FastQCFastQC Report
Thu 26 May 2016
SRR1545876_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545876_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1111565
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142721.2839555041765438No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA47860.4305641145592026No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGACG207.856369E-444.0000048
CTTCGAT207.856369E-444.00000430
TCGTTTA207.856369E-444.00000438
CGAACGT408.312782E-944.00000438
ACACGAA207.856369E-444.00000410
CTCCGAT207.856369E-444.00000436
GCGAATC207.856369E-444.00000424
CGACGAA207.856369E-444.00000427
CTATCCG207.856369E-444.0000041
GCGCGAT207.856369E-444.00000419
ATTACGC207.856369E-444.00000425
TTGTCGT207.856369E-444.00000440
CACGCGA207.856369E-444.00000420
CGCGCCA207.856369E-444.00000438
TAACGTG207.856369E-444.0000042
TCCTACG408.312782E-944.0000041
AGAACGT207.856369E-444.00000429
AATATCG207.856369E-444.00000425
ATCGTAC207.856369E-444.00000410
CGTTACC207.856369E-444.00000418
Produced by FastQC (version 0.11.5)