.0	0.0	0.0	2.7686466966063246	0.0
31	0.0	0.0	0.0	2.8635584980786146	0.0
32	0.0	0.0	0.0	2.948108267798994	0.0
33	0.0	0.0	0.0	3.0360753033098975	0.0
34	0.0	0.0	0.0	3.1334122611496564	0.0
35	0.0	0.0	0.0	3.2450796884335475	0.0
36	0.0	0.0	0.0	3.3461646152048488	0.0
37	0.0	0.0	0.0	3.4493439952025997	0.0
38	0.0	0.0	0.0	3.552082437678993	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAACT	20	7.855603E-4	44.0	33
CCGTGTA	20	7.855603E-4	44.0	19
TAACGGC	25	4.442095E-5	44.0	19
ATGTACG	20	7.855603E-4	44.0	1
ATGCGAT	20	7.855603E-4	44.0	19
CGGTCTA	140	0.0	44.0	31
CACGTTA	20	7.855603E-4	44.0	32
CGCAATA	20	7.855603E-4	44.0	43
TAGTGCG	60	0.0	44.0	1
TTCGAAC	20	7.855603E-4	44.0	33
CCGTTTA	25	4.442095E-5	44.0	38
GCGTATG	20	7.855603E-4	44.0	1
AATACGG	50	2.7284841E-11	44.0	2
TGCGTAG	20	7.855603E-4	44.0	1
CGAAGTA	40	8.309144E-9	44.0	23
CGTTTTT	5375	0.0	42.48558	1
AGTACGG	75	0.0	41.066666	2
CTCACGA	145	0.0	40.96552	24
TGATTCG	70	0.0	40.857143	15
GGATCGA	55	7.8216544E-11	40.000004	8
>>END_MODULE