##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545879_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2026456
Sequences flagged as poor quality	0
Sequence length	50
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.463548184613927	31.0	31.0	33.0	30.0	34.0
2	31.849142048976145	31.0	31.0	34.0	30.0	34.0
3	31.710036141914753	31.0	31.0	34.0	30.0	34.0
4	35.58358533321227	37.0	35.0	37.0	33.0	37.0
5	35.564508679191654	37.0	35.0	37.0	33.0	37.0
6	35.62687223408749	37.0	35.0	37.0	33.0	37.0
7	36.002969223116615	37.0	35.0	37.0	35.0	37.0
8	36.00382441069532	37.0	35.0	37.0	35.0	37.0
9	37.77006162482679	39.0	38.0	39.0	35.0	39.0
10	37.23863977308167	39.0	37.0	39.0	33.0	39.0
11	37.00468305258047	39.0	37.0	39.0	33.0	39.0
12	36.77941193887259	39.0	35.0	39.0	33.0	39.0
13	36.652704524549264	39.0	35.0	39.0	32.0	39.0
14	37.82569865814999	40.0	36.0	41.0	33.0	41.0
15	37.88021649618842	40.0	36.0	41.0	33.0	41.0
16	37.93972284619059	40.0	36.0	41.0	33.0	41.0
17	37.83344666748254	40.0	36.0	41.0	33.0	41.0
18	37.55125450540253	39.0	36.0	41.0	33.0	41.0
19	37.25934143154354	38.0	36.0	41.0	32.0	41.0
20	36.93706993884891	38.0	35.0	41.0	32.0	41.0
21	36.81869431164555	38.0	35.0	41.0	32.0	41.0
22	36.7275805642955	38.0	35.0	40.0	32.0	41.0
23	36.60505779548137	38.0	35.0	40.0	31.0	41.0
24	36.62044031550648	38.0	35.0	40.0	32.0	41.0
25	36.59241503393116	38.0	35.0	40.0	32.0	41.0
26	36.36430250644475	38.0	35.0	40.0	31.0	41.0
27	36.339885988148765	38.0	35.0	40.0	31.0	41.0
28	36.29268634502797	38.0	35.0	40.0	31.0	41.0
29	36.20488873185502	38.0	35.0	40.0	31.0	41.0
30	36.001801174069406	38.0	35.0	40.0	30.0	41.0
31	35.718485868925846	38.0	35.0	40.0	29.0	41.0
32	35.27326574078095	38.0	34.0	40.0	25.0	41.0
33	34.69579995815354	38.0	34.0	40.0	22.0	41.0
34	34.122717690391504	38.0	33.0	40.0	18.0	41.0
35	33.6343730137738	38.0	33.0	40.0	15.0	41.0
36	33.376637341249946	38.0	33.0	40.0	15.0	41.0
37	33.24855017824221	38.0	33.0	40.0	12.0	41.0
38	33.110719403727494	38.0	32.0	40.0	10.0	41.0
39	32.91027044258548	37.0	32.0	40.0	10.0	41.0
40	32.82950086258966	37.0	31.0	40.0	10.0	41.0
41	32.72193820147094	37.0	31.0	40.0	10.0	41.0
42	32.66751560359564	37.0	31.0	40.0	10.0	41.0
43	32.57777617673416	37.0	31.0	40.0	10.0	41.0
44	32.458678599486	37.0	31.0	40.0	10.0	41.0
45	32.36953133944186	37.0	31.0	40.0	10.0	41.0
46	32.29491832045699	37.0	31.0	40.0	10.0	41.0
47	32.23522346401797	36.0	31.0	40.0	10.0	41.0
48	32.07372427528651	36.0	30.0	40.0	10.0	41.0
49	31.981898940810954	36.0	30.0	40.0	10.0	41.0
50	31.849432704188988	36.0	30.0	40.0	9.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	9.0
14	21.0
15	50.0
16	142.0
17	455.0
18	1089.0
19	2279.0
20	4190.0
21	6747.0
22	10208.0
23	15825.0
24	24620.0
25	40614.0
26	62895.0
27	74939.0
28	70585.0
29	61511.0
30	58584.0
31	61138.0
32	69326.0
33	84004.0
34	123908.0
35	180234.0
36	137611.0
37	174871.0
38	273160.0
39	487437.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.020001421200362	21.610831915422786	26.910280805504783	23.45888585787207
2	39.95453145787523	22.39643989309415	26.410294622730518	11.238734026300103
3	24.388439719391883	21.643253048672165	42.11441057688891	11.853896655047038
4	21.90020410016304	24.53317515899679	41.28374857386491	12.282872166975253
5	20.31605916930839	28.271326887926506	39.713371521513416	11.699242421251682
6	18.19156201763078	35.90736734476347	36.58436205868768	9.316708578918071
7	76.74511561070165	2.8352453741902117	18.96976791008539	1.4498711050227588
8	77.60454705160141	2.216825827947905	18.592113522326663	1.5865135981240155
9	72.3662393854098	5.366955907258781	20.094391390684034	2.1724133166473885
10	28.843113297303276	37.97822405223701	24.457032375733792	8.721630274725925
11	25.632483508154134	20.09473682132748	39.70340338008819	14.569376290430188
12	25.11981508604184	20.219091852968926	39.1079796452526	15.553113415736636
13	20.246183484862243	25.026302076136865	39.596862700201726	15.130651738799164
14	16.21925173800961	28.12752904578239	39.38131397868989	16.271905237518112
15	15.745913062015656	24.04878270241249	45.48482671225035	14.720477523321504
16	18.77810325020627	22.210104734571093	43.38988855420497	15.621903461017657
17	18.627791573071413	21.565728542835373	39.13704516653705	20.669434717556168
18	19.422282053002878	22.41558662018815	40.72637155704343	17.43575976976554
19	21.35565736438393	22.837061352430055	38.19352603757496	17.613755245611056
20	22.455903311002064	22.993788170086102	37.728329655319435	16.8219788635924
21	21.223110691769275	22.913352177397385	38.808145846739336	17.055391284094004
22	20.606911771091994	21.18412637629438	38.38449983616718	19.824462016446446
23	17.833843912722507	24.786967987461857	38.637404414406234	18.741783685409406
24	17.81548674138496	23.21002775288484	42.075771692057465	16.898713813672735
25	17.695375571934452	24.78440193125338	39.97599750500381	17.54422499180836
26	18.20414556249926	27.001523842609952	37.129254225110245	17.665076369780543
27	17.680176623622717	25.922201123537842	38.86859620934281	17.529026043496625
28	16.559747657980235	24.697698839747815	41.30417832906315	17.438375173208794
29	17.433933922078744	22.511566991832048	41.167486488727114	18.887012597362094
30	18.92762537158468	24.525921115484373	38.89391134078411	17.65254217214684
31	21.67601961256499	24.01192031803306	36.43607361817873	17.87598645122322
32	21.80328613105836	24.684523128061997	36.31393921210231	17.198251528777334
33	20.95051656685366	25.184706699775372	35.57496437129649	18.28981236207448
34	19.258844011416976	25.648077234344097	35.53588136135203	19.55719739288689
35	19.90484866190038	25.69920096957447	34.733445976621255	19.662504391903894
36	22.39111039173809	26.073055620255264	32.844039051427714	18.691794936578933
37	20.340979522871454	28.020100115669916	32.994005297919124	18.6449150635395
38	20.263504364269444	28.67646768545678	31.563280919990365	19.496747030283412
39	20.56215382914803	28.14489927242437	31.985446513519168	19.307500384908433
40	21.524967726908457	27.182085374663945	31.365497202998732	19.927449695428866
41	19.585769441823558	26.546098212840548	31.98386740200626	21.884264943329633
42	20.703336267848897	26.92069307204302	31.03141642354929	21.3445542365588
43	21.247932350862786	26.70524304500073	31.408330602786343	20.638494001350143
44	20.54478360250605	28.4842602059951	30.7216144836108	20.249341707888057
45	19.470296912442215	29.100952599020165	30.44132219006976	20.987428298467865
46	20.582682278815824	28.845037839459632	30.922605770863026	19.649674110861522
47	20.663513049382765	26.827229409372816	32.105755071908796	20.403502469335628
48	21.317462604665486	25.729697560667493	32.118289269542494	20.83455056512453
49	20.41371734693475	25.491202374983718	33.24232058332379	20.852759694757744
50	19.477156178076406	27.62665461278212	32.260656041878036	20.63553316726344
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	571.0
1	1531.5
2	2492.0
3	39993.0
4	77494.0
5	55791.0
6	34088.0
7	33238.0
8	32388.0
9	32699.0
10	33010.0
11	32616.5
12	32223.0
13	31300.5
14	30378.0
15	28659.0
16	26940.0
17	25049.5
18	23159.0
19	21532.5
20	19906.0
21	18715.5
22	17525.0
23	17418.5
24	17312.0
25	18435.0
26	19558.0
27	22132.0
28	24706.0
29	27626.0
30	30546.0
31	36227.5
32	41909.0
33	46758.5
34	51608.0
35	58224.5
36	64841.0
37	71784.5
38	78728.0
39	87721.5
40	96715.0
41	111967.0
42	127219.0
43	136902.0
44	146585.0
45	152957.5
46	159330.0
47	161079.0
48	162828.0
49	156574.5
50	150321.0
51	136360.0
52	122399.0
53	111647.5
54	100896.0
55	91123.0
56	81350.0
57	73288.5
58	65227.0
59	57572.5
60	49918.0
61	43745.5
62	37573.0
63	32095.0
64	26617.0
65	21295.0
66	15973.0
67	12933.5
68	9894.0
69	8098.5
70	6303.0
71	4982.0
72	3661.0
73	2730.5
74	1800.0
75	1373.5
76	947.0
77	852.0
78	757.0
79	591.5
80	426.0
81	300.5
82	175.0
83	140.5
84	106.0
85	68.0
86	30.0
87	17.0
88	4.0
89	7.0
90	10.0
91	6.0
92	2.0
93	2.5
94	3.0
95	4.0
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2026456.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.254427731117694
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.23776339133804	22.59546567245066
2	7.396712416432143	4.3277317766875
3	3.0718304254655684	2.695939235520929
4	1.891101996780954	2.212924267880031
5	1.26571127459073	1.8513829505487682
6	0.9819814269272866	1.7236382812406477
7	0.8094372687662715	1.6575736857397327
8	0.6328541282346009	1.4811028287022896
9	0.5318899950704831	1.400412367952459
>10	5.161472310420924	32.03657814867763
>50	0.7883666718993626	16.029617682650102
>100	0.2274379214561275	8.90861062139357
>500	0.002580579463180178	0.5447601969586394
>1k	6.881545235147142E-4	0.26936036346291925
>5k	0.0	0.0
>10k+	1.7203863087867854E-4	2.26490192013421
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	45002	2.220724259495395	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06469422479441941	0.0
2	0.0	0.0	0.0	0.20750512224297	0.0
3	0.0	0.0	0.0	0.31088757910361736	0.0
4	0.0	0.0	0.0	0.4440264185356109	0.0
5	0.0	0.0	0.0	0.7795876150284042	0.0
6	0.0	0.0	0.0	1.2295850489721958	0.0
7	0.0	0.0	0.0	1.5314420841113747	0.0
8	0.0	0.0	0.0	2.1251880129645055	0.0
9	0.0	0.0	0.0	2.4127343500179625	0.0
10	0.0	0.0	0.0	2.7981856008716695	0.0
11	0.0	0.0	0.0	3.1134650838705604	0.0
12	0.0	0.0	0.0	3.397063642141749	0.0
13	0.0	0.0	0.0	3.5273896891913763	0.0
14	0.0	0.0	0.0	3.5864583292210637	0.0
15	0.0	0.0	0.0	3.6636374044144064	0.0
16	0.0	0.0	0.0	3.80935978871488	0.0
17	0.0	0.0	0.0	4.000777712420107	0.0
18	0.0	0.0	0.0	4.246428247146743	0.0
19	0.0	0.0	0.0	4.373941501814004	0.0
20	0.0	0.0	0.0	4.5256349015226585	0.0
21	0.0	0.0	0.0	4.6843849558046164	0.0
22	4.934723477835196E-5	0.0	0.0	4.881181728100684	0.0
23	4.934723477835196E-5	0.0	0.0	5.089821836743556	0.0
24	4.934723477835196E-5	0.0	0.0	5.250052308068865	0.0
25	4.934723477835196E-5	0.0	0.0	5.390001065900271	0.0
26	4.934723477835196E-5	0.0	0.0	5.532811963348822	0.0
27	4.934723477835196E-5	0.0	0.0	5.6751787356843675	0.0
28	4.934723477835196E-5	0.0	0.0	5.837235054696475	0.0
29	4.934723477835196E-5	0.0	0.0	6.011233404524944	0.0
30	4.934723477835196E-5	0.0	0.0	6.228756015427919	0.0
31	4.934723477835196E-5	0.0	0.0	6.413857493081518	0.0
32	4.934723477835196E-5	0.0	0.0	6.589188218249002	0.0
33	4.934723477835196E-5	0.0	0.0	6.7665915272771775	0.0
34	4.934723477835196E-5	0.0	0.0	6.952285171748116	0.0
35	4.934723477835196E-5	0.0	0.0	7.157076196078276	0.0
36	4.934723477835196E-5	0.0	0.0	7.346865661035818	0.0
37	9.869446955670392E-5	0.0	0.0	7.550817782374747	0.0
38	9.869446955670392E-5	0.0	0.0	7.752993403261655	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTAA	20	7.8579044E-4	44.000004	27
GGCGCGT	20	7.8579044E-4	44.000004	31
CGTTTTT	32900	0.0	43.558662	1
CGACGGT	190	0.0	42.842106	28
CGTTAGG	255	0.0	41.411766	2
CGTTTCG	60	3.6379788E-12	40.333332	42
TCGTTGA	90	0.0	39.11111	24
GCGATAT	225	0.0	39.11111	9
GTTTTTT	37390	0.0	38.845684	2
GACCGAT	820	0.0	38.634148	9
CACGACG	205	0.0	38.634148	26
GCCGATC	40	4.126614E-7	38.500004	9
TAACGGG	515	0.0	38.4466	3
TGGGCGA	865	0.0	38.150288	6
AGGGCGA	1485	0.0	37.925926	6
CGGTCTA	210	0.0	37.714287	31
TCGCAAG	70	0.0	37.714283	1
GGACCGA	905	0.0	37.679558	8
ACGGGAC	775	0.0	37.470966	5
CCGCAAT	65	1.0913936E-11	37.23077	33
>>END_MODULE