FastQCFastQC Report
Thu 26 May 2016
SRR1545879_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545879_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2026456
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT450022.220724259495395No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTAA207.8579044E-444.00000427
GGCGCGT207.8579044E-444.00000431
CGTTTTT329000.043.5586621
CGACGGT1900.042.84210628
CGTTAGG2550.041.4117662
CGTTTCG603.6379788E-1240.33333242
TCGTTGA900.039.1111124
GCGATAT2250.039.111119
GTTTTTT373900.038.8456842
GACCGAT8200.038.6341489
CACGACG2050.038.63414826
GCCGATC404.126614E-738.5000049
TAACGGG5150.038.44663
TGGGCGA8650.038.1502886
AGGGCGA14850.037.9259266
CGGTCTA2100.037.71428731
TCGCAAG700.037.7142831
GGACCGA9050.037.6795588
ACGGGAC7750.037.4709665
CCGCAAT651.0913936E-1137.2307733
Produced by FastQC (version 0.11.5)