##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545876_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1111565
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.678642274630814	31.0	31.0	33.0	30.0	34.0
2	32.09108869027003	33.0	31.0	34.0	30.0	34.0
3	32.06884257780697	33.0	31.0	34.0	30.0	34.0
4	35.83785923450271	37.0	35.0	37.0	35.0	37.0
5	35.80740307584352	37.0	35.0	37.0	35.0	37.0
6	35.852323525839694	37.0	35.0	37.0	35.0	37.0
7	36.103669151151756	37.0	35.0	37.0	35.0	37.0
8	36.11241447868546	37.0	35.0	37.0	35.0	37.0
9	37.92374535002452	39.0	38.0	39.0	35.0	39.0
10	37.50596411365957	39.0	37.0	39.0	35.0	39.0
11	37.22932981876903	39.0	37.0	39.0	34.0	39.0
12	36.308763769999956	38.0	35.0	39.0	33.0	39.0
13	35.92443626778461	38.0	35.0	39.0	32.0	39.0
14	36.920451795441565	39.0	35.0	41.0	32.0	41.0
15	37.12328383855195	39.0	35.0	41.0	33.0	41.0
16	37.335678975138656	39.0	35.0	41.0	33.0	41.0
17	37.28769977464206	38.0	35.0	41.0	33.0	41.0
18	37.11302083099054	38.0	35.0	40.0	33.0	41.0
19	36.93862887010656	37.0	35.0	40.0	33.0	41.0
20	36.64452101316612	36.0	35.0	40.0	33.0	41.0
21	36.48543719890425	36.0	35.0	40.0	32.0	41.0
22	36.36786062893308	36.0	35.0	40.0	32.0	41.0
23	36.34800214112535	36.0	35.0	40.0	32.0	41.0
24	36.323675178689506	36.0	35.0	40.0	32.0	41.0
25	36.30248343551659	36.0	35.0	40.0	32.0	41.0
26	36.09811392046349	35.0	35.0	40.0	32.0	41.0
27	36.081271900428675	36.0	35.0	40.0	31.0	41.0
28	36.099311331321154	36.0	35.0	40.0	31.0	41.0
29	36.08881621857471	36.0	35.0	40.0	31.0	41.0
30	35.974314592488966	36.0	35.0	40.0	31.0	41.0
31	35.67532083144036	36.0	35.0	40.0	31.0	41.0
32	35.3192642805414	35.0	35.0	40.0	30.0	41.0
33	34.904844970829416	36.0	34.0	40.0	27.0	41.0
34	34.516099373405964	36.0	34.0	40.0	23.0	41.0
35	34.169302739830776	35.0	34.0	40.0	23.0	41.0
36	33.97273033965625	35.0	34.0	40.0	21.0	41.0
37	33.83150243125683	35.0	33.0	40.0	20.0	41.0
38	33.801440311632696	35.0	33.0	40.0	21.0	41.0
39	33.71366586749313	35.0	33.0	40.0	20.0	41.0
40	33.5534566129736	35.0	33.0	40.0	18.0	41.0
41	33.55900284733686	35.0	33.0	40.0	18.0	41.0
42	33.515176350460834	35.0	33.0	40.0	18.0	41.0
43	33.41843257029503	35.0	33.0	40.0	18.0	41.0
44	33.286561739529404	35.0	33.0	40.0	18.0	41.0
45	33.207182665880985	35.0	33.0	40.0	18.0	41.0
46	33.15692559589408	35.0	33.0	40.0	18.0	41.0
47	33.109647209115074	35.0	33.0	40.0	18.0	41.0
48	33.055601786670145	35.0	33.0	40.0	18.0	41.0
49	33.022965818463156	35.0	33.0	40.0	18.0	41.0
50	32.82870727307895	35.0	33.0	39.0	17.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	1.0
13	3.0
14	9.0
15	22.0
16	54.0
17	138.0
18	386.0
19	758.0
20	1521.0
21	2570.0
22	4020.0
23	6243.0
24	9797.0
25	16240.0
26	24966.0
27	31373.0
28	31117.0
29	27854.0
30	27961.0
31	31664.0
32	38876.0
33	52000.0
34	101584.0
35	193628.0
36	62995.0
37	78219.0
38	127770.0
39	239795.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.754715198841275	21.28152649642621	28.80686239671094	20.156895908021575
2	36.14480484721991	23.57046146649094	28.432165460409426	11.852568225879729
3	26.619046119660116	22.657514405365408	37.728967716687734	12.99447175828674
4	23.991219586798792	25.233612069469626	36.37358139200137	14.40158695173022
5	21.660901521728373	29.264055633273806	36.073913806210165	13.001129038787656
6	19.66857538695443	36.908502876574914	32.94598156653007	10.47694016994058
7	80.5804428890798	4.409728625856338	13.403084839842924	1.6067436452209272
8	82.59507990985682	2.8209776306378846	12.98115719728491	1.6027852622203829
9	78.3586205035243	4.833635459914625	14.41535132898211	2.3923927075789537
10	47.64246805180084	25.141309774956934	18.834795985839783	8.381426187402447
11	47.25625581949773	16.953664428081126	25.68261865028136	10.107461102139775
12	41.66791865522934	19.365579160912766	27.58075326229235	11.38574892156554
13	20.404564735305627	38.72351144557448	28.484164218916575	12.387759600203317
14	13.676573119880528	40.946143500380096	32.60430114298309	12.772982236756286
15	12.866274127019112	21.39991813344249	54.57692532600432	11.15688241353407
16	14.307485392217279	16.693310782545332	52.746533041252654	16.252670783984744
17	14.913207954550566	15.981521548447459	34.515750315995916	34.58952018100606
18	19.298556539653553	20.56496921007768	40.901791618123994	19.23468263214477
19	27.639589227800442	20.484811954316662	32.54870385447545	19.326894963407447
20	29.744909204589927	19.969862311245855	31.01941856751517	19.265809916649047
21	20.74732471785276	25.289569210977312	34.4770661184906	19.48603995267933
22	20.96188706913226	22.619819803610223	31.66157624610347	24.75671688115405
23	18.3301921165204	27.848753784079207	30.52156194194671	23.29949215745368
24	18.620413561060307	20.4232770913082	44.72846842065017	16.227840926981326
25	14.461682402738482	22.923175882651936	42.45743613733789	20.157705577271685
26	15.116165046578473	32.139101177169124	34.1702014726984	18.574532303553998
27	16.021645157952978	31.819011933625113	34.71465906177327	17.444683846648644
28	13.368808841588212	26.242459955108338	44.77084111140599	15.61789009189746
29	14.075470170435377	21.509493371957554	44.6945522753955	19.720484182211568
30	16.92100776832664	27.105477412477004	37.843490933953476	18.13002388524288
31	26.401065164880144	24.45992811936324	30.323732755169512	18.8152739605871
32	28.41480255315704	24.903177052174186	31.743892619864784	14.938127774803991
33	26.06829110308439	26.234363262607225	29.99986505512498	17.697480579183402
34	19.805409490223244	26.44451741463612	31.800389540872555	21.94968355426808
35	18.417456469032402	25.327443739232525	34.42497739673344	21.83012239500164
36	29.265045229023944	22.280388461313553	30.351981215673398	18.102585093989106
37	20.605722562333288	30.225582849406017	31.138799800281586	18.02989478797911
38	20.625334550835984	31.038580739767806	26.49606635689321	21.840018352503
39	20.661319850840933	30.368894306675724	30.314826393418286	18.654959449065057
40	23.20296159019041	25.937124684566353	28.507554663919787	22.352359061323448
41	17.07178617534737	24.84335149091596	30.14524566714497	27.9396166665917
42	22.878284220895765	25.19222897446393	26.448835650636717	25.480651154003592
43	22.436114847084966	27.296109539253216	27.04925038121927	23.21852523244255
44	19.693585170457865	31.24936463454679	29.338365277784025	19.71868491721132
45	17.397992919892225	36.076072924210465	25.10901296820249	21.416921187694825
46	21.40594567119332	32.242289024933314	28.363073684399925	17.988691619473446
47	22.015716579777163	27.13687458673132	28.708082748197363	22.139326085294158
48	23.432997620472037	23.4159945662197	31.722841219361893	21.428166593946372
49	20.374517009801497	22.946656290905164	34.61354036875936	22.065286330533976
50	18.43293014803453	32.17517644042409	29.314435053280736	20.07745835826065
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	377.0
1	904.5
2	1432.0
3	13725.5
4	26019.0
5	19127.0
6	12235.0
7	12656.0
8	13077.0
9	13768.5
10	14460.0
11	14114.5
12	13769.0
13	12765.5
14	11762.0
15	10716.5
16	9671.0
17	8608.0
18	7545.0
19	7060.0
20	6575.0
21	5973.5
22	5372.0
23	6078.0
24	6784.0
25	6619.0
26	6454.0
27	7775.5
28	9097.0
29	9928.5
30	10760.0
31	14707.5
32	18655.0
33	18956.5
34	19258.0
35	23938.5
36	28619.0
37	31998.0
38	35377.0
39	44339.0
40	53301.0
41	69223.5
42	85146.0
43	99544.5
44	113943.0
45	112348.0
46	110753.0
47	102724.0
48	94695.0
49	89804.0
50	84913.0
51	81467.5
52	78022.0
53	69543.5
54	61065.0
55	52414.5
56	43764.0
57	39778.0
58	35792.0
59	32973.5
60	30155.0
61	26965.0
62	23775.0
63	19668.0
64	15561.0
65	12590.0
66	9619.0
67	7795.0
68	5971.0
69	4585.0
70	3199.0
71	2592.5
72	1986.0
73	1617.0
74	1248.0
75	970.0
76	692.0
77	558.5
78	425.0
79	266.0
80	107.0
81	88.0
82	69.0
83	51.0
84	33.0
85	24.0
86	15.0
87	14.0
88	13.0
89	7.0
90	1.0
91	0.5
92	0.0
93	1.5
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1111565.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.126091381799437
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.2754403635355	14.913321505072341
2	6.586787145200343	2.3878541141272644
3	2.112196533171516	1.1485760212976057
4	1.0134524855364688	0.734797294557832
5	0.6451891000118045	0.5847378292677451
6	0.46478129162959003	0.5054800898777234
7	0.3428776373656791	0.43505219713660515
8	0.2951976131256579	0.42806231289638
9	0.25279124635486927	0.41239055087526005
>10	3.180446102261026	14.289606956132214
>50	1.3139600249128425	17.317824725432914
>100	1.5143690654098267	44.94458729217865
>500	0.0015068348909550516	0.162603553660976
>1k	5.022782969850171E-4	0.4357338229683265
>5k	0.0	0.0
>10k+	5.022782969850171E-4	1.2993717345181688
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14272	1.2839555041765438	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	4786	0.4305641145592026	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0383243445052696	0.0
2	0.0	0.0	0.0	0.13728391951887653	0.0
3	0.0	0.0	0.0	0.19324106102657065	0.0
4	0.0	0.0	0.0	0.28032548703854476	0.0
5	0.0	0.0	0.0	0.44072996181060037	0.0
6	0.0	0.0	0.0	0.5886295448309365	0.0
7	0.0	0.0	0.0	0.680661949593591	0.0
8	0.0	0.0	0.0	0.947672875630305	0.0
9	0.0	0.0	0.0	1.079469036898427	0.0
10	0.0	0.0	0.0	1.3409922046843865	0.0
11	0.0	0.0	0.0	1.544669002712392	0.0
12	0.0	0.0	0.0	1.751134661490781	0.0
13	0.0	0.0	0.0	1.8237350042507636	0.0
14	0.0	0.0	0.0	1.8482949715041406	0.0
15	0.0	0.0	0.0	1.8911174785100286	0.0
16	0.0	0.0	0.0	1.9905268697736975	0.0
17	0.0	0.0	0.0	2.1012716305389247	0.0
18	0.0	0.0	0.0	2.2872256683144934	0.0
19	0.0	0.0	0.0	2.351819281823375	0.0
20	0.0	0.0	0.0	2.431526721334335	0.0
21	0.0	0.0	0.0	2.5240089423470513	0.0026988975003710983
22	0.0	0.0	0.0	2.6065952058584068	0.0026988975003710983
23	0.0	0.0	0.0	2.696108639620715	0.0026988975003710983
24	0.0	0.0	0.0	2.7626814446298686	0.0026988975003710983
25	0.0	0.0	0.0	2.821067593887897	0.0026988975003710983
26	0.0	0.0	0.0	2.882422530396333	0.0026988975003710983
27	0.0	0.0	0.0	2.9430577609046704	0.0026988975003710983
28	0.0	0.0	0.0	3.0053123299132305	0.0026988975003710983
29	0.0	0.0	0.0	3.0685564946719266	0.0026988975003710983
30	0.0	0.0	0.0	3.163017907184915	0.0026988975003710983
31	0.0	0.0	0.0	3.2530711204472973	0.0026988975003710983
32	0.0	0.0	0.0	3.319643925456451	0.0026988975003710983
33	0.0	0.0	0.0	3.390175113466149	0.0026988975003710983
34	0.0	0.0	0.0	3.4646646844763915	0.0026988975003710983
35	0.0	0.0	0.0	3.5633543697399612	0.0026988975003710983
36	0.0	0.0	0.0	3.6484596042516633	0.0026988975003710983
37	0.0	0.0	0.0	3.7306860147629695	0.0026988975003710983
38	0.0	0.0	0.0	3.8189399630251044	0.0026988975003710983
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTGACG	20	7.856369E-4	44.000004	8
CTTCGAT	20	7.856369E-4	44.000004	30
TCGTTTA	20	7.856369E-4	44.000004	38
CGAACGT	40	8.312782E-9	44.000004	38
ACACGAA	20	7.856369E-4	44.000004	10
CTCCGAT	20	7.856369E-4	44.000004	36
GCGAATC	20	7.856369E-4	44.000004	24
CGACGAA	20	7.856369E-4	44.000004	27
CTATCCG	20	7.856369E-4	44.000004	1
GCGCGAT	20	7.856369E-4	44.000004	19
ATTACGC	20	7.856369E-4	44.000004	25
TTGTCGT	20	7.856369E-4	44.000004	40
CACGCGA	20	7.856369E-4	44.000004	20
CGCGCCA	20	7.856369E-4	44.000004	38
TAACGTG	20	7.856369E-4	44.000004	2
TCCTACG	40	8.312782E-9	44.000004	1
AGAACGT	20	7.856369E-4	44.000004	29
AATATCG	20	7.856369E-4	44.000004	25
ATCGTAC	20	7.856369E-4	44.000004	10
CGTTACC	20	7.856369E-4	44.000004	18
>>END_MODULE