FastQCFastQC Report
Thu 26 May 2016
SRR1545878_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545878_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences351651
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21710.6173734754060133No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA4760.13536148055884953No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTGA408.283678E-944.024
ATTAGCG408.283678E-944.01
ATAATCG207.849022E-444.025
CGTTGAT408.283678E-944.025
ATTAACG207.849022E-444.01
GCGGGTC351.4422585E-744.05
CGTAAGG351.4422585E-744.02
CATCGCA207.849022E-444.043
CGTCGGG207.849022E-444.03
ATACGGA207.849022E-444.03
TCGACGG254.4365202E-544.02
TATCGAG207.849022E-444.01
ATCGATA207.849022E-444.028
AGTAACG207.849022E-444.01
AATCGAT207.849022E-444.027
GTACGAG254.4365202E-544.01
TAATCGA207.849022E-444.026
ACGCGGA207.849022E-444.011
CGTTTTT18850.041.7824941
CGGGATG1700.041.4117626
Produced by FastQC (version 0.11.5)