FastQCFastQC Report
Thu 26 May 2016
SRR1545880_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545880_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2749580
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT399701.4536765615112126No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA88250.3209581099658857No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCC31460.1144174746688585No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTATCG207.8583957E-444.00000438
GTATCGC207.8583957E-444.00000439
CCGCGTA207.8583957E-444.00000418
AGCGTAC551.8189894E-1244.034
CGTATAT254.4444627E-544.034
CGTTGCG254.4444627E-544.026
CCGTACA302.5290083E-644.013
TCTACCG502.7284841E-1144.01
TATTCGC302.5290083E-644.017
GTCGTAA254.4444627E-544.030
CGTACGA551.8189894E-1244.036
CGCCCGT302.5290083E-644.023
AGTCGAC254.4444627E-544.044
CGTTTTT289800.043.316771
ATTAGCG2400.042.1666641
TAACGCG1000.041.8000031
TATAGCG850.041.4117661
CGATCTA800.041.25000410
TATTACG603.6379788E-1240.3333321
TACTACG603.6379788E-1240.33333227
Produced by FastQC (version 0.11.5)