##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545880_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2749580
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.53239549312986	31.0	31.0	33.0	30.0	34.0
2	31.933578219218937	31.0	31.0	34.0	30.0	34.0
3	31.92151383120331	31.0	31.0	34.0	30.0	34.0
4	35.70234290327977	37.0	35.0	37.0	33.0	37.0
5	35.646863157282205	37.0	35.0	37.0	33.0	37.0
6	35.69768691945679	37.0	35.0	37.0	33.0	37.0
7	36.01566566530161	37.0	35.0	37.0	35.0	37.0
8	36.0091337586104	37.0	35.0	37.0	35.0	37.0
9	37.795218178776395	39.0	38.0	39.0	35.0	39.0
10	37.33462346976629	39.0	37.0	39.0	34.0	39.0
11	37.049834883873174	39.0	37.0	39.0	33.0	39.0
12	36.36098858734788	38.0	35.0	39.0	32.0	39.0
13	36.043385535245385	38.0	35.0	39.0	32.0	39.0
14	37.10912102939358	39.0	35.0	41.0	32.0	41.0
15	37.2734275780301	39.0	35.0	41.0	32.0	41.0
16	37.41219713556252	39.0	35.0	41.0	33.0	41.0
17	37.339881363699185	39.0	35.0	41.0	32.0	41.0
18	37.16854646891525	39.0	35.0	40.0	32.0	41.0
19	36.98904669076732	38.0	35.0	40.0	32.0	41.0
20	36.717416114461116	38.0	35.0	40.0	32.0	41.0
21	36.60125691923857	38.0	35.0	40.0	32.0	41.0
22	36.493552469831755	37.0	35.0	40.0	32.0	41.0
23	36.42250852857527	37.0	35.0	40.0	31.0	41.0
24	36.438469148015336	37.0	35.0	40.0	32.0	41.0
25	36.419667367379745	37.0	35.0	40.0	32.0	41.0
26	36.17678809127212	37.0	35.0	40.0	31.0	41.0
27	36.16255100779028	37.0	35.0	40.0	31.0	41.0
28	36.16723426850646	37.0	35.0	40.0	31.0	41.0
29	36.12630474472465	37.0	35.0	40.0	31.0	41.0
30	35.98447581085111	37.0	35.0	40.0	31.0	41.0
31	35.74470937379527	37.0	35.0	40.0	30.0	41.0
32	35.395560412863055	37.0	34.0	40.0	29.0	41.0
33	34.99732904661803	37.0	34.0	40.0	25.0	41.0
34	34.62122978782214	37.0	34.0	40.0	23.0	41.0
35	34.27912808501662	37.0	34.0	40.0	22.0	41.0
36	34.07197499254431	36.0	33.0	40.0	20.0	41.0
37	33.97950377875894	36.0	33.0	40.0	20.0	41.0
38	33.89082550789575	36.0	33.0	40.0	19.0	41.0
39	33.799844339862815	36.0	33.0	40.0	18.0	41.0
40	33.67258890448723	36.0	33.0	40.0	18.0	41.0
41	33.63382043803053	36.0	33.0	40.0	18.0	41.0
42	33.5773223546869	36.0	33.0	40.0	18.0	41.0
43	33.468667942012964	36.0	33.0	40.0	18.0	41.0
44	33.32152074134959	35.0	33.0	40.0	17.0	41.0
45	33.23017442663971	35.0	33.0	40.0	17.0	41.0
46	33.1835065719128	35.0	33.0	40.0	17.0	41.0
47	33.14428821856429	35.0	33.0	40.0	17.0	41.0
48	33.04730540664392	35.0	33.0	40.0	17.0	41.0
49	32.9860302300715	35.0	32.0	40.0	15.0	41.0
50	32.82368070759898	35.0	32.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	8.0
13	5.0
14	24.0
15	58.0
16	152.0
17	462.0
18	1138.0
19	2556.0
20	4239.0
21	7314.0
22	11460.0
23	17391.0
24	26309.0
25	41883.0
26	63399.0
27	78500.0
28	78641.0
29	73734.0
30	75058.0
31	84070.0
32	99942.0
33	127165.0
34	223721.0
35	378057.0
36	174228.0
37	213386.0
38	334507.0
39	632170.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.3585202103594	22.01278013369315	28.94805752151238	19.68064213443508
2	34.35626532052168	24.31655016402505	29.19154925479528	12.135635260657992
3	25.698506681020373	23.74966358498389	38.08628954240284	12.465540191592899
4	22.860036805621224	25.638606623557052	37.78558907178551	13.715767499036216
5	22.396075036914727	30.03458710057536	35.0566995686614	12.512638293848516
6	19.796405269168382	38.12291331767034	32.10988587347886	9.970795539682424
7	80.85220288189468	3.796943533194161	13.799380268986535	1.551473315924614
8	82.23506862866329	2.996966809476356	13.128950603364878	1.6390139584954795
9	77.39167436481208	5.398206271503284	14.551713352584757	2.6584060110998773
10	41.22295041424508	31.975174390270517	18.408775158387826	8.393100037096575
11	39.86783436015682	19.493668123858917	28.798143716494884	11.840353799489376
12	35.79452134507816	19.722757657533148	31.2453902050495	13.237330792339193
13	20.85482146364172	33.25369692825814	31.963027080499568	13.928454527600579
14	15.51135082448956	35.957382582067076	33.380370820270734	15.150895773172628
15	14.622051367845271	22.647858945729894	48.70638424777602	14.023705438648811
16	16.38031990340343	19.46828242858909	46.95091613991955	17.200481528087927
17	16.760086995104707	18.629827100866315	35.03160482691902	29.57848107710996
18	20.17431753213218	21.6088638992137	38.97624364448388	19.240574924170236
19	25.46788964132704	22.756821041759103	32.52482197281039	19.250467344103463
20	27.768059121756778	21.76197819303312	32.229067712159676	18.240894973050427
21	21.41410688177831	25.085940398169903	33.90397806210403	19.59597465794776
22	21.32987583558216	21.937895969566263	32.84410709999345	23.88812109485812
23	18.260752551298744	26.310018257333844	32.571592752347634	22.857636439019778
24	18.858843896158685	21.876031975792667	42.23288647720743	17.03223765084122
25	16.565366346860248	23.758864990289428	39.33837167858364	20.337396984266686
26	16.72600906320238	29.760945308010676	33.52075589726431	19.992289731522632
27	16.643341892216267	29.53534721666582	35.38409502542206	18.43721586569585
28	14.532255835436686	26.006699204969486	41.70135075175118	17.759694207842653
29	15.987096211057688	22.38498243368078	41.227969362593555	20.399951992667972
30	17.792899279162636	27.13643538285847	36.38152008670415	18.68914525127474
31	25.345907374944538	24.601248190632752	31.205311356643563	18.84753307777915
32	26.04361393376443	24.86990740403989	32.15185591981321	16.934622742382473
33	24.22911862902698	27.218920707889932	30.39242357014526	18.159537092937832
34	18.816728373060613	26.41657998676161	32.849598847824026	21.91709279235374
35	18.98820910830018	26.825806123116987	33.24835065719128	20.93763411139156
36	25.475963601713715	25.773281737574465	30.68395900464798	18.066795656063835
37	19.341972228485805	30.364019232028166	31.769288400410247	18.524720139075786
38	20.000618276245827	30.52633493115312	28.125568268608298	21.347478523992756
39	20.252947722924954	29.136122607816468	30.560049171146137	20.050880498112438
40	22.738927399821062	25.932360578706565	29.27083409102481	22.05787793044756
41	18.056394067457575	25.299754871653125	30.865041206293327	25.778809854595973
42	20.791466333039953	25.815142676336023	28.259952429098263	25.13343856152576
43	21.518231875413697	26.285032623164266	28.88993955440467	23.30679594701736
44	19.719993599022395	29.12215683849897	29.52505473563235	21.632794826846283
45	17.575775209304695	34.26257828468348	26.44669367685247	21.714952829159362
46	21.41148830003128	30.188646993358986	28.952967362288057	19.446897344321677
47	20.565868241695096	27.28609460353945	30.007601160904574	22.14043599386088
48	21.87057659715302	23.973988754646165	32.28405792884732	21.871376719353503
49	19.70977385637079	24.42274820154351	33.55188792470123	22.315590017384473
50	18.547050822307405	30.210613984681295	30.287534823500316	20.954800369510977
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	947.0
1	2395.5
2	3844.0
3	38192.5
4	72541.0
5	52183.0
6	31825.0
7	31713.0
8	31601.0
9	32247.0
10	32893.0
11	31685.5
12	30478.0
13	28678.0
14	26878.0
15	24708.5
16	22539.0
17	21116.0
18	19693.0
19	18426.0
20	17159.0
21	15796.5
22	14434.0
23	15950.5
24	17467.0
25	17948.5
26	18430.0
27	21265.0
28	24100.0
29	31452.5
30	38805.0
31	44578.0
32	50351.0
33	57937.0
34	65523.0
35	73772.0
36	82021.0
37	88707.5
38	95394.0
39	113046.5
40	130699.0
41	165641.0
42	200583.0
43	237164.0
44	273745.0
45	275781.0
46	277817.0
47	250903.0
48	223989.0
49	211099.0
50	198209.0
51	177537.0
52	156865.0
53	146444.5
54	136024.0
55	121817.0
56	107610.0
57	99348.0
58	91086.0
59	87609.0
60	84132.0
61	75918.0
62	67704.0
63	54268.0
64	40832.0
65	33052.0
66	25272.0
67	20109.0
68	14946.0
69	12491.0
70	10036.0
71	8431.0
72	6826.0
73	4922.5
74	3019.0
75	2201.0
76	1383.0
77	1145.0
78	907.0
79	749.5
80	592.0
81	400.5
82	209.0
83	154.0
84	99.0
85	67.0
86	35.0
87	23.5
88	12.0
89	9.0
90	6.0
91	11.5
92	17.0
93	9.0
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2749580.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.29917341604918
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.52964349526874	14.736259114525396
2	8.322894309617812	3.046041725902905
3	2.939139091210035	1.6135144777182486
4	1.390050859818483	1.0174712696378676
5	0.8308309385550389	0.7601759712018779
6	0.537989856603569	0.5906861809238485
7	0.36645622203543915	0.46940921694917076
8	0.2763231284794535	0.4045187877528606
9	0.22703729749110052	0.3739135390830717
>10	2.2948245747736	9.666673494218383
>50	0.8679372322365417	11.572485719436902
>100	1.4001374802106814	51.76861095156552
>500	0.015122452138082188	1.7772234130287075
>1k	0.001209796171046575	0.40262133583094767
>5k	2.0163269517442918E-4	0.32561705358395465
>10k+	2.0163269517442918E-4	1.4747777486403022
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	39970	1.4536765615112126	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	8825	0.3209581099658857	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCC	3146	0.1144174746688585	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05819070548956568	0.0
2	0.0	0.0	0.0	0.22247034092479578	0.0
3	0.0	0.0	0.0	0.3071378174121138	0.0
4	0.0	0.0	0.0	0.4570152532386765	0.0
5	0.0	0.0	0.0	0.7956124208060867	0.0
6	0.0	0.0	0.0	1.039467845998298	0.0
7	0.0	0.0	0.0	1.2214592774169146	0.0
8	0.0	0.0	0.0	1.664872453247405	0.0
9	0.0	0.0	0.0	1.8525011092603234	0.0
10	0.0	0.0	0.0	2.2907862291695458	0.0
11	0.0	0.0	0.0	2.5731202583667323	0.0
12	0.0	0.0	0.0	2.892441754740724	0.0
13	0.0	0.0	0.0	3.0019857578248312	0.0
14	0.0	0.0	0.0	3.041591806748667	0.0
15	0.0	0.0	0.0	3.1200037823958566	0.0
16	0.0	0.0	0.0	3.2700266949861434	0.0
17	0.0	0.0	0.0	3.459182857018163	0.0
18	0.0	0.0	0.0	3.7459902966998597	0.0
19	0.0	0.0	0.0	3.8717185897482524	0.0
20	0.0	0.0	0.0	4.009921515285971	0.0
21	0.0	0.0	0.0	4.167363742826177	0.0
22	0.0	0.0	0.0	4.343717949650492	0.0
23	0.0	0.0	0.0	4.540948072069189	0.0
24	0.0	0.0	0.0	4.677696229969668	0.0
25	0.0	0.0	0.0	4.795496039395108	0.0
26	0.0	0.0	0.0	4.918896704223918	0.0
27	0.0	0.0	0.0	5.038369496432183	0.0
28	0.0	0.0	0.0	5.157187643203689	0.0
29	0.0	0.0	0.0	5.3024825609729485	0.0
30	0.0	0.0	0.0	5.480837073298467	0.0
31	0.0	0.0	0.0	5.6522450701561695	0.0
32	0.0	0.0	0.0	5.799867616145011	0.0
33	0.0	0.0	0.0	5.948253915143404	0.0
34	0.0	0.0	0.0	6.098604150452069	0.0
35	0.0	0.0	0.0	6.297143563744281	0.0
36	0.0	0.0	0.0	6.467569592446846	0.0
37	0.0	0.0	0.0	6.6386866357771	0.0
38	3.636919093097855E-5	0.0	0.0	6.8096218331527	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTATCG	20	7.8583957E-4	44.000004	38
GTATCGC	20	7.8583957E-4	44.000004	39
CCGCGTA	20	7.8583957E-4	44.000004	18
AGCGTAC	55	1.8189894E-12	44.0	34
CGTATAT	25	4.4444627E-5	44.0	34
CGTTGCG	25	4.4444627E-5	44.0	26
CCGTACA	30	2.5290083E-6	44.0	13
TCTACCG	50	2.7284841E-11	44.0	1
TATTCGC	30	2.5290083E-6	44.0	17
GTCGTAA	25	4.4444627E-5	44.0	30
CGTACGA	55	1.8189894E-12	44.0	36
CGCCCGT	30	2.5290083E-6	44.0	23
AGTCGAC	25	4.4444627E-5	44.0	44
CGTTTTT	28980	0.0	43.31677	1
ATTAGCG	240	0.0	42.166664	1
TAACGCG	100	0.0	41.800003	1
TATAGCG	85	0.0	41.411766	1
CGATCTA	80	0.0	41.250004	10
TATTACG	60	3.6379788E-12	40.333332	1
TACTACG	60	3.6379788E-12	40.333332	27
>>END_MODULE